LOCUS       sequence-381202-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of human HLTF for recombinant protein 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Hodson C, van Twest S, Dylewska M, O'Rourke JJ, Tan W, Murphy VJ, 
            Walia M, Abbouche L, Nieminuszczy J, Dunn E, Bythell-Douglas R, 
            Heierhorst J, Niedzwiedz W, Deans AJ
  TITLE     Branchpoint translocation by fork remodelers as a general mechanism 
            of R-loop removal.
  JOURNAL   Cell Rep. 2022 Dec 6;41(10):111749. doi: 
            10.1016/j.celrep.2022.111749.
  PUBMED    36476850
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-12-6"; volume: "41(10):111749. doi"; pages: " 
            10.1016/j.celrep.2022.111749"
COMMENT     Sequence Label: pFL-EGFP-3xFlag-HLTF
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      111..565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(198..217)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     407..428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        591..695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(696..764,765..1556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(914..933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1727..2315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2216..2235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2469..2486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2620..2844
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2911..3444)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3633..3638,3639..3655,3656..3661))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(4057..4090)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4280..4328
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4353..4372
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(4449..5159,5160..5162,5163..5165))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4449..5159,5160..5162,5163..5165))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4491..4512)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4840..4859
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5099..5120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5162..5171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5199..5308)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5353..5444
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5369..5386
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5529..5594
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    5601..5625
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8653..8677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8840..8974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8877..8896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8931..8950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9084..9249
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt
       61 tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg
      121 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      181 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      241 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      301 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      361 atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc
      421 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
      481 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
      541 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
      601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     2521 ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg
     2581 ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct
     2641 taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt
     2701 gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta
     2761 aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc
     2821 aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc
     2881 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     2941 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     3001 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag
     3061 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata
     3121 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
     3181 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
     3241 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
     3301 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
     3361 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
     3421 aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     3481 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     3541 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     3601 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     3661 actgggtttg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     3721 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     3781 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     3841 ccttgcttca ggagatcggt agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     3901 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
     3961 actctagcta tagttctagt ggttggctac agctttgttt gtactatcaa caggttgaac
     4021 tgctgatcaa cagatcctct acgcggccgc ggtaccataa cttcgtatag catacattat
     4081 acgaagttat ctggtttaaa cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4141 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4201 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4261 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4321 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4381 cccccatctc ccggtaccgc atgctatgca tcagctgcta gcaccatggc tcgagatctg
