LOCUS       sequence-38110-e        6591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pRS2 (CK2alpha', CK2beta)
FEATURES             Location/Qualifiers
     source          1..6591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(136..154,155..177,178..196,197..219,220..238,239..261,
                     262..280,281..303,304..322,323..345,346..364,365..387,
                     388..406)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        438..475
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     640..656
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     640..656
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             673..702
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          1428..2000
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2132..2151)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2197..2252
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2251..2270)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2643..2660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2814..3402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2894..2913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3573..4364,4365..4433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4196..4215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4434..4538)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4606..4624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    4969..5103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5006..5025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5060..5079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6465..6554)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa
       61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata
      121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag gaaagtcgag
      421 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      481 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      541 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
      601 tcagctgacg cgtgctagcg cggcctcgac gatggccgct ctagaactag tggatccacc
      661 atggcgtcta tggaacaaaa gctgattagc gaagaggacc tgaacaacgg gaattctcga
      721 gcagccgctg acgtgaagat gagcagctca gaggaggtgt cctggatttc ctggttctgt
      781 gggctccgtg gcaatgaatt cttctgtgaa gtggatgaag actacatcca ggacaaattt
      841 aatcttactg gactcaatga gcaggtccct cactatcgac aagctctaga catgatcttg
      901 gacctggagc ctgatgaaga actggaagac aaccccaacc agagtgacct gattgagcag
      961 gcagccgaga tgctttatgg attgatccac gcccgctaca tccttaccaa ccgtggcatc
     1021 gcccagatgt tggaaaagta ccagcaagga gactttggtt actgtcctcg tgtgtactgt
     1081 gagaaccagc caatgcttcc cattggcctt tcagacatcc caggtgaagc catggtgaag
     1141 ctctactgcc ccaagtgcat ggatgtgtac acacccaagt catcaagaca ccatcacacg
     1201 gatggcgcct acttcggcac tggtttccct cacatgctct tcatggtgca tcccgagtac
     1261 cggcccaaga gacctgccaa ccagtttgtg cccaggctct acggtttcaa gatccatccg
     1321 atggcctacc agctgcagct ccaagccgcc agcaacttca agagcccagt caagacgatt
     1381 cgctgattcc ctcgaggggg ggccatctct agactgagaa cttcagggtg agtttgggga
     1441 cccttgattg ttctttcttt ttcgctattg aaaaattcat gttatatgga gggggcaaag
     1501 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     1561 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     1621 cattttctgt aacttttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     1681 actttcgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     1741 cagggtaatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     1801 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     1861 acaactacat cctggtaatc atcctgcctt tctctttatg gttacaatga tatacactgt
     1921 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     1981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt
     2041 ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2101 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2161 acatcatgaa gccccttgag catctgactt ctgggtaata aaggaaattt attttcattg
     2221 caatagtgtg tgggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     2281 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc
     2341 tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2401 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2461 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2521 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg aactcgactg
     2581 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2761 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3001 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3241 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3541 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3601 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3661 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3721 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3781 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3841 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3901 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3961 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4021 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4081 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4141 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4201 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4261 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4321 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4381 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4441 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4501 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4561 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4621 aggccctttc gtcttcactc gacgctggtc gagctgatac ttcccgtccg ccaggggaca
     4681 tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga ggccggttac cgcctgttga
     4741 cctggtggga cgggcagggc gccgcccgag tcttgcctcg gcggcgggcg ctctgctcat
     4801 ggagcgcgcg tccggggacc ttgcacagat agcgtggtcc ggccagacga cgaggcttgc
     4861 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4921 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5101 atcctctaga actaggtcga agcttgatat cgaattcctg cagcccgggg gatccacctg
     5161 caggtaattt ttcagggaat cccctgagtt atgaaaagtt cgagttaaaa aaaaaaagaa
     5221 aaaagaaaat cagcctatta taattttttt tttatgactg aactactata aatccacaag
     5281 caacggttca gacacggtgc ttctgaagtg tttcacccct ccccgccagg cgcaagctgc
     5341 atcaaggaga gggtggactc ccccacctct gctcaggcat caggagacag atagggccgt
     5401 catcgtgctg ccgtgagacc actggaaagc acagcattgt ctgcacaagg ctgggactgc
     5461 tccttcacca cagggtagaa gtatgggtgc tccatggcct ctttggcagt cagtctctgt
     5521 tgatggtcgt atcgcagaag tttgtccaga agatctaggg cctcagggct gacaaggtgt
     5581 ctgttctcac tatggataaa gttttcccag cgtttccgtg aatgttgtcc caggatatcg
     5641 ttgaagtgtg gatctaggtc tatgtgatac ttcttcagat acccatacag ttcttctgta
     5701 cccagaacct tggcaatgcg aacaagctgg tcatagttgt cctgtccatg gaagaatggt
     5761 tcccttcgaa agatcatgct tgctaacata cagcccaaac tccacatgtc caagctataa
     5821 tcatacatct gatagtccac gaggagctct ggtcccttga agtaccttga ggctacacga
     5881 acattgtact cctgagcagg atgatagaat tctgccagac cccaatctat cagtcgcagc
     5941 tttttctgtt ggtgatctat catgacattg tgaggtttca catccctgtg catgattccc
     6001 ttgctgtggc agtaatccag agctttaagt agttcataca tataaaaccg gatatcaaag
     6061 tctgtcagga tctggtagag ttgcttaaaa tctgtattat tgatatattc aaataccaaa
     6121 gctggtgtct ttgacacggg gtcctttaca gtgtcaatca gcttaatgat atttgttcca
     6181 ccacgaaggt tctccagaat cttaacctct cgttttatct tctttttctt cactggcttc
     6241 aggattttta caaccactct ctcattgttg gtgatattaa tggcctcaaa tacttcacta
     6301 tattttcccc gaccaagttt tcgaaccagt tggtaatcat cttgattacc ccagctcggg
     6361 acgtgagcct cgtagtccca gtactcgcgg ctcctcagac tgttcacctc ggcgtagacc
     6421 cgggccctgc tgcccgcggc cgggccgggg cggccgcact gagcagcgta atctggaacg
     6481 tcatatggat aggatcctgc atagtccggg acgtcatagg gatagcccgc atagtcagga
     6541 acatcgtatg ggtaaaagat gcggcccatg gtggaagctt cgacgcggcc g
//
