LOCUS       sequence-381194-        8802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of S.pombe Fml1 for recombinant protein 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Hodson C, van Twest S, Dylewska M, O'Rourke JJ, Tan W, Murphy VJ, 
            Walia M, Abbouche L, Nieminuszczy J, Dunn E, Bythell-Douglas R, 
            Heierhorst J, Niedzwiedz W, Deans AJ
  TITLE     Branchpoint translocation by fork remodelers as a general mechanism 
            of R-loop removal.
  JOURNAL   Cell Rep. 2022 Dec 6;41(10):111749. doi: 
            10.1016/j.celrep.2022.111749.
  PUBMED    36476850
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-12-6"; volume: "41(10):111749. doi"; pages: " 
            10.1016/j.celrep.2022.111749"
COMMENT     Sequence Label: pFL-EGFP-3xFlag-Fml1
FEATURES             Location/Qualifiers
     source          1..8802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      105..559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(192..211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     401..422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        585..689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(690..758,759..1550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(908..927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1721..2309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2210..2229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2463..2480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2614..2838
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2905..3438)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3627..3632,3633..3649,3650..3655))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(4051..4084)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4274..4322
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4347..4366
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(4443..5153,5154..5156,5157..5159))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4443..5153,5154..5156,5157..5159))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4485..4506)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4834..4853
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5093..5114
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5156..5165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5193..5302)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5347..5438
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5363..5380
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5523..5588
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    5595..5619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8127..8151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8314..8448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8351..8370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8405..8424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8558..8723
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat
       61 tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
      121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      361 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
      421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      541 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      601 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      661 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      721 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      781 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      841 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      901 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      961 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1021 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1081 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1141 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1201 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1261 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1321 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1381 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1441 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1501 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1561 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1621 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1681 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1741 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1801 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1861 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1921 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1981 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2041 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2101 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2161 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2221 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2281 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2341 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2401 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2461 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2521 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     2581 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     2641 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     2701 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     2761 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     2821 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     2881 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     2941 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     3001 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     3061 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     3121 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     3181 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     3241 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     3301 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     3361 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     3421 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     3481 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     3541 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3601 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     3661 tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     3721 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     3781 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