     4441 agtccggact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     4501 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     4561 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     4621 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     4681 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     4741 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     4801 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     4861 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     4921 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     4981 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     5041 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     5101 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     5161 accatggtgg cgaccgggtg atcaagtctt cgtcgagtga ttgtaaataa aatgtaattt
     5221 acagtatagt attttaatta atatacaaat gatttgataa taattcttat ttaactataa
     5281 tatattgtgt tgggttgaat taaaggtccg tatactagta tcgattcgcg acctactccg
     5341 gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat
     5401 tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat
     5461 accgtcccac catcgggcgc ggatcccggt ccgaagcgcg cggaattcaa aggaaactta
     5521 agcttatgga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     5581 atgacgatga caaggatatc acaagtttgt acaaaaaagt tggcatgtcc tggatgttca
     5641 agagggatcc agtttggaag tacttgcaga ctgtccagta tggagttcat ggaaattttc
     5701 cacgcctctc atatccaact ttctttccac gttttgaatt ccaagatgtt atccctccag
     5761 atgactttct aactagtgat gaagaagtag attccgtttt atttggaagt ttgagaggtc
     5821 atgtggttgg actacgctat tacacgggag tagttaataa taatgaaatg gttgcattac
     5881 aacgagatcc taataaccct tatgataaga atgcaattaa agtaaacaat gtgaatggaa
     5941 atcaagttgg ccatttaaag aaagagcttg caggtgcttt ggcctatatc atggacaaca
     6001 aattggcaca aattgaaggg gtagttcctt ttggtgcaaa caatgctttt accatgcctc
     6061 tgcatatgac tttttgggga aaagaagaaa atagaaaagc ggtttcagat cagttgaaga
     6121 aacatggatt taaattgggt cctgcaccaa aaactttagg attcaatttg gaaagtggtt
     6181 ggggctctgg aagagctgga ccaagctata gtatgccagt gcatgctgca gtacagatga
     6241 caactgaaca gcttaaaaca gaatttgaca aattgtttga agatttaaaa gaagatgata
     6301 aaacccatga aatggaacca gctgaggcta ttgaaacacc actgcttcca catcaaaaac
     6361 aagctctagc ttggatggtg tcacgggaaa atagcaaaga acttccacca ttctgggaac
     6421 agcgaaatga cttatactat aacacaataa caaatttttc tgagaaggac cgaccagaaa
     6481 atgtccatgg aggaatttta gctgatgata tgggtttggg taaaactctt acagccattg
     6541 cagtaatcct taccaacttc catgatggca gacctcttcc tattgaaaga gttaaaaaga
     6601 atctactgaa gaaagagtac aacgtgaatg acgactctat gaaacttgga ggaaacaata
     6661 ccagtgaaaa ggcagatgga ctaagcaaag acgcatctag atgtagtgaa caacccagta
     6721 tttcagatat caaggagaag agtaagtttc gcatgtcaga attgtctagc tcccgcccca
     6781 aaagaagaaa aactgctgtc cagtacatag aaagcagtga ttcagaggaa attgaaacaa
     6841 gtgaattgcc gcagaaaatg aaaggcaaac tgaaaaatgt acagtctgaa actaaaggca
     6901 gggcgaaagg atcttctaag gttatagaag atgtggcatt tgcatgtgca ttaacttcat
     6961 ctgttcctac aacaaaaaag aaaatgttga aaaagggagc ttgtgcagtg gaggggtcaa
     7021 agaaaactga tgttgaggag agaccaagaa caacactgat catctgtccg ctttctgtgt
     7081 taagcaactg gattgaccag tttggacaac atataaaatc agatgtacac ttgaattttt
     7141 atgtttatta tggtcctgat cgtattagag aaccggcctt actttcaaaa caggatattg
     7201 ttttgactac gtataatatt ttaactcatg actatggaac taaaggagat agtccattac
     7261 atagcataag gtggctaaga gtgatcctgg atgaaggaca tgccatacga aatccaaatg
     7321 ctcagcagac aaaagctgta cttgacttag aatcagaaag aagatgggtt ttgacaggta
     7381 ctccaatcca gaattcttta aaggacttgt ggtctcttct ttccttttta aaacttaaac
     7441 catttattga tagagaatgg tggcatagaa caatacagcg tcctgtcaca atgggagatg
     7501 aaggaggact taggcgttta cagtccctaa ttaaaaatat tacacttaga agaacaaaga
     7561 caagcaaaat taaaggaaaa cctgttttgg agttaccaga acgtaaagta tttattcagc
     7621 acattacact ttcagatgaa gagagaaaga tttatcagtc tgtgaaaaat gaaggcagag
     7681 ccactattgg aaggtatttt aatgaaggga ctgtcctggc acattatgca gatgtcctgg
     7741 gtcttttgct tagactgcgg caaatttgtt gccatactta ccttcttaca aatgcagtgt
     7801 cttccaatgg cccctcagga aatgatacac ctgaagaact gagaaagaag ttaataagga
     7861 agatgaagtt aattctgagc tcaggttcag atgaggaatg tgcaatttgc ctggattctt
     7921 taacagttcc tgtgataaca cattgtgcac atgtattttg taaaccctgt atttgccaag
     7981 tcattcagaa tgagcagcca catgctaaat gccctttatg cagaaatgat atacatgaag
     8041 ataatttatt agaatgtcct ccagaagaat tagcacgtga cagtgagaaa aagtctgata
     8101 tggaatggac atccagttca aagattaatg cgctaatgca cgcattgact gacttaagaa
     8161 agaagaatcc caacataaaa agtttggttg tttctcagtt tacaacattc ctgtctttaa
     8221 tagaaatacc acttaaagcc tctggatttg tgtttactcg tttggatggt tccatggccc
     8281 aaaagaaaag agttgaatca attcagtgtt ttcaaaacac tgaagcagga tctccaacta
     8341 taatgcttct gtccttaaaa gcaggtggag ttggtttgaa tctgtctgca gcttctcgag
     8401 tgtttttaat ggatccagcc tggaatcctg ctgctgaaga tcagtgcttt gacagatgcc
     8461 atagacttgg tcagaagcaa gaagttatca tcacaaaatt cattgtaaag gactctgttg
     8521 aagaaaatat gctgaaaata caaaacaaaa agagagaact tgcagcagga gcctttggaa
     8581 ctaaaaaacc aaatgctgac gaaatgaaac aagccaaaat taatgaaatc agaacattaa
     8641 ttgacttata atacccaact ttcttgtaca aagtggtgat atcctcgagt ctagagcctg
     8701 cagtctcgac aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     8761 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8821 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8941 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
     9001 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     9061 tatcataacc cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt
     9121 catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact
     9181 cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca actattttgt
     9241 ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc
     9301 catgtgacaa accgtcatct tc
//
