LOCUS       sequence-381199-        8320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of human Senataxin helicase domain for 
            protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Hodson C, van Twest S, Dylewska M, O'Rourke JJ, Tan W, Murphy VJ, 
            Walia M, Abbouche L, Nieminuszczy J, Dunn E, Bythell-Douglas R, 
            Heierhorst J, Niedzwiedz W, Deans AJ
  TITLE     Branchpoint translocation by fork remodelers as a general mechanism 
            of R-loop removal.
  JOURNAL   Cell Rep. 2022 Dec 6;41(10):111749. doi: 
            10.1016/j.celrep.2022.111749.
  PUBMED    36476850
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-12-6"; volume: "41(10):111749. doi"; pages: " 
            10.1016/j.celrep.2022.111749"
COMMENT     Sequence Label: pADC10-SETXhel
FEATURES             Location/Qualifiers
     source          1..8320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(526..531,532..548,549..554))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    1062..1110
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1135..1154
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        1241..1543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1544..1747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1698..1718
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1744..1768
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1929..2038
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     5'UTR           2074..2119
                     /label=polyhedrin 5' UTR
                     /note="5' UTR from the Malacosoma neustria 
                     nucleopolyhedrovirus polyhedrin gene (Ezure et al., 2006)"
     CDS             2132..2155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4812..4946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4849..4868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4903..4922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4975..5140
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5324..5778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5411..5430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5620..5641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5804..5908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5909..5977,5978..6769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6127..6146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6940..7528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7429..7448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7682..7699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7833..8057
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(8124..8320,1..337))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
       61 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
      121 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
      181 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
      241 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
      301 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
      361 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
      421 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
      481 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
      541 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
      601 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
      661 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
      721 tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg gcgcttgccg
      781 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
      841 ttgttcgccc aggactctag ctatagttct agtggttggc tacgtacccg tagtggctat
      901 ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
      961 ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
     1021 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     1081 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     1141 ccttccgtgt ttcagttagc ctcccccatc tcccggtacc tagttattaa tagtaatcaa
     1201 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1261 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1321 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1381 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1441 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1501 ctacttggca gtacatctag tattagtcat cgctattacc atggtgatgc ggttttggca
     1561 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     1621 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     1681 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     1741 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1801 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
     1861 gattccccgt gccaagagtg acgtaagtac cgcctataga ctctataggc acaccccttt
     1921 ggctcttgga cctttaattc aacccaacac aatatattat agttaaataa gaattattat
     1981 caaatcattt gtatattaat taaaatacta tactgtaaat tacattttat ttacaatcag
     2041 acagactgtt cctttcctgg gtcttttctg cagagtattt ttattctttc gtaaaaaaat
     2101 tagaaaaata aaatataaat ctagaaccat ggactacaag gatgacgatg acaagggtgg
     2161 agtatacggg ccccctgcaa gtctttgtca gtccatctca agacctgtgc ctgtcagatt
     2221 tcacaattat ggagattatt ttaatgtttt tttccctttg atggtattga atacttttga
     2281 aacagtggca caagaatggc tcaactctcc aaatagagag aatttctatc agttgcaagt
     2341 acgaaaattt cctgccgatt atataaaata ctgggagttt gcagtttatc tggaagaatg
     2401 tgaactggct aaacagcttt atccaaagga aaacgatttg gtgtttttag ctcctgagag