     3841 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     3901 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     3961 gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga
     4021 tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag
     4081 ttatctggtt taaacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct
     4141 gcgagccctg ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg
     4201 gcgtattggt cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
     4261 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
     4321 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
     4381 tctcccggta ccgcatgcta tgcatcagct gctagcacca tggctcgaga tctgagtccg
     4441 gacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     4501 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     4561 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     4621 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     4681 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     4741 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     4801 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     4861 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     4921 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     4981 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     5041 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     5101 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     5161 gtggcgaccg ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta
     5221 tagtatttta attaatatac aaatgatttg ataataattc ttatttaact ataatatatt
     5281 gtgttgggtt gaattaaagg tccgtatact agtatcgatt cgcgacctac tccggaatat
     5341 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     5401 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     5461 ccaccatcgg gcgcggatcc cggtccgaag cgcgcggaat tcaaaggaaa cttaagctta
     5521 tggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     5581 atgacaagga tatcacaagt ttgtacaaaa aagcaggctt catgtccgat gattctttta
     5641 gtagtgatga agactgggac gaattggaca cccaagtagt tgataaaatt gagaatgaat
     5701 atcataataa cacgataggt ttaaatggat actcagttga tgaatatttt gatgccaacg
     5761 attccaatag atatcgtcta caacacgagt tagatgaaag tgcagcacag caatgggtat
     5821 accctataaa cgtctccttt cgagattatc agtttaatat tgtccaaaaa gctttatttg
     5881 aaaatgtact tgtagctttg ccaacaggtt taggtaaaac atttattgca gccgttgtga
     5941 tgatgaatta tcttcgttgg tttccaaaaa gttacattgt tttcatggct cctactaaac
     6001 cattggttac acaacaaatg gaagcttgct ataaaatcac tggaattcca aagagtcaaa
     6061 ctgctgaatt aagtggacac gttcctgtta caactcgaaa tcagtattat caaagtcgaa
     6121 atgttttctt cgtaactcct caaaccattt taaacgacat caagcatgga atttgcgatc
     6181 ggactcgtat atcttgttta gtaattgatg aagcacatcg tagtactgga aattacgctt
     6241 atgtggaagt tgttcatcta ctttctctta gcaacaaaaa tttccgtata ctggctctta
     6301 gtgctacccc cggaaataag ttagaagcaa ttcagaatgt aattgattct cttcatatat
     6361 ccaggattga gattagaact gaaaacagca ttgatatatc gcaatacgtt cagaagaaag
     6421 aggtggattt ttttcctgta gatttgtctg cagagattac agatataaga gatcgatttt
     6481 cttctatatt ggaacctatg cttcaaaaat tgaacaaggg taattattat cgaattcaga
     6541 atgctaaaga cataacttct ttcactgtgg ttcaagctaa acaagctttt cttgcaatgt
     6601 ctggtcaaaa ctttcctgct aatcaaaaat gggatatttt aaatactttt gatgcattag
     6661 cgacttttgc ctatccttta aatttgttac tcaaccatgg tatccgaccg ttttatcaaa
     6721 aattgcgaga ggtagaagaa gaatgctttg taggaagatc aggttataag aaacgcataa
     6781 taaatcacga aaattatcgt cctttaatgg acgatataga aattttgttg cgagatcagt
     6841 cttttgtcgg acatcccaaa ctagagcatt tggaacgtat tgttacagaa tattttgaaa
     6901 aagaacaaac caaggatacc agaattatga tttttgttga aatccgctct agcgccgaag
     6961 agattttacg atttttgggt aaattttatc caaatgtgag accagctatt tttattggac
     7021 aatctgctgt gagaaaggct gctggaatgt ctcaaaaatt acagaatgaa actgtgaaac
     7081 aatttcaaaa aggtgaagtc aatactttaa tagctacctc tattggagaa gagggtttgg
     7141 acataggtga agtagacatg atcatatgtt acgatgcatc tgcttctcct ataaggatgt
     7201 tacaaagaat gggcagaact gggagaaaaa ggaaaggtta tatttatatg cttttgacgc
     7261 gaggcaaaga agaagcaaaa tgggagagag ccaaagacgc ttatcgaact ttgcaagata
     7321 atattgtcag tggtcgcggt ctcagcctat cggaaaaaag ttatagaatt ttaccagaaa
     7381 agtttcgccc ggtttgtgat aaacgagtca tagaaattcc caaagaaaat gaagaagttg
     7441 tagtagctcc aaagaaagtg caattgagaa cgaaaataaa aaagaaattt ttcatgccag
     7501 aaaatgcctt gaatggattt attactgcgt ccgctttagg taaaccgaaa cgtgctttag
     7561 ccaagtctga agaatcaccg ttcgagatat gtcccgttac atatagcatt gaacaagaaa
     7621 agaaactgga aaaatataag cgtgtttgct taagaggttt ggatattcat agaaatagga
     7681 ggttatctca attgagtgtt acaggacgga taccgcattc tctagctacg aaaagtatac
     7741 attcattttt aaagcattta aatactatag attcgcaaaa ggctcaggag tggagaagag
     7801 aaataaacaa ccaattccaa gtatcaaata tcaactcgac ggatcgtgac acaaaacagc
     7861 caaagatgca tgactttaga caacctttac acccaaatcc gatgacaact ttgaaaagga
     7921 aaggacaaca taattcattt tcttacgaca agtctacact gttttatgac aacaacaata
     7981 atttagagga agacctcccg gatgtaaata tttctatatc ttcaaggaat gaagaagcat
     8041 ccaagacaaa accatttgac gaccgacaac aacgccttca gcaactcgta gagaaaagga
     8101 aacgtatgaa aggaatgctg atttagaccc agctttcttg tacaaagtgg tgatatcctc
     8161 gagtctagag cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc
     8221 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8281 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     8461 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     8521 cgctgagcaa taactatcat aacccctagg agatccgaac cagataagtg aaatctagtt
     8581 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     8641 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     8701 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa
     8761 catcctgcca actccatgtg acaaaccgtc atcttcggct ac
//
