LOCUS       sequence-38124-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Sibley MH, Raleigh EA
  TITLE     A versatile element for gene addition in bacterial chromosomes.
  JOURNAL   Nucleic Acids Res. 2012 Feb;40(3):e19. doi: 10.1093/nar/gkr1085. 
            Epub 2011 Nov 28.
  PUBMED    22123741
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-02"; volume: "40(3):e19. doi"; pages: " 
            10.1093/nar/gkr1085. Epub 2011 Nov 28"
COMMENT     Sequence Label: pMS33
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..202)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(246..364)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      449..535
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgaatccgt aatcatggtc atgacgtccc tcctgtgaat gtctcctcag ccctgcagga
      241 agctttacga ccagtctaaa aagcgcctga attcgcgacc ttctcgttac tgacaggaaa
      301 atgggccatt ggcaaccagg gaaagatgaa cgtgatgatg ttcacaattt gctgaattgt
      361 ggtgatgtga tgctcaccgc atttccttaa ttaacgccgg atggtagtgt ggggtctccc
      421 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
      481 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      541 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgg
//
