LOCUS       sequence-381236-        8306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mClover-NeoR to C-terminus of 
            endogenous human eIF3f.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3f- pMK289 (mAID-mClover-NeoR) plasmid
FEATURES             Location/Qualifiers
     source          1..8306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    86..107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(122..127,128..145,146..152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    160..176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     165..187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     184..200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     184..200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        218..236
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     218..235
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1124..1327
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(1334..1336,1337..1339,1340..2053)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1379..1400)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1987..2008
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2171..2292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2208..2227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2262..2281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2299..2754)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2436..2457)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2648..2667
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2781..2885
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2887..3244
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2887..3078
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2910..2930)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3095..3230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3279..4073
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3279..4073
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4261..4280)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(5173..5192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5174..5192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5199..5216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5199..5215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5208..5230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5353..5655
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5534..5553
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6004..6798
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6004..6798
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      6998..7007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7004..7378
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7516..8104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8005..8024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8258..8275
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
       61 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      181 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
      241 tcaagctatg catcaagctt ggtaccacta gtaacggccg ccagtgtgct ggaattcgcc
      301 cttagtcttt gttttggtac tcagtctgga aaggtgtcag ctgacaatac tgtgggccgc
      361 ttcctgatga gcctggttaa ccaagtaccg aaaatagttc ccgatgactt tgagaccatg
      421 ctcaacagca acatcaatgt gagtgccctt cctgagcccc ttcttgcctg gtttcttccc
      481 ccacctcagc acatacactc aaatgtgaaa agagttgggt gaggtgacct ggagtaggat
      541 agagacaggg cttcacctat agccccactg aagtccaggt tgggaggaag gaagaggtgt
      601 gttatgcgtg gttggagact tccttccttc tcccatgatt ccattgtctc atttactgct
      661 gatactactg tgacttactg tctaaattcg tgatggaaag aaacactaaa attcagaggt
      721 taatgaaaat gcaattactt atttccccca tctgagttca cagaccccct atattccatc
      781 catgggtttc agctaaggca cccctagtct ataagtgtat cagtagagat aagaaagcct
      841 tctcaagcat ttcctttctg tcttctacct ttttgacttt tattcagtct ctcctagctg
      901 tcatttatcc acagctgtcc tcatacccag tgtctaccat agagctggcc aaagccagcc
      961 tttttgctcc ctttccaccc aaccccccac tcattgtgta ttctttgtct tccaggacct
     1021 tttgatggtg acctacctgg ccaacctcac acaatcgcaa atagctctaa atgaaaaact
     1081 tgtaaacctg ggaggaggag gaggaggatc cggtgcaggc gccaaggaga agagtgcttg
     1141 tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc caccggtgag
     1201 atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc cggaggcggc
     1261 ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg atttgaggat
     1321 gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     1441 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1501 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1621 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1741 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
     1801 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     1861 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1921 gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg
     1981 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg gcatggacga
     2041 gctgtacaag taatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     2101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     2161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2281 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2881 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3001 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3061 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3241 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     3301 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3361 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3421 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     3481 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3541 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3601 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3661 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3721 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3781 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3841 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3901 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3961 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4021 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4081 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4141 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4201 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     4261 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4321 gaataaaacg cacggtgttg ggtcgttgga tcctgaatgg accccaagca gtacacttgc
     4381 tggtctaggt attaacccca ggactcagaa gtgaaggaga aatgggtttt ttgtggtctt
     4441 gagtcacact gagatagtca gttgtgtgtg actctaataa acggagccta ccttttgtaa
     4501 attaatttca tcttatgtga gtttattgcc gggtggaagg gaggaaaatg ttttagatca
     4561 cacagaaact aggaagtgaa tattgtaggt agagaagttg gttatgtgtt tggcatgtta
     4621 ctgttttacc aaactgttct tttggttttc aatatggaaa ggtgtccatt ggcaaaacaa
     4681 ccttttcttg aggattcatg tgatttctac aggaaatgaa aataagaatc catggagaaa
     4741 aaatagctaa gaaaattgga tgcttaatct gaactaaaag agtagagtgt gtagtcacta
     4801 tcttaaaata cattaatagt aagatggagc tctttgccac tttggttcgg ggaggcagaa
     4861 ttaagatctg tgggttaaag attaaagagg tttccccaaa acttcagaaa attcttaggt
     4921 gctatctgta actcgaggat agagccttga gttatactga ggtctgccag tggctcaatt
     4981 taagcaaaga ctagatggca tttcccagag gccttgtagg ctagagtact tgcctgtgta
     5041 acttcagaca tccatttcct ctggtatcct ggagttccgc ctcgccactg tgtgtcttag
     5101 aaaaagggcg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     5161 gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     5221 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5281 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5341 ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     5401 gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc
     5461 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     5521 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     5581 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     5641 ttctggggaa tataaatgtc aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5701 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     5761 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     5821 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     5881 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     5941 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
     6001 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6061 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6121 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6181 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6241 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6301 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6361 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6421 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6481 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     6541 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6601 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6661 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6721 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6781 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
     6841 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
     6901 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6961 accctgataa atgcttcaat aatagcacgt gaggagggcc accatggcca agttgaccag
     7021 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7081 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7141 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7201 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7261 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7321 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7381 cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7441 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7501 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7561 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7621 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7681 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7741 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7801 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7861 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7921 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7981 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8041 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8101 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8161 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8221 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8281 ggaagagcgc ccaatacgca aaccgc
//
