LOCUS       sequence-381187-        6303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TIR1 expression vector under the unc-4c promoter expressed in the DA
            motor neurons in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Kurashina M, Mizumoto K
  TITLE     Targeting endogenous proteins for spatial and temporal knockdown 
            using auxin-inducible degron in Caenorhabditis elegans.
  JOURNAL   STAR Protoc. 2023 Jan 13;4(1):102028. doi: 
            10.1016/j.xpro.2022.102028.
  PUBMED    36640369
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2023-01-13"; volume: "4(1):102028. doi"; pages: " 
            10.1016/j.xpro.2022.102028"
COMMENT     Sequence Label: Punc-4c.TIR1
FEATURES             Location/Qualifiers
     source          1..6303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3822..3844)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3944..3963
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4045..4063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4131..4235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4236..4304,4305..5096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4454..4473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5267..5855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5756..5775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6009..6026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6143..6164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6179..6184,6185..6202,6203..6209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6217..6233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6222..6244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6241..6257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6241..6257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgcgcggcc gcactgactg ggccggccac tgactggtga tccatctcga atttctcgtt
       61 tcaaattagt cgttgctata tatattgtct tatgtgtgga aaacgaataa gaaaaattag
      121 agaaagatga gagagcaggg gcagaatgaa aatttgcatg gaaattgaag aagatcactg
      181 ctttttccga tgaatgatga ctaaatagaa tgagttgaga ggaaaataat cataattcta
      241 attttaatta gaaatatgat atggaaagta agcaaaacca caaggctttt gttctgaact
      301 tatttccgaa aaaaaaagtg aaactgaaag ttagaatttt caaaataaat attgcgagag
      361 tcaaccttca gaaaaattgg cgagaaatca aatttgaatt ctctgaaaat ttagttaaaa
      421 tacatatgtt atccttcttt gttttttggc cttagcctgc cttcaaggcg acctgagcct
      481 atgcgggtgc ctaataccga aacacacata attttttttt tttaatcttt aaatactgta
      541 acgagtcgcg agatccagcg agacccatag aatatgttga gcgctgcaaa caatcgtata
      601 actcttgtcc aaatttttcc actctgtatt tttgcaagca gttcaacaaa tggtcatcat
      661 ttttataacc catatctata ttcgaatctg acataacaaa taatactaag cacatcctcc
      721 cacatctctt ttcattcaag ttgcgcaatt cccctaataa ctatggatgg aggggggacc
      781 gagcccaaag attattcaaa acgataacga ctttttcatg cggccaacga aaaataaaac
      841 ttttctcata ttctcctatt tcccatttca agaattacat ccccatggga cgttgaaatc
      901 acatcatatt agtatatgtt aattgttctt ttcactggaa tatcgccacg ccccccttcc
      961 ggtggctcat tggtttcgag aagaaggcgg agccgaagtg ggttcttctt tctttttttt
     1021 gtggtagaag tctagcagga ttaagctact attccattag aggatcttct tcttccaaaa
     1081 agtgaaaggc gcgccatgca aaagagaatc gccttgtcgt ttccagaaga agtactcgag
     1141 cacgtcttct ccttcatcca actcgacaag gaccgtaact ccgtctccct cgtctgcaag
     1201 tcctggtacg agatcgagcg ttggtgccgt cgtaaggtct tcatcggaaa ctgctacgcc
     1261 gtctccccag ccaccgtcat ccgtcgtttc ccaaaggtcc gttccgtcga gctcaaggga
     1321 aagccacact tcgccgactt caacctcgtc ccagacggat ggggaggata cgtctaccca
     1381 tggatcgagg ccatgtcctc ctcctacacc tggctcgagg agatccgtct caagcgtatg
     1441 gtcgtcaccg acgactgcct cgagctcatc gccaagtcct tcaagaactt caaggtcctc
     1501 gtcctctcct cctgcgaggg attctccacc gacggactcg ccgccatcgc cgccacctgc
     1561 cgtaacctca aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     1621 caggagctcg acctccgtga gtccgacgtc gacgaggtct ccggacactg gctctcccac
     1681 ttcccagaca cctacacctc cctcgtctcc ctcaacatct cctgcctcgc ctccgaggtc
     1741 tccttctccg ccctcgagcg tctcgtcacc cgttgcccaa acctcaagtc cctcaagctc
     1801 aaccgtgccg tcccactcga gaagctcgcc accctcctcc aacgtgcccc acaactcgag
     1861 gagctcggaa ccggaggata caccgccgag gtccgtccag acgtctactc cggactctcc
     1921 gtcgccctct ccggatgcaa ggtaagttta aacagttcgg tactaactaa ccatacatat
     1981 ttaaattttc aggagctccg ttgcctctcc ggattctggg acgccgtccc agcctacctc
     2041 ccagccgtct actccgtctg ctcccgtctc accaccctca acctctccta cgccaccgtc
     2101 caatcctacg acctcgtcaa gctcctctgc caatgcccaa agctccaacg tctctgggtc
     2161 ctcgactaca tcgaggacgc cggactcgag gtcctcgcct ccacctgcaa ggacctccgt
     2221 gagctccgtg tcttcccatc cgagccattc gtcatggagc caaacgtcgc cctcaccgag
     2281 caaggactcg tctccgtctc catgggatgc ccaaagctcg agtccgtcct ctacttctgc
     2341 cgtcaaatga ccaacgccgc cctcatcacc atcgcccgta accgtccaaa catgacccgt
     2401 ttccgtctct gcatcatcga gccaaaggcc ccagactacc tcaccctcga gccactcgac
     2461 atcggattcg gagccatcgt cgagcactgc aaggacctcc gtcgtctctc cctctccgga
     2521 ctcctcaccg acaaggtctt cgagtacatc ggaacctacg ccaagaagat ggagatgctc
     2581 tccgtcgcct tcgccggaga ctccgacctc ggactccacc acgtcctctc cggatgcgac
     2641 tccctccgta agctcgagat ccgtgactgc ccattcggag acaaggccct cctcgccaac
     2701 gcctccaagc tcgagaccat gcgttccctc tggatgtcct cctgctccgt ctccttcgga
     2761 gcctgcaagc tcctcggaca aaagatgcca aagctcaacg tcgaggtcat cgacgagcgt
     2821 ggagccccag actcccgtcc agagtcctgc ccagtcgagc gtgtcttcat ctaccgtacc
     2881 gtcgccggac cacgtttcga catgccagga ttcgtctgga acatggacca agactccacc
     2941 atgcgtttct cccgtcaaat catcaccacc aacggactct aaggtaccat ggtattgata
     3001 tctgagctcc gcatcggccg ctgtcatcag atcgccatct cgcgcccgtg cctctgactt
     3061 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     3121 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     3181 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     3241 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     3301 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     3361 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
     3421 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
     3481 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
     3541 tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc
     3601 acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag
     3661 tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct
     3721 taaatgacag aatacattcc caatatacca aacataactg tttctactag tcggccgtac
     3781 gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3841 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3901 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     3961 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4021 cgcatcaggc ggccttaatt aagggcctcg tgatacgcct atttttatag gttaatgtca
     4081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6061 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6241 caggaaacag ctatgaccat gattacgcca agcttgcawk srcsrysryw mygmcwrgsy
     6301 ygc
//
