LOCUS       sequence-381220-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 
            promoter driven guide RNA that target hAAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CMV:AsCpf1-2A-GFP-U6-AAVS1-sg
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          167..394
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             422..442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             467..4384
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4104..4113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4385..4432
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4448..4501
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4502..4504,4505..5215)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4502..4504,4505..5215)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4544..4565)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4805..4824)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5152..5173
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5243..5260)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5249..5456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5465..5605
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5465..5594
                     /label=AAV2 ITR
     rep_origin      5680..6135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5767..5786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5977..5998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6152..6171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6271..6293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6331..6349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6417..6521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6522..6590,6591..7382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6740..6759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7553..8141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8042..8061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8203..8451
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8203..8223
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8374..8393
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8502..8577
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8674..8959
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgrggsg
       61 grgaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      121 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
      181 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      241 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      301 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
      361 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtggccg ccaccatggc
      421 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccacac agttcgaggg
      481 ctttaccaac ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa
      541 gaccctgaag cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca
      601 ctacaaggag ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct
      661 gcagctggtg cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga
      721 gaaaaccgag gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat
      781 ccacgactac ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc
      841 cgagatctac aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct
      901 gggcaccgtg accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac
      961 aacctacttc tccggctttt atgagaacag gaagaacgtg ttcagcgccg aggatatcag
     1021 cacagccatc ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca
     1081 catcttcaca cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa
     1141 gaaggccatc ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta
     1201 taaccagctg ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc
     1261 tcgggaggca ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca
     1321 gaagaatgat gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt
     1381 taagcagatc ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga
     1441 cgaggaagtg atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct
     1501 ggagacagcc gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat
     1561 cagccacaag aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag
     1621 gaatgccctg tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa
     1681 ggagaaggtg cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc
     1741 cgcaggcaag gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc
     1801 acacgccgcc ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat
     1861 cctgaagtct cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt
     1921 ggatgagtcc aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga
     1981 gatggagcct tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta
     2041 ctccgtggag aagttcaagc tgaactttca gatgcctaca ctggcctctg gctgggacgt
     2101 gaataaggag aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg
     2161 catcatgcca aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac
     2221 cagcgagggc tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc
     2281 aaagtgcagc acccagctga aggccgtgac agcccacttt cagacccaca caacccccat
     2341 cctgctgtcc aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa
     2401 caatcctgag aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca
     2461 gaagggctac agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa
     2521 gtataccaag acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga
     2581 cctgggcgag tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat
     2641 cgccgagaag gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta
     2701 taacaaggac tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac
     2761 cggcctgttt tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga
     2821 gctgttctac cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat
     2881 gctgaacaag aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct
     2941 gtacgactat gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct
     3001 gcccaacgtg atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag
     3061 cgacaagttc tttttccacg tgcctatcac actgaactat caggccgcca attccccatc
     3121 taagttcaac cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg
     3181 catcgatcgg ggcgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat
     3241 cctggagcag cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa
     3301 cagggagaag gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga
     3361 tctgaagcag ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta
     3421 ccaggccgtg gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat
     3481 cgccgagaag gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct
     3541 ggtgctgaag gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac
     3601 agaccagttc acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc
     3661 tgccccatat acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa
     3721 aaccatcaag aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta
     3781 cgacgtgaaa accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca
     3841 gaggggcctg cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca
     3901 gtttgacgcc aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa
     3961 tcacagattc accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct
     4021 ggaggagaag ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa
     4081 tgacgattct cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg
     4141 gaactccaat gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg
     4201 cgtgtgcttc gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg
     4261 cgcctaccac atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga
     4321 tctgaagctg cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg
     4381 caacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggaattcgg
     4441 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     4501 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4561 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4621 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4681 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     4741 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4801 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4861 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4921 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4981 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5041 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5101 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5161 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaatt
     5221 ctaactagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5281 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5341 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5401 gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg
     5461 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     5521 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     5581 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     5641 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     5701 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     5761 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5821 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5881 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5941 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6001 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     6061 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6121 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6181 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6241 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6301 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6361 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6601 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6661 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6721 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6841 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6901 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6961 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7021 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7081 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7141 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7201 ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac
     7261 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7321 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7381 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7441 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7501 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7561 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7621 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7681 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7741 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7801 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7861 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7921 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7981 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8161 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgagggcct atttcccatg
     8221 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     8281 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     8341 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     8401 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtaatttct
     8461 actcttgtag atatctgtcc cctccacccc acagtttttt tgttttagag ctagaaatag
     8521 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     8581 tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg
     8641 ccaattctgc agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc
     8701 gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac
     8761 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8821 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     8881 gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     8941 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     9001 ccccccctcc cc
//
