LOCUS       sequence-381281-       12808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, 
            Huttenlocher A
  TITLE     Calpain-mediated proteolysis of talin regulates adhesion dynamics.
  JOURNAL   Nat Cell Biol. 2004 Oct . 6(10):977-83.
  PUBMED    15448700
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2004 Oct . 6(10):977-83."
COMMENT     Sequence Label: GFP-Talin1
FEATURES             Location/Qualifiers
     source          1..12808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8104..8225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8141..8160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8195..8214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8232..8687)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8369..8390)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8581..8600
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8714..8818
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8820..9177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8820..9011
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8843..8863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9028..9163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9212..10006
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9212..10006
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9266..9285)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9876..9895
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10194..10213)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10238..10285
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10614..11202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11103..11122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        11377..11680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11681..11884
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11835..11855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      11923..11932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11929..11931,11932..11934,11935..12645)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11929..11931,11932..11934,11935..12645)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11974..11995)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12235..12254)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12582..12603
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      12694..12703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagaggccct ggctggccct cccaacgact ttgggctctt tctgtcagat gatgacccca
       61 aaaaaggcat ctggctagag gctgggaaag ctttggacta ctacatgctc cgaaatgggg
      121 acaccatgga gtacagaaaa aagcagagac ccctgaagat ccggatgtta gacggaacgg
      181 tgaagactat catggtggat gactccaaga ccgtcacaga catgctcatg accatttgtg
      241 cccgaattgg tatcaccaac catgatgagt attcactggt tcgagagctg atggaagaaa
      301 agaaagatga ggggacaggg accctgagaa aagacaagac cctactgcga gatgaaaaga
      361 agatggagaa gctaaagcag aagttgcaca cagacgatga gttgaactgg ctggaccatg
      421 ggcggacact gagggaacag ggagtggaag agcatgagac gttgctgctg cggagaaagt
      481 tcttctactc agaccagaat gtggattccc gagaccctgt acagctgaac cttctctatg
      541 tgcaggcacg agatgacatc ctgaatggct cccatcctgt ctcctttgac aaggcctgtg
      601 aatttgcagg cttccagtgc cagatccagt ttggacctca caatgaacag aagcacaagg
      661 ctggcttcct tgacctgaag gacttcctgc ccaaggagta tgtgaagcag aagggagagc
      721 gtaagatctt tcaggcacac aagaattgtg ggcagatgag tgaaattgag gccaaggtac
      781 gctacgtgaa gctggcccgt tccctcaaga cttacggtgt ctccttcttc ctagtcaagg
      841 aaaagatgaa ggggaagaat aaactggtgc ccaggctgtt gggcatcact aaggagtgtg
      901 tgatgcgtgt ggatgagaag accaaggagg tgattcagga atggagcctc accaacatca
