LOCUS       sequence-381239-       10090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning ve...
FEATURES             Location/Qualifiers
     source          1..10090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        293..368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        563..866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        868..1145
                     /label=chicken beta-actin promoter
     intron          1146..2162
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2086..2108
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2170..2189
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2229..2249
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2274..6191
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      5911..5920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6192..6239
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6246..6326
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             6336..6389
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6390..6392,6393..7103)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6390..6392,6393..7103)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6432..6453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6693..6712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7040..7061
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7131..7148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7137..7344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7353..7493
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7353..7482
                     /label=AAV2 ITR
     rep_origin      7568..8023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7655..7674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7865..7886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8040..8059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8159..8181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8219..8237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8305..8409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8410..8478,8479..9270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8628..8647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9441..10029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9930..9949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg taatttctac tcttgtagat gtcttcgaga agactttttt ttgttttaga
      301 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      361 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt
      421 tagcgcgtgc gccaattctg cagacaartk sywmtwssmw yrsmrrckgs gagstycwcs
      481 kcggtggcgg ccgctctaga actagttatt aatagtaatc aattacgggg tcattagttc
      541 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      601 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      661 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      721 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      781 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      841 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
      901 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
      961 cagcgatggg ggcggggggg gggggggggc gcgcgccrgg sggggggggg sggggsgrgg
     1021 ggsggggsgg ggsgrggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1081 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1141 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1201 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1261 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     1321 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     1381 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     1441 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1501 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1561 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1621 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1681 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1741 gggcggggcc gcctcgggcc ggggrgggct cgggggaggg gcgcggcggc ccccggagcg
     1801 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1861 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1921 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1981 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2041 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2101 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2161 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaccggtcca
     2221 ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccacacagt
     2281 tcgagggctt taccaacctg tatcaggtga gcaagacact gcggtttgag ctgatcccac
     2341 agggcaagac cctgaagcac atccaggagc agggcttcat cgaggaggac aaggcccgca
     2401 atgatcacta caaggagctg aagcccatca tcgatcggat ctacaagacc tatgccgacc
     2461 agtgcctgca gctggtgcag ctggattggg agaacctgag cgccgccatc gactcctata
     2521 gaaaggagaa aaccgaggag acaaggaacg ccctgatcga ggagcaggcc acatatcgca
     2581 atgccatcca cgactacttc atcggccgga cagacaacct gaccgatgcc atcaataaga
     2641 gacacgccga gatctacaag ggcctgttca aggccgagct gtttaatggc aaggtgctga
     2701 agcagctggg caccgtgacc acaaccgagc acgagaacgc cctgctgcgg agcttcgaca
     2761 agtttacaac ctacttctcc ggcttttatg agaacaggaa gaacgtgttc agcgccgagg
     2821 atatcagcac agccatccca caccgcatcg tgcaggacaa cttccccaag tttaaggaga
     2881 attgtcacat cttcacacgc ctgatcaccg ccgtgcccag cctgcgggag cactttgaga
     2941 acgtgaagaa ggccatcggc atcttcgtga gcacctccat cgaggaggtg ttttccttcc
     3001 ctttttataa ccagctgctg acacagaccc agatcgacct gtataaccag ctgctgggag
     3061 gaatctctcg ggaggcaggc accgagaaga tcaagggcct gaacgaggtg ctgaatctgg
     3121 ccatccagaa gaatgatgag acagcccaca tcatcgcctc cctgccacac agattcatcc
     3181 ccctgtttaa gcagatcctg tccgatagga acaccctgtc tttcatcctg gaggagttta
