LOCUS       sequence-381240-        4563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO1A2_STOP
FEATURES             Location/Qualifiers
     source          1..4563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2688..2787)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2804..2823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2805..2823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2828..2844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2828..2844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2946..2965)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2957..3766
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3024..3043)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3913..4501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4402..4421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcg agacagagaa gcggatcgag acccaccgga tcagatgcct
      721 gtccaagctg aagatgtttc tgctggccat cacatgtgcc ttcgtgtcta agaccctgag
      781 cggctcctac atgaacagca tgctgacaca gatcgagagg cagtttaata tccccaccag
      841 cctggtgggc tttatcaacg gctccttcga gatcggcaat ctgctgctga tcatcttcgt
      901 gagctatttc ggcacaaagc tgcaccgccc catcatgatc ggaatcggat gcgtggtcat
      961 gggactgggc tgttttctga agagcctgcc tcacttcctg atgaaccagt acgagtatga
     1021 gtctaccgtg tctgtgagcg gcaacctgag ctccaatagc ttcctgtgca tggagaatgg
     1081 cacacagatc ctgcggccca cccaggatcc ttctgagtgt acaaaggagg tgaagagcct
     1141 gatgtgggtt tatgtactcg ttggcaacat cgtgagaggc atgggcgaga cccctatcct
     1201 gccactgggc atcagctaca tcgaggactt tgccaagttc gagaattccc ctctgtatat
     1261 cggactggtg gagaccggag caatcatcgg accactgatc ggactgctgc tggcaagctt
     1321 ttgcgccaac gtgtacgtgg atacaggctt cgtgaatacc gacgatctga tcatcacccc
     1381 aacagacacc agatgggtgg gcgcctggtg gtttggcttc ctgatctgtg ccggcgtgaa
     1441 cgtgctgaca gccatcccct tctttttcct gccaaacaca ctgcccaagg agggcctgga
     1501 gaccaatgcc gatatcatca agaacgagaa tgaggacaag cagaaggagg aggtgaagaa
     1561 ggagaagtac ggcatcacca aggattttct gcccttcatg aagtccctgt cttgcaaccc
     1621 tatctatatg ctgtttatcc tggtgtccgt gatccagttt aacgccttcg tgaatatgat
     1681 ctctttcatg cccaagtacc tggagcagca gtatggcatc tctagctccg acgccatctt
     1741 tctgatgggc atctacaatc tgccccctat ctgcatcggc tatatcatcg gcggcctgat
     1801 catgaagaag ttcaagatca ccgtgaagca ggcagcacac atcggatgct ggctgtccct
     1861 gctggagtac ctgctgtatt ttctgtcttt cctgatgaca tgtgagaact ctagcgtggt
     1921 gggcatcaat acctcctacg agggcatccc tcaggatctg tatgtggaga acgacatctt
     1981 tgccgattgc aacgtggact gcaattgtcc tagcaagatc tgggaccccg tgtgcggcaa
     2041 caatggactg agctacctgt ccgcctgcct ggcaggatgt gagacatcca tcggcaccgg
     2101 catcaacatg gtgttccaga attgctcctg tatccagacc tctggcaatt cctctgccgt
     2161 gctgggcctg tgcgataagg gccctgactg ttccctgatg ctgcagtatt ttctgatcct
     2221 gtccgccatg agctccttca tctactctct ggccgccatc ccaggctata tggtgctgct
     2281 gaggtgcatg aagtctgagg agaagagcct gggcgtgggc ctgcacacat tttgtacccg
     2341 cgtgttcgca ggaatcccag caccaatcta cttcggcgcc ctgatggata gcacatgcct
     2401 gcactggggc accctgaagt gcggagagag cggagcctgt aggatctatg actccaccac
     2461 atttcgctac atctatctgg gactgccagc cgccctgagg ggatctagct tcgtgcccgc
     2521 cctgatcatc ctgatcctgc tgagaaagtg ccacctgcca ggagagaacg catcctctgg
     2581 aacagagctg atcgagacca aggtgaaggg caaggagaat gagtgtaagg atatctacca
     2641 gaagagcaca gtgctgaagg acgatgagct gaagaccaag ctgtaagacc cagctttctt
     2701 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2761 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2821 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2881 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2941 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3001 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3061 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3121 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3181 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3241 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3301 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3361 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3421 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3481 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3541 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3601 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3661 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3721 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3781 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3841 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4561 gtt
//
