LOCUS       sequence-381234-       11224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 
            159281), with guide RNA array targeting both AAVS1 and MAFB .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
FEATURES             Location/Qualifiers
     source          1..11224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          350..1366
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1290..1312
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1374..1393
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1433..1453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1478..5395
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      5115..5124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5396..5443
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5450..5530
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5540..5593
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5594..5596,5597..6307)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5594..5596,5597..6307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5636..5657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5897..5916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6244..6265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6335..6352)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6341..6548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6557..6697
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6557..6686
                     /label=AAV2 ITR
     rep_origin      6772..7227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6859..6878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7069..7090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7244..7263)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7363..7385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7423..7441)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7509..7613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7614..7682,7683..8474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7832..7851)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8645..9233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9134..9153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9295..9543
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9295..9315
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9466..9485
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9637..9712
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     9936..9955
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10076..10537
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        10635..10938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccccccccc cccccccccc ccccnnccyc cccccccccc cccccccccc cccccccccc
       61 cccccccccc cccccccccc cccccccccc cccccccccc cccccccmcc yccccccccy
      121 ccccccccmy wwtttttttt tttttttttt tnntttwttt ggtggggggg gggggggggg
      181 gggggggggg gggcgcgcgc cggggggggc ggggcggggc gaggggcggg gcggggcgag
      241 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
      301 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      361 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      421 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      481 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      541 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      601 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      661 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      721 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      781 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      841 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      901 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      961 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     1021 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     1081 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     1141 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     1201 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1261 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1321 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1381 gctggttatt gtgctgtctc atcattttgg caaagaccgg tccaccatgg ccccaaagaa
     1441 gaagcggaag gtcggtatcc acggagtccc agcagccaca cagttcgagg gctttaccaa
     1501 cctgtatcag gtgagcaaga cactgcggtt tgagctgatc ccacagggca agaccctgaa
     1561 gcacatccag gagcagggct tcatcgagga ggacaaggcc cgcaatgatc actacaagga
     1621 gctgaagccc atcatcgatc ggatctacaa gacctatgcc gaccagtgcc tgcagctggt
     1681 gcagctggat tgggagaacc tgagcgccgc catcgactcc tatagaaagg agaaaaccga
     1741 ggagacaagg aacgccctga tcgaggagca ggccacatat cgcaatgcca tccacgacta
     1801 cttcatcggc cggacagaca acctgaccga tgccatcaat aagagacacg ccgagatcta
     1861 caagggcctg ttcaaggccg agctgtttaa tggcaaggtg ctgaagcagc tgggcaccgt
     1921 gaccacaacc gagcacgaga acgccctgct gcggagcttc gacaagttta caacctactt
     1981 ctccggcttt tatgagaaca ggaagaacgt gttcagcgcc gaggatatca gcacagccat
     2041 cccacaccgc atcgtgcagg acaacttccc caagtttaag gagaattgtc acatcttcac
     2101 acgcctgatc accgccgtgc ccagcctgcg ggagcacttt gagaacgtga agaaggccat
     2161 cggcatcttc gtgagcacct ccatcgagga ggtgttttcc ttcccttttt ataaccagct
     2221 gctgacacag acccagatcg acctgtataa ccagctgctg ggaggaatct ctcgggaggc
     2281 aggcaccgag aagatcaagg gcctgaacga ggtgctgaat ctggccatcc agaagaatga
     2341 tgagacagcc cacatcatcg cctccctgcc acacagattc atccccctgt ttaagcagat
     2401 cctgtccgat aggaacaccc tgtctttcat cctggaggag tttaagagcg acgaggaagt
     2461 gatccagtcc ttctgcaagt acaagacact gctgagaaac gagaacgtgc tggagacagc
     2521 cgaggccctg tttaacgagc tgaacagcat cgacctgaca cacatcttca tcagccacaa
     2581 gaagctggag acaatcagca gcgccctgtg cgaccactgg gatacactga ggaatgccct
     2641 gtatgagcgg agaatctccg agctgacagg caagatcacc aagtctgcca aggagaaggt
     2701 gcagcgcagc ctgaagcacg aggatatcaa cctgcaggag atcatctctg ccgcaggcaa
     2761 ggagctgagc gaggccttca agcagaaaac cagcgagatc ctgtcccacg cacacgccgc
     2821 cctggatcag ccactgccta caaccctgaa gaagcaggag gagaaggaga tcctgaagtc
     2881 tcagctggac agcctgctgg gcctgtacca cctgctggac tggtttgccg tggatgagtc
     2941 caacgaggtg gaccccgagt tctctgcccg gctgaccggc atcaagctgg agatggagcc
     3001 ttctctgagc ttctacaaca aggccagaaa ttatgccacc aagaagccct actccgtgga
     3061 gaagttcaag ctgaactttc agatgcctac actggcctct ggctgggacg tgaataagga
     3121 gaagaacaat ggcgccatcc tgtttgtgaa gaacggcctg tactatctgg gcatcatgcc
     3181 aaagcagaag ggcaggtata aggccctgag cttcgagccc acagagaaaa ccagcgaggg
     3241 ctttgataag atgtactatg actacttccc tgatgccgcc aagatgatcc caaagtgcag
     3301 cacccagctg aaggccgtga cagcccactt tcagacccac acaaccccca tcctgctgtc
     3361 caacaatttc atcgagcctc tggagatcac aaaggagatc tacgacctga acaatcctga
