LOCUS       sequence-381230-       10774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 
            159281), with guide RNA that target hAAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
FEATURES             Location/Qualifiers
     source          1..10774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          112..1128
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1052..1074
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1136..1155
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1195..1215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1240..5157
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4877..4886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5158..5205
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5212..5292
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5302..5355
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5356..5358,5359..6069)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5356..5358,5359..6069)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5398..5419)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5659..5678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6006..6027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6097..6114)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6103..6310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6319..6459
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6319..6448
                     /label=AAV2 ITR
     rep_origin      6534..6989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6621..6640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6831..6852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7006..7025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7125..7147
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7185..7203)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7271..7375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7376..7444,7445..8236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7594..7613)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8407..8995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8896..8915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9057..9305
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9057..9077
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9228..9247
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9356..9431
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     9655..9674
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9795..10256
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        10354..10657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
       61 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      121 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      181 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      241 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
      301 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
      361 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
      421 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
      481 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
      541 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
      601 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
      661 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      721 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
      781 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
      841 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
      901 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
      961 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1021 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1081 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1141 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagacc ggtccaccat ggccccaaag
     1201 aagaagcgga aggtcggtat ccacggagtc ccagcagcca cacagttcga gggctttacc
     1261 aacctgtatc aggtgagcaa gacactgcgg tttgagctga tcccacaggg caagaccctg
     1321 aagcacatcc aggagcaggg cttcatcgag gaggacaagg cccgcaatga tcactacaag
     1381 gagctgaagc ccatcatcga tcggatctac aagacctatg ccgaccagtg cctgcagctg
     1441 gtgcagctgg attgggagaa cctgagcgcc gccatcgact cctatagaaa ggagaaaacc
     1501 gaggagacaa ggaacgccct gatcgaggag caggccacat atcgcaatgc catccacgac
     1561 tacttcatcg gccggacaga caacctgacc gatgccatca ataagagaca cgccgagatc
     1621 tacaagggcc tgttcaaggc cgagctgttt aatggcaagg tgctgaagca gctgggcacc
     1681 gtgaccacaa ccgagcacga gaacgccctg ctgcggagct tcgacaagtt tacaacctac
     1741 ttctccggct tttatgagaa caggaagaac gtgttcagcg ccgaggatat cagcacagcc
     1801 atcccacacc gcatcgtgca ggacaacttc cccaagttta aggagaattg tcacatcttc
     1861 acacgcctga tcaccgccgt gcccagcctg cgggagcact ttgagaacgt gaagaaggcc
     1921 atcggcatct tcgtgagcac ctccatcgag gaggtgtttt ccttcccttt ttataaccag
     1981 ctgctgacac agacccagat cgacctgtat aaccagctgc tgggaggaat ctctcgggag
     2041 gcaggcaccg agaagatcaa gggcctgaac gaggtgctga atctggccat ccagaagaat
     2101 gatgagacag cccacatcat cgcctccctg ccacacagat tcatccccct gtttaagcag
     2161 atcctgtccg ataggaacac cctgtctttc atcctggagg agtttaagag cgacgaggaa
     2221 gtgatccagt ccttctgcaa gtacaagaca ctgctgagaa acgagaacgt gctggagaca
     2281 gccgaggccc tgtttaacga gctgaacagc atcgacctga cacacatctt catcagccac
     2341 aagaagctgg agacaatcag cagcgccctg tgcgaccact gggatacact gaggaatgcc
     2401 ctgtatgagc ggagaatctc cgagctgaca ggcaagatca ccaagtctgc caaggagaag
     2461 gtgcagcgca gcctgaagca cgaggatatc aacctgcagg agatcatctc tgccgcaggc
     2521 aaggagctga gcgaggcctt caagcagaaa accagcgaga tcctgtccca cgcacacgcc
     2581 gccctggatc agccactgcc tacaaccctg aagaagcagg aggagaagga gatcctgaag
     2641 tctcagctgg acagcctgct gggcctgtac cacctgctgg actggtttgc cgtggatgag
     2701 tccaacgagg tggaccccga gttctctgcc cggctgaccg gcatcaagct ggagatggag
     2761 ccttctctga gcttctacaa caaggccaga aattatgcca ccaagaagcc ctactccgtg
     2821 gagaagttca agctgaactt tcagatgcct acactggcct ctggctggga cgtgaataag
     2881 gagaagaaca atggcgccat cctgtttgtg aagaacggcc tgtactatct gggcatcatg
     2941 ccaaagcaga agggcaggta taaggccctg agcttcgagc ccacagagaa aaccagcgag
     3001 ggctttgata agatgtacta tgactacttc cctgatgccg ccaagatgat cccaaagtgc
     3061 agcacccagc tgaaggccgt gacagcccac tttcagaccc acacaacccc catcctgctg
     3121 tccaacaatt tcatcgagcc tctggagatc acaaaggaga tctacgacct gaacaatcct
     3181 gagaaggagc caaagaagtt tcagacagcc tacgccaaga aaaccggcga ccagaagggc
     3241 tacagagagg ccctgtgcaa gtggatcgac ttcacaaggg attttctgtc caagtatacc
     3301 aagacaacct ctatcgatct gtctagcctg cggccatcct ctcagtataa ggacctgggc
     3361 gagtactatg ccgagctgaa tcccctgctg taccacatca gcttccagag aatcgccgag
     3421 aaggagatca tggatgccgt ggagacaggc aagctgtacc tgttccagat ctataacaag
     3481 gactttgcca agggccacca cggcaagcct aatctgcaca cactgtattg gaccggcctg
     3541 ttttctccag agaacctggc caagacaagc atcaagctga atggccaggc cgagctgttc
     3601 taccgcccta agtccaggat gaagaggatg gcacaccggc tgggagagaa gatgctgaac
     3661 aagaagctga aggatcagaa aaccccaatc cccgacaccc tgtaccagga gctgtacgac
     3721 tatgtgaatc acagactgtc ccacgacctg tctgatgagg ccagggccct gctgcccaac
     3781 gtgatcacca aggaggtgtc tcacgagatc atcaaggata ggcgctttac cagcgacaag
     3841 ttctttttcc acgtgcctat cacactgaac tatcaggccg ccaattcccc atctaagttc
     3901 aaccagaggg tgaatgccta cctgaaggag caccccgaga cacctatcat cggcatcgat
