LOCUS       sequence-381189-        8564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mCherry2-Hygro to C-terminus of 
            endogenous human eIF3k.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
FEATURES             Location/Qualifiers
     source          1..8564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    98..119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(134..139,140..157,158..164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     177..199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     196..212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     196..212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        230..248
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     230..247
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1128..1331
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             1338..2048
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1483..1501)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    2166..2287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2203..2222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2257..2276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2318..2351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2412..2911
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2447..2470)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2844..2863
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2972..3991
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             2972..3991
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    4554..4587
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5443..5462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5444..5462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5469..5486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5469..5485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5478..5500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5623..5925
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5804..5823
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6274..7068
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6274..7068
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6328..6347)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6938..6957
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7268..7277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7274..7648
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7786..8374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8275..8294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8528..8545
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
       61 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      181 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta tttaggtgac
      241 actatagaat actcaagcta tgcatcaagc ttggtaccac tagtaacggc cgccagtgtg
      301 ctggaattcg cccttagggc ctcctttctt ctcccctttg gcttctgttg ccccacccca
      361 ccttcctgac cttttaggtc acaggtggag agggcagtgc tgagtcaaca aggaaaagga
      421 ggaggaaggt gtgaatctca gggaagtagg agcccagccc tgccggggga gtagacggga
      481 gggttccctc tgtctcagtt ctggctccat tgctctgtgt cctgggtcaa acaggatagg
      541 ttcaaaatac tcaggaggct gaggcaggag aatcacttga acccggaagg tggaggttgc
      601 agtgagccaa gatcgctcca ctgcactaca gcctgggcaa cagaatgaga ctgtgtctca
      661 aaaaaaaact taagagtatc ctgcaaatag tacctagtaa atattatcat gtaggtaatt
      721 acaaacaaca cttccttggc actttgttct aagcacctcg tttggtccta caatcctata
      781 aggtaggtgc ttcttaaaga taggaaggta ggtgatgctc agagatgttc agtaacttgt
      841 ccaagtgacc cagctgttaa ttggcagagc tgggatttga acctagccct ccttgctcca
      901 gagtccatct ttttaaaata ctgcaccaaa ctgcctttgt gatggtaact ggggcctgga
      961 aggagtttat gatctccccc taaagaattg tgggtgctgt agcaggtggc tggaggtttg
     1021 tctcccgccc ttctcacctt cagtctggtc tctccttccc cacaggtgtg agcagtatca
     1081 tggcatcctc ccagggagga ggaggaggag gatccggtgc aggcgccaag gagaagagtg
     1141 cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga tggccaccgg
     1201 tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt ggcccggagg
     1261 cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa atcgatttga
     1321 ggatgtataa agctagcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1381 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1441 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1501 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1561 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     1621 gcttcaattg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1681 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1741 cctccgacgg ccccgtaatg cagtgtcgta ccatgggctg ggaggcctcc actgagcgga
     1801 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1861 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1921 ccggcgccta caacgtcgac atcaagttgg acatcctttc ccacaacgag gactacacca
     1981 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2041 acaagtaatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     2101 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2161 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2281 tatcttaacg cgtcgatcat attcaataac ccttaatata acttcgtata atgtatgcta
     2341 tacgaagtta ttaggtctga agaggagttt acgtccagcc aagcttagga tctcgacctc
     2401 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2461 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2521 caccggtagg cgccaaccga ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2581 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2641 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2701 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     2761 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     2821 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     2881 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
     2941 gaagcttacc gctagcatgg atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga
     3001 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     3061 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     3121 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     3181 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     3241 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     3301 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     3361 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     3421 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     3481 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     3541 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     3601 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     3661 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     3721 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     3781 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     3841 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     3901 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     3961 ccccagcact cgtccgaggg caaaggaata ggctagccgc ccgccccacg acccgcagcg
     4021 cccgaccgaa aggagcgcac gaccccatgc atcgatgata tcagatcccc gggatgcaga
     4081 aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac
     4141 tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg
     4201 gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt
     4261 gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag
     4321 ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg
     4381 tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt
     4441 catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg
     4501 ttttgccaag ttctaattcc atcagaagct ggtcgagatc cggaaccctt aatataactt
     4561 cgtataatgt atgctatacg aagttattag gtccctcgaa gaggttcact aggatcctaa
     4621 cttcaggtgt ttaataaaga tgtgttgact cagccctact gtctcctccc tggcttcctg
     4681 cctctgcccc ttgcccacca gagggagctc aggagcaggc ctctgctctg ggttggcccc
     4741 cgcaccgtac ccccagcccc caccacacca caccccatgg cggggtttcc gcctcctttt
     4801 tcccctgctt cgacttcctc ccttgcccca ccctaccttc ctgacctttt aggtcttggg
     4861 tggggagggc agcattgagt cagcaaggag gagggagtat gaatgggatg tggcagccca
     4921 gccctgtggt ggaggctgag cacctctggg aggagtgaag gtgaagtttc cctctgtctt
     4981 gatggtggct cttgctttgt gaccttggtc aagggactgt gcctcttttt tttttagacg
     5041 aagtctcgct ctgtcgccca ggctggagtg cagtcgcgcg atctcggctc actgcacgct
     5101 ccacctcccg ggtttatgcc attctcctgc ctcagcctcc agagtagctg ggactacagg
     5161 cgcccgccac catgctcggc caatttcttg tatttttagt agacagggtt tcaccgtgtt
     5221 agccaggatg gtcttgatct cctgaccttg taatctgcct gcctcggcct cccaaagtac
     5281 tgggattaca ggcgtgagcc accgcgccca gccgggattg tgcctctcta attctcagtt
     5341 tcttcatctg cagaagggga agaataatgc accaagggcg aattctgcag atatccatca
     5401 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat
     5461 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5521 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5581 accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa ggtttacacc
     5641 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     5701 ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     5761 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     5821 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     5881 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggcatgaga
     5941 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg
     6001 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     6061 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     6121 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     6181 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc
     6241 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6301 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6361 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6421 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6481 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6541 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6601 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6661 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6721 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6781 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     6841 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6901 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6961 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7021 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tattaacgct
     7081 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
     7141 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatagcacgt
     7261 gaggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     7321 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     7381 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     7441 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     7501 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     7561 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     7621 gcacttcgtg gccgaggagc aggactgaca cgtgctaaaa cttcattttt aatttaaaag
     7681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaa
//