     2461 aataaatgaa gagaagaaag atacaaagag aaatgacata caagatctcc acgaatatca
     2521 ttctggttat gttcataaat ttcgccgcac gtcagtcatg cgtaatggga aaactgagtg
     2581 ttacctttcc atccagactc aagagaactt tccggccaat ttaaacgaac ttgtgaattg
     2641 tattgtaatc agttctctgg taactacaca aaggaagttg aaagccatgt ctctgttggg
     2701 tagtcggaac caactggcta gagctgttct gaatccaaac cctatggact tctgtacaaa
     2761 agatttactg actacaacat ctgagagaat tattgcgtac ttaagagatt tcaatgaaga
     2821 tcaaaagaaa gcaatagaaa ctgcatatgc tatggtgaaa cactcaccat cagttgccaa
     2881 aatctgcttg attcatggac cacctggaac aggaaaatca aaaactattg ttggcctcct
     2941 ctatcgtcta ctgacagaga accagaggaa ggggcattca gacgaaaact ccaatgccaa
     3001 aatcaaacaa aaccgtgtcc tcgtgtgtgc accttccaat gcagctgttg atgaactcat
     3061 gaaaaaaatt atccttgaat tcaaagaaaa atgtaaagac aagaagaatc ctttaggaaa
     3121 ctgtggagat ataaatttag tacgactggg tccagaaaag tctattaata gtgaggttct
     3181 aaagttcagt ttggacagcc aagtaaacca cagaatgaaa aaagagttac cttctcatgt
     3241 tcaggcgatg cataaaagaa aggaatttct agattatcag ctggatgagc tttcccggca
     3301 gcgagctcta tgccgaggtg gacgggaaat acagaggcaa gaattagatg aaaacatttc
     3361 caaagtttct aaggaaaggc aggaacttgc ttctaaaatt aaagaggttc aaggacgccc
     3421 acagaaaaca cagagtatca tcatcttaga gtcccatatc atctgctgca cgttgagcac
     3481 aagtggtggt ttactacttg agtctgcttt ccgtgggcaa gggggtgtcc ccttcagctg
     3541 tgtcattgtt gatgaggctg gacagtcttg tgaaattgag actcttactc cactcatcca
     3601 tcgctgcaat aagctcatcc tagtaggaga tcctaagcag ctccctccga cagtcatctc
     3661 tatgaaagca caggagtatg gctacgacca gtcaatgatg gctcgcttct gcagactgct
     3721 ggaagagaat gtagaacaca acatgatcag caggctgccc attctacagc tcactgttca
     3781 gtacaggatg catccagaca tatgcctctt cccttctaat tatgtttata acagaaactt
     3841 aaaaacaaat agacagacag aagccattcg atgttcatca gattggccat ttcagccata
     3901 ccttgtgttt gatgttggag atggttcaga aagacgggat aatgactcat atataaatgt
     3961 tcaagaaata aaactggtga tggaaataat taagcttatt aaagacaaaa gaaaggatgt
     4021 tagttttcga aacattggca taataactca ttacaaggcc cagaagacga tgattcagaa
     4081 ggatttggac aaagagttcg atagaaaagg accagcagaa gtagacactg tggatgcatt
     4141 ccagggtcgg cagaaggatt gtgttattgt tacgtgtgtc agagcaaata gcatccaagg
     4201 ttcaattgga ttcctggcaa gtttgcagag attgaatgtc accatcacac gagccaagta
     4261 cagcctcttc atcctcggac atttgaggac cctgatggaa aaccagcatt ggaatcagct
     4321 gattcaggat gctcagaagc gtggtgccat tattaagacc tgtgacaaaa actatagaca
     4381 tgatgcagtg aagattctga aactcaagcc tgtgctgcag agaagtctca ctcaccctcc
     4441 taccatagcc ccagaggggt ccagacccca gggtggtttg cccagcagca agctagacag
     4501 tggatttgcc aagacatctg ttgctgcttc tctataccac acaccctctg actccaagga
     4561 aattactctt actgttactt caaaggaccc tgaaagacct cctgttcatg accaacttca
     4621 ggacccacga ctgctgaaga ggatgggcat tgaggtcaaa ggaggaatat tcctttggga
     4681 tccatagtgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     4741 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4801 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4921 tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctaggaga tccgaaccag
     4981 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     5041 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     5101 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     5161 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     5221 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     5281 gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5521 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5581 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     5641 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5701 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5761 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5821 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5881 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5941 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6001 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6061 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6121 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6181 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6241 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6301 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6361 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6421 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6481 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6541 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6601 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6661 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6721 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6781 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7621 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7681 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     7741 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     7801 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     7861 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     7921 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     7981 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     8041 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     8101 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     8161 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     8221 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     8281 agcgcggtgg caatgccctg cctccggtgc tcgccggaga
//