     3421 gaaggagcca aagaagtttc agacagccta cgccaagaaa accggcgacc agaagggcta
     3481 cagagaggcc ctgtgcaagt ggatcgactt cacaagggat tttctgtcca agtataccaa
     3541 gacaacctct atcgatctgt ctagcctgcg gccatcctct cagtataagg acctgggcga
     3601 gtactatgcc gagctgaatc ccctgctgta ccacatcagc ttccagagaa tcgccgagaa
     3661 ggagatcatg gatgccgtgg agacaggcaa gctgtacctg ttccagatct ataacaagga
     3721 ctttgccaag ggccaccacg gcaagcctaa tctgcacaca ctgtattgga ccggcctgtt
     3781 ttctccagag aacctggcca agacaagcat caagctgaat ggccaggccg agctgttcta
     3841 ccgccctaag tccaggatga agaggatggc acaccggctg ggagagaaga tgctgaacaa
     3901 gaagctgaag gatcagaaaa ccccaatccc cgacaccctg taccaggagc tgtacgacta
     3961 tgtgaatcac agactgtccc acgacctgtc tgatgaggcc agggccctgc tgcccaacgt
     4021 gatcaccaag gaggtgtctc acgagatcat caaggatagg cgctttacca gcgacaagtt
     4081 ctttttccac gtgcctatca cactgaacta tcaggccgcc aattccccat ctaagttcaa
     4141 ccagagggtg aatgcctacc tgaaggagca ccccgagaca cctatcatcg gcatcgatcg
     4201 gggcgagaga aacctgatct atatcacagt gatcgactcc accggcaaga tcctggagca
     4261 gcggagcctg aacaccatcc agcagtttga ttaccagaag aagctggaca acagggagaa
     4321 ggagagggtg gcagcaaggc aggcctggtc tgtggtgggc acaatcaagg atctgaagca
     4381 gggctatctg agccaggtca tccacgagat cgtggacctg atgatccact accaggccgt
     4441 ggtggtgctg gagaacctga atttcggctt taagagcaag aggaccggca tcgccgagaa
     4501 ggccgtgtac cagcagttcg agaagatgct gatcgataag ctgaattgcc tggtgctgaa
     4561 ggactatcca gcagagaaag tgggaggcgt gctgaaccca taccagctga cagaccagtt
     4621 cacctccttt gccaagatgg gcacccagtc tggcttcctg ttttacgtgc ctgccccata
     4681 tacatctaag atcgatcccc tgaccggctt cgtggacccc ttcgtgtgga aaaccatcaa
     4741 gaatcacgag agccgcaagc acttcctgga gggcttcgac tttctgcact acgacgtgaa
     4801 aaccggcgac ttcatcctgc actttaagat gaacagaaat ctgtccttcc agaggggcct
     4861 gcccggcttt atgcctgcat gggatatcgt gttcgagaag aacgagacac agtttgacgc
     4921 caagggcacc cctttcatcg ccggcaagag aatcgtgcca gtgatcgaga atcacagatt
     4981 caccggcaga taccgggacc tgtatcctgc caacgagctg atcgccctgc tggaggagaa
     5041 gggcatcgtg ttcagggatg gctccaacat cctgccaaag ctgctggaga atgacgattc
     5101 tcacgccatc gacaccatgg tggccctgat ccgcagcgtg ctgcagatgc ggaactccaa
     5161 tgccgccaca ggcgaggact atatcaacag ccccgtgcgc gatctgaatg gcgtgtgctt
     5221 cgactcccgg tttcagaacc cagagtggcc catggacgcc gatgccaatg gcgcctacca
     5281 catcgccctg aagggccagc tgctgctgaa tcacctgaag gagagcaagg atctgaagct
     5341 gcagaacggc atctccaatc aggactggct ggcctacatc caggagctgc gcaacaaaag
     5401 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct acccatacga
     5461 tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat atgatgtccc
     5521 cgactatgcc ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     5581 gaatcctggc ccagtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5641 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     5701 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     5761 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     5821 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5881 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5941 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6001 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     6061 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     6121 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6181 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6241 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6301 gtacaaggaa ttctaactag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     6361 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6421 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6481 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat
     6541 gctggggagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     6601 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     6661 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     6721 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     6781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6841 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6961 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     7021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7081 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     7141 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7201 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     7261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     8341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgagggc
     9301 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     9361 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     9421 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     9481 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     9541 accgtaattt ctactcttgt agatatctgt cccctccacc ccacagttaa tttctactct
     9601 tgtagattgt gagtcgtggc cggtcctggc ttttttgttt tagagctaga aatagcaagt
     9661 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
     9721 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
     9781 tctgcagaca aatggctcta ggcatgccga cggcgaggat ctcgtcgtga cccatggcga
     9841 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     9901 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9961 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10021 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg atccgctgta
    10081 agtctgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt
    10141 cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt
    10201 ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct
    10261 ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa
    10321 ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt
    10381 gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat
    10441 gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt
    10501 ctcagtattg ttttgccaag ttctaattcc atcagaagct gggagctcca ccgcggtggc
    10561 ggccgctcta gaactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
    10621 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
    10681 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
    10741 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
    10801 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
    10861 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
    10921 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
    10981 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
    11041 ggggcggggg gggggggggg gcgsgcsccg gggggggggg gggggggggg gggggggggg
    11101 gggggggggg gggggggggg gggggggggg gngggggggg gggggggggg gggngggggg
    11161 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    11221 gggg
//