     3961 cggggcgaga gaaacctgat ctatatcaca gtgatcgact ccaccggcaa gatcctggag
     4021 cagcggagcc tgaacaccat ccagcagttt gattaccaga agaagctgga caacagggag
     4081 aaggagaggg tggcagcaag gcaggcctgg tctgtggtgg gcacaatcaa ggatctgaag
     4141 cagggctatc tgagccaggt catccacgag atcgtggacc tgatgatcca ctaccaggcc
     4201 gtggtggtgc tggagaacct gaatttcggc tttaagagca agaggaccgg catcgccgag
     4261 aaggccgtgt accagcagtt cgagaagatg ctgatcgata agctgaattg cctggtgctg
     4321 aaggactatc cagcagagaa agtgggaggc gtgctgaacc cataccagct gacagaccag
     4381 ttcacctcct ttgccaagat gggcacccag tctggcttcc tgttttacgt gcctgcccca
     4441 tatacatcta agatcgatcc cctgaccggc ttcgtggacc ccttcgtgtg gaaaaccatc
     4501 aagaatcacg agagccgcaa gcacttcctg gagggcttcg actttctgca ctacgacgtg
     4561 aaaaccggcg acttcatcct gcactttaag atgaacagaa atctgtcctt ccagaggggc
     4621 ctgcccggct ttatgcctgc atgggatatc gtgttcgaga agaacgagac acagtttgac
     4681 gccaagggca cccctttcat cgccggcaag agaatcgtgc cagtgatcga gaatcacaga
     4741 ttcaccggca gataccggga cctgtatcct gccaacgagc tgatcgccct gctggaggag
     4801 aagggcatcg tgttcaggga tggctccaac atcctgccaa agctgctgga gaatgacgat
     4861 tctcacgcca tcgacaccat ggtggccctg atccgcagcg tgctgcagat gcggaactcc
     4921 aatgccgcca caggcgagga ctatatcaac agccccgtgc gcgatctgaa tggcgtgtgc
     4981 ttcgactccc ggtttcagaa cccagagtgg cccatggacg ccgatgccaa tggcgcctac
     5041 cacatcgccc tgaagggcca gctgctgctg aatcacctga aggagagcaa ggatctgaag
     5101 ctgcagaacg gcatctccaa tcaggactgg ctggcctaca tccaggagct gcgcaacaaa
     5161 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac
     5221 gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc
     5281 cccgactatg ccggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     5341 gagaatcctg gcccagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     5401 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     5461 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     5521 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     5581 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     5641 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     5701 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     5761 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     5821 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     5881 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5941 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     6001 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     6061 ctgtacaagg aattctaact agagctcgct gatcagcctc gactgtgcct tctagttgcc
     6121 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6181 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6241 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc
     6301 atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     6361 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     6421 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     6481 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     6541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6601 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6721 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     6781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6841 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     6901 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6961 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     7021 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7081 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7141 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     7201 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7261 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7321 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7381 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7441 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7501 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7561 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7621 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7681 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7741 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7801 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7861 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7921 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7981 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8041 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg
     8101 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8161 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8221 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8281 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8341 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8401 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8461 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8521 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8581 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8641 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8701 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8761 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8821 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8881 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8941 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9001 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtgagg
     9061 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     9121 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     9181 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     9241 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     9301 acaccgtaat ttctactctt gtagatatct gtcccctcca ccccacagtt tttttgtttt
     9361 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     9421 cgagtcggtg cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     9481 ttttagcgcg tgcgccaatt ctgcagacaa atggctctag gcatgccgac ggcgaggatc
     9541 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     9601 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     9661 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9721 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     9781 tctgagggga tccgctgtaa gtctgcagaa attgatgatc tattaaacaa taaagatgtc
     9841 cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta
     9901 cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc
     9961 ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc
    10021 ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat
    10081 agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta
    10141 agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct
    10201 gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg
    10261 ggagctccac cgcggtggcg gccgctctag aactagttat taatagtaat caattacggg
    10321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    10381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    10441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    10501 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    10561 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
    10621 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
    10681 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
    10741 attattttgt gcagcgatgg gggcgggggg gggg
//
