LOCUS       sequence-381191-        8569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mClover-NeoR to C-terminus of 
            endogenous human eIF3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3a- pMK289 (mAID-mClover-NeoR) plasmid
FEATURES             Location/Qualifiers
     source          1..8569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    75..96
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(111..116,117..134,135..141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     154..176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        207..225
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     207..224
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1237..1256
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1444..2238)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1444..2238)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2587..2889)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2689..2708)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3012..3034
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3026..3043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3027..3043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3050..3069
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3050..3068
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4101..4304
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(4311..4313,4314..4316,4317..5030)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4356..4377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4964..4985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5148..5269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5185..5204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5239..5258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5276..5731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5413..5434)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5625..5644
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5758..5862
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5864..6221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5864..6055
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5887..5907)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6072..6207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6256..7050
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6256..7050
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      7250..7259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7256..7630
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7768..8356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8257..8276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8510..8527
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
       61 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      121 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      181 agctatgacc atgattacgc caagctattt aggtgacact atagaatact caagctatgc
      241 atcaagcttg gtaccactag taacggccgc cagtgtgctg gaattcgccc ttattactac
      301 aaattacttg ttgaaagaat ctatcttcaa aagaaagaaa attaggaaag agaatttaaa
      361 cgattcctcc aaaccataca tttataaata ttgtcatatt taaaatgctt gaaaggcatg
      421 aattctccat taatggaaaa gcagtttgct attggcaaca gaaaaaaact cagcgataat
      481 ttaccaaatg ctttaaaatt taaaagctat actgtttgga ttcacacttg cgcagtaatc
      541 ttgtcattac aacaactgca aattgctttg tactcacctt ctctcaagta aaatctaaag
      601 aaaaccccac cgtatccaat gtttgtaatg tttacttcaa gtgaatctaa aaaatttcta
      661 aatttagttt atggtaaata cctcaaaata cctaaaatag gtacgtatca accacttaag
      721 ggtgtgaaaa ttgtttcaga ttcactgctt taaagggtct tcaaatatat tttaacagaa
      781 ccttacttca ttccaaatta ttatcagata tttcaaaatc aaaaaaaaag tatcagttct
      841 ataccaagat aaaggtgtta ctcaatacct gaagggtcac attttaaatt tattttttag
      901 tttttctttt ttgtcaacaa atgattgatg aaataatgca acacccttaa attccagaaa
      961 atggcatggt ttttccaacc tcctgtggat atggtgcatg atcaatctat tatataggat
     1021 taatacaagt tcatgctttt tgtgttatgg tgaaacaaca ttaaagaatc caatttagat
     1081 tggttgaagc acaagtataa taatccttga atgtgatcaa acctatttaa gacaccagtt
     1141 taaatccatt atcttgagac ttaggatcca acgacccaac accgtgcgtt ttattctgtc
     1201 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     1261 gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     1321 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     1381 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     1441 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     1501 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     1561 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     1621 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     1681 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     1741 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     1801 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     1861 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     1921 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     1981 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     2041 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     2101 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     2161 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     2221 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag agcttgatcc
     2281 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
     2341 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc
     2401 ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc
     2461 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
     2521 cggactggct ttctacgtga aaaggatcta ggtgaagatc ctttttgata atctcatgcc
     2581 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
     2641 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt
     2701 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt
     2761 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgccccg gcgtgtcaat
     2821 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac
     2881 cttaaactgc cgtacgtata ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2941 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     3001 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     3061 actatagggc gaattgggcc ctctagatgc atgctcgagc ggccgccagt gtgatggata
     3121 tctgcagaat tcgccctttt actcaatggt catgttgtaa agtgcactta tctctaaatt
     3181 caaagtacag tagtagacta aattggatag caggcaatat atataaagtt tacttaataa
     3241 aaatgaaaaa aattgttatt taaaagcagc ttcctggcag attaagcaca aagtagaaac
     3301 attttctctg aaattgtaac agaagtttat tctgttttaa aatatatgta tttttcattt
     3361 ttaaaatcct catttttctc ttggttttta aaaattaatt ttccatatgt cttgacatcc
     3421 tgttatgaga gaatttttct ctcatagcca taactttaaa aactcctgta ttttgtatgg
     3481 ttaatttgct gttaaaattc ttcaagagcc aaaaatcctt tcagttataa aattttcatt
     3541 cagccagatt tttgccacct tgtcttacgg gtttatacta actgtagtcc ttcccaggct
     3601 agtggtttct gcctgttttc agattttgag ttttatctgt acatttgtat gttaaagatt
     3661 gtcagaaatt gtatgagctt tcattatgat ttctgtattt aacaatacag aatttaacag
     3721 aatttctgta tttaacaatg atttctgtat ttaacaatct ttagtacagg aattttgagt
     3781 aatttgagcc aatagaatat agtactaact taattttttt tttttaatta aactttttag
     3841 taagttcttg gagacgtgct gatgacagga aagatgaccg ggtggaagag cgggaccctc
     3901 ctcgtcgagt tcctccccca gctctttcaa gagaccgaga aagagaccga gaccgagaaa
     3961 gagaaggtga aaaagagaag gcctcatgga gagctgagaa agatagggaa tctctccgtc
     4021 gtactaaaaa tgagactgat gaagacggct ggaccacagt acgacgtgga ggaggaggag
     4081 gaggatccgg tgcaggcgcc aaggagaaga gtgcttgtcc taaagatcca gccaaacctc
     4141 cggccaaggc acaagttgtg ggatggccac cggtgagatc ataccggaag aacgtgatgg
     4201 tttcctgcca aaaatcaagc ggtggcccgg aggcggcggc gttcgtgaag gtatcaatgg
     4261 acggagcacc gtacttgagg aaaatcgatt tgaggatgta taaagctagc atggtgagca
     4321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4381 acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga
     4441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4501 ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg
     4621 acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4741 acaacttcaa cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg
     4801 ctaacttcaa gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc
     4861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc
     4921 atcagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4981 tcgtgaccgc cgccgggatt acacatggca tggacgagct gtacaagtaa tctagataac
     5041 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5101 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     5281 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5341 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     5401 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5461 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     5521 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     5581 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     5641 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5701 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     5761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     5881 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5941 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6001 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6061 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6121 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6181 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     6241 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6301 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6361 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6421 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6481 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6541 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6601 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6661 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6721 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6781 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6841 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6901 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6961 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7021 ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg
     7081 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg
     7141 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7201 catgagacaa taaccctgat aaatgcttca ataatagcac gtgaggaggg ccaccatggc
     7261 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     7321 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     7381 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7441 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7501 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7561 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7621 gcaggactga cacgtgctaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7681 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7741 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8521 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcg
//
