LOCUS       sequence-381223-        9207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 
            promoter driven guide RNA that target hMAFB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CMV:AsCpf1-2A-GFP-U6-MAFB-sg
FEATURES             Location/Qualifiers
     source          1..9207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          282..509
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      525..534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             537..557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             582..4499
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4219..4228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4500..4547
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4563..4616
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4617..4619,4620..5330)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4617..4619,4620..5330)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4659..4680)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4920..4939)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5267..5288
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5358..5375)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5364..5571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5580..5720
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5580..5709
                     /label=AAV2 ITR
     rep_origin      5795..6250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5882..5901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6092..6113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6267..6286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6386..6408
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6446..6464)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6532..6636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6637..6705,6706..7497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6855..6874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7668..8256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8157..8176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8318..8566
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8318..8338
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8489..8508
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8618..8693
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8790..9075
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      121 gggggggggg gggggggggg gggggggggg gsgaggggcg gggcggggcg aggcggagag
      181 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      241 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      301 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      361 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga
      421 gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg
      481 agcacctgcc tgaaatcact ttttttcagg ttggaccggt ggccgccacc atggccccaa
      541 agaagaagcg gaaggtcggt atccacggag tcccagcagc cacacagttc gagggcttta
      601 ccaacctgta tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc
      661 tgaagcacat ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca
      721 aggagctgaa gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc
      781 tggtgcagct ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa
      841 ccgaggagac aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg
      901 actacttcat cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga
      961 tctacaaggg cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca
     1021 ccgtgaccac aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct
     1081 acttctccgg cttttatgag aacaggaaga acgtgttcag cgccgaggat atcagcacag
     1141 ccatcccaca ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct
     1201 tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg
     1261 ccatcggcat cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc
     1321 agctgctgac acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg
     1381 aggcaggcac cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga
     1441 atgatgagac agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc
     1501 agatcctgtc cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg
     1561 aagtgatcca gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga
     1621 cagccgaggc cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc
     1681 acaagaagct ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg
     1741 ccctgtatga gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga
     1801 aggtgcagcg cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag
     1861 gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg
     1921 ccgccctgga tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga
     1981 agtctcagct ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg
     2041 agtccaacga ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg
     2101 agccttctct gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg
     2161 tggagaagtt caagctgaac tttcagatgc ctacactggc ctctggctgg gacgtgaata
     2221 aggagaagaa caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca
     2281 tgccaaagca gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg
     2341 agggctttga taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt
     2401 gcagcaccca gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc
     2461 tgtccaacaa tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc
     2521 ctgagaagga gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg
     2581 gctacagaga ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata
     2641 ccaagacaac ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg
     2701 gcgagtacta tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg
     2761 agaaggagat catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca
     2821 aggactttgc caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc
     2881 tgttttctcc agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt
     2941 tctaccgccc taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga
     3001 acaagaagct gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg
     3061 actatgtgaa tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca
     3121 acgtgatcac caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca
     3181 agttcttttt ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt
     3241 tcaaccagag ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg
     3301 atcggggcga gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg
     3361 agcagcggag cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg
     3421 agaaggagag ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga
     3481 agcagggcta tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg
     3541 ccgtggtggt gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg
     3601 agaaggccgt gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc
     3661 tgaaggacta tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc
     3721 agttcacctc ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc
     3781 catatacatc taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca
     3841 tcaagaatca cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg
     3901 tgaaaaccgg cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg
     3961 gcctgcccgg ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg
     4021 acgccaaggg cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca
     4081 gattcaccgg cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg
     4141 agaagggcat cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg
     4201 attctcacgc catcgacacc atggtggccc tgatccgcag cgtgctgcag atgcggaact
     4261 ccaatgccgc cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt
     4321 gcttcgactc ccggtttcag aacccagagt ggcccatgga cgccgatgcc aatggcgcct
     4381 accacatcgc cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga
     4441 agctgcagaa cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca
     4501 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggaa ttcggcagtg
     4561 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga
     4621 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4681 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4741 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4801 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4861 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4921 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4981 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5041 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     5101 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     5161 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5221 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5281 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gaattctaac
     5341 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5401 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5461 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5521 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca
     5581 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     5641 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     5701 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5761 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     5821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     5881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6001 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6121 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     6181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6241 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6301 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6361 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6421 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6481 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6601 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6661 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6721 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6781 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6841 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6901 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6961 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7021 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7081 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7141 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7201 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7261 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7321 attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg
     7381 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7441 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7501 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7561 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7741 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7801 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7861 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7921 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7981 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8041 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8101 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8161 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8221 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8281 cggttcctgg ccttttgctg gccttttgct cacatgtgag ggcctatttc ccatgattcc
     8341 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
     8401 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     8461 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     8521 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgtaa tttctactct
     8581 tgtagattgt gagtcgtggc cggtcctggc cttttttgtt ttagagctag aaatagcaag
     8641 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
     8701 ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa
     8761 ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct
     8821 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
     8881 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     8941 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9001 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9061 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     9121 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     9181 gcgggggggg gggggggggs sscmsgc
//
