LOCUS       sequence-38125-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Sibley MH, Raleigh EA
  TITLE     A versatile element for gene addition in bacterial chromosomes.
  JOURNAL   Nucleic Acids Res. 2012 Feb;40(3):e19. doi: 10.1093/nar/gkr1085. 
            Epub 2011 Nov 28.
  PUBMED    22123741
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-02"; volume: "40(3):e19. doi"; pages: " 
            10.1093/nar/gkr1085. Epub 2011 Nov 28"
COMMENT     Sequence Label: pMS34
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..201)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     150..172
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     164..181
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     165..181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(258..376)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      461..547
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cggtgccgga aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgaatccgta atcatggtca tgacgtccct cctgtgtgaa attgttatga atgtctcctc
      241 agccctgcag gaagctttac gaccagtcta aaaagcgcct gaattcgcga ccttctcgtt
      301 actgacagga aaatgggcca ttggcaacca gggaaagatg aacgtgatga tgttcacaat
      361 ttgctgaatt gtggtgatgt gatgctcacc gcatttcctt aattaacgcc ggatggtagt
      421 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      481 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      541 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
      601 aggacgcccg ccataaactg
//