     3241 agagcgacga ggaagtgatc cagtccttct gcaagtacaa gacactgctg agaaacgaga
     3301 acgtgctgga gacagccgag gccctgttta acgagctgaa cagcatcgac ctgacacaca
     3361 tcttcatcag ccacaagaag ctggagacaa tcagcagcgc cctgtgcgac cactgggata
     3421 cactgaggaa tgccctgtat gagcggagaa tctccgagct gacaggcaag atcaccaagt
     3481 ctgccaagga gaaggtgcag cgcagcctga agcacgagga tatcaacctg caggagatca
     3541 tctctgccgc aggcaaggag ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt
     3601 cccacgcaca cgccgccctg gatcagccac tgcctacaac cctgaagaag caggaggaga
     3661 aggagatcct gaagtctcag ctggacagcc tgctgggcct gtaccacctg ctggactggt
     3721 ttgccgtgga tgagtccaac gaggtggacc ccgagttctc tgcccggctg accggcatca
     3781 agctggagat ggagccttct ctgagcttct acaacaaggc cagaaattat gccaccaaga
     3841 agccctactc cgtggagaag ttcaagctga actttcagat gcctacactg gcctctggct
     3901 gggacgtgaa taaggagaag aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact
     3961 atctgggcat catgccaaag cagaagggca ggtataaggc cctgagcttc gagcccacag
     4021 agaaaaccag cgagggcttt gataagatgt actatgacta cttccctgat gccgccaaga
     4081 tgatcccaaa gtgcagcacc cagctgaagg ccgtgacagc ccactttcag acccacacaa
     4141 cccccatcct gctgtccaac aatttcatcg agcctctgga gatcacaaag gagatctacg
     4201 acctgaacaa tcctgagaag gagccaaaga agtttcagac agcctacgcc aagaaaaccg
     4261 gcgaccagaa gggctacaga gaggccctgt gcaagtggat cgacttcaca agggattttc
     4321 tgtccaagta taccaagaca acctctatcg atctgtctag cctgcggcca tcctctcagt
     4381 ataaggacct gggcgagtac tatgccgagc tgaatcccct gctgtaccac atcagcttcc
     4441 agagaatcgc cgagaaggag atcatggatg ccgtggagac aggcaagctg tacctgttcc
     4501 agatctataa caaggacttt gccaagggcc accacggcaa gcctaatctg cacacactgt
     4561 attggaccgg cctgttttct ccagagaacc tggccaagac aagcatcaag ctgaatggcc
     4621 aggccgagct gttctaccgc cctaagtcca ggatgaagag gatggcacac cggctgggag
     4681 agaagatgct gaacaagaag ctgaaggatc agaaaacccc aatccccgac accctgtacc
     4741 aggagctgta cgactatgtg aatcacagac tgtcccacga cctgtctgat gaggccaggg
     4801 ccctgctgcc caacgtgatc accaaggagg tgtctcacga gatcatcaag gataggcgct
     4861 ttaccagcga caagttcttt ttccacgtgc ctatcacact gaactatcag gccgccaatt
     4921 ccccatctaa gttcaaccag agggtgaatg cctacctgaa ggagcacccc gagacaccta
     4981 tcatcggcat cgctcggggc gagagaaacc tgatctatat cacagtgatc gactccaccg
     5041 gcaagatcct ggagcagcgg agcctgaaca ccatccagca gtttgattac cagaagaagc
     5101 tggacaacag ggagaaggag agggtggcag caaggcaggc ctggtctgtg gtgggcacaa
     5161 tcaaggatct gaagcagggc tatctgagcc aggtcatcca cgagatcgtg gacctgatga
     5221 tccactacca ggccgtggtg gtgctggaga acctgaattt cggctttaag agcaagagga
     5281 ccggcatcgc cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc gataagctga
     5341 attgcctggt gctgaaggac tatccagcag agaaagtggg aggcgtgctg aacccatacc
     5401 agctgacaga ccagttcacc tcctttgcca agatgggcac ccagtctggc ttcctgtttt
     5461 acgtgcctgc cccatataca tctaagatcg atcccctgac cggcttcgtg gaccccttcg
     5521 tgtggaaaac catcaagaat cacgagagcc gcaagcactt cctggagggc ttcgactttc
     5581 tgcactacga cgtgaaaacc ggcgacttca tcctgcactt taagatgaac agaaatctgt
     5641 ccttccagag gggcctgccc ggctttatgc ctgcatggga tatcgtgttc gagaagaacg
     5701 agacacagtt tgacgccaag ggcacccctt tcatcgccgg caagagaatc gtgccagtga
     5761 tcgagaatca cagattcacc ggcagatacc gggacctgta tcctgccaac gagctgatcg
     5821 ccctgctgga ggagaagggc atcgtgttca gggatggctc caacatcctg ccaaagctgc
     5881 tggagaatga cgattctcac gccatcgaca ccatggtggc cctgatccgc agcgtgctgc
     5941 agatgcggaa ctccaatgcc gccacaggcg aggactatat caacagcccc gtgcgcgatc
     6001 tgaatggcgt gtgcttcgac tcccggtttc agaacccaga gtggcccatg gacgccgatg
     6061 ccaatggcgc ctaccacatc gccctgaagg gccagctgct gctgaatcac ctgaaggaga
     6121 gcaaggatct gaagctgcag aacggcatct ccaatcagga ctggctggcc tacatccagg
     6181 agctgcgcaa caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     6241 gatcctaccc atacgatgtt ccagattacg cttatcccta cgacgtgcct gattatgcat
     6301 acccatatga tgtccccgac tatgccggca gtggagaggg cagaggaagt ctgctaacat
     6361 gcggtgacgt cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg
     6421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     6601 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     6781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     6841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     6901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     6961 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     7021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     7081 tcggcatgga cgagctgtac aaggaattct aactagagct cgctgatcag cctcgactgt
     7141 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7201 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7261 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7321 agagaatagc aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac
     7381 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     7441 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     7501 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     7561 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7621 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     7681 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7741 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     7801 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7861 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     7921 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     7981 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     8041 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8101 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8161 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9121 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10081 gctcacatgt
//