      961 aacgctgggc tgcctctccc aagagcttta ctctggactt tggagactat caggatggct
     1021 actactcagt acagacgaca gaaggcgagc agatcgcaca gctcattgct ggctacatag
     1081 atatcatcct taagaagaaa aaaagcaagg accattttgg gctggaggga gatgaagagt
     1141 ctactatgtt ggaggactca gtgtctccca aaaagtcaac agtccttcag cagcagtaca
     1201 accgagtggg gaaagtggag cacggctctg tggctctgcc agccatcatg cgctctggag
     1261 cctctggtcc tgagaatttc caggtgggga gcatgccacc tgcccagcag cagatcacca
     1321 gtggccagat gcaccgagga cacatgccac ctctgacttc agcccagcag gcgctcactg
     1381 gaaccattaa ctccagcatg caggctgtgc aggctgccca ggccactctg gatgactttg
     1441 agactctacc ccctcttggc caggatgctg cctccaaagc ttggcgtaag aacaagatgg
     1501 atgaatcaaa gcatgagatc cactcccagg tagatgccat cacagctggc actgcctctg
     1561 tggtaaacct gacagcagga gatcctgcag agacagacta tacagcagtg ggctgtgcag
     1621 tcaccaccat ctcttccaac ctgacggaga tgtcccgtgg ggtgaagcta ttggctgccc
     1681 tgctggagga tgaaggcggc aatggccggc cccttctgca agcagcaaag ggccttgcag
     1741 gggctgtgtc ggaactgctt cgcagtgctc agcccgccag cgctgagcct cgtcagaacc
     1801 tgctgcaggc agccgggaac gtgggccagg ccagtgggga gctgttgcag caaattgggg
     1861 aaagtgacac tgacccccac ttccaggatg ttctaatgca gctagcaaac gcagtggcaa
     1921 gtgctgcagc cgccctggtc ctcaaggcca agagtgtggc ccagcgcaca gaggattccg
     1981 ggcttcagac ccaagtgatt gctgcagcta cgcaatgtgc tctgtccact tcccagctgg
     2041 tggcctgtac caaggtggta gcacctacaa tcagctcacc tgtgtgccaa gaacagctag
     2101 tcgaggctgg gcgactggtg gccaaagctg tggagggctg tgtgtctgcc tcgcaagcag
     2161 ctacagaaga tggacagctg cttcgagggg taggagcagc agccacggct gtcacccagg
     2221 ccctcaatga gctgctgcag cacgtgaagg cccacgccac cggagctggg cctgccggtc
     2281 ggtatgacca agccactgat accatcctca ccgtcactga aaacatcttc agctccatgg
     2341 gtgatgctgg ggagatggtg cgccaggccc gcatcctggc ccaagccacc tcagacctgg
     2401 tcaatgctat caaggccgat gctgaggggg agagtgatct ggagaactct agaaagctcc
     2461 tgagtgctgc caagatcctc gctgatgcca ccgccaagat ggtggaggcg gccaagggag
     2521 cagccgccca ccctgacagt gaggaacagc agcagcgact gcgtgaagca gctgaggggc
     2581 ttcgcatggc caccaatgca gctgcgcaga acgccatcaa gaagaagttg gtgcagcgcc
     2641 tggagcatgc agccaagcaa gctgcagcct ctgcaacaca gaccattgct gcagcccaac
     2701 atgcagcctc tgcccccaag gcctctgcgg gcccccagcc cctactggtg cagagctgta
     2761 aggccgtggc agagcagatt ccgttgctgg tgcagggtgt ccgaggaagt caagctcagc
     2821 ctgacagccc tagtgctcag ctcgccctca ttgctgccag ccagagcttc ctgcagccag
     2881 gtgggaagat ggtggcagcg gcaaaagcct cagtgccaac aattcaggac caggcttcag
     2941 cgatgcagct gagtcagtgc gcaaagaacc taggcactgc cctggccgag ctgcgcactg
     3001 ctgctcagaa ggctcaggag gcatgtggac ctttggagat ggattctgca ctgagtgttg
     3061 tacaaaatct agagaaagac ctacaggaaa taaaagcagc agctcgagat ggcaagctta
     3121 agcccttacc tggggaaaca atggagaagt gtacccaaga tcttggcaac agcaccaaag
     3181 cggtgagctc tgccatcgcc aagctgctgg gggagattgc ccagggcaac gagaactatg
     3241 caggtattgc agctcgggat gtagctggtg gactaaggtc actagcccag gctgcgcggg
     3301 gtgtggctgc gctgacatca gatcctgcag tgcaggccat tgtgcttgac acagccagcg
     3361 acgttctgga caaggccagc agcctcattg aagaggcaaa aaaagcatct ggacacccag
     3421 gggacccaga aagccagcaa aggcttgctc aggtagctaa agcagtgacc caagccctga
     3481 accgctgtgt cagctgcctg cctggccaaa gagacgtgga taatgcccta cgagccgttg
     3541 gagatgccag caagcgactc ctgagtgact tgcttcctcc aagcacgggg acatttcaag
     3601 aagcacagag ccgattgaat gaagctgcag ctgggttaaa tcaggccgcc acagaactgg
     3661 tgcaagcctc ccgaggaact cctcaggacc tggctcgggc ctccggccga tttggacagg
     3721 acttcagcac cttcctggaa gctggtgtgg agatggccgg acaggccccg agccaggagg
     3781 accgagccca ggtggtgtcc aacctgaagg gcatatccat gtcttcaagc aaacttctcc
     3841 ttgccgctaa ggccttgtcc acagaccctg cttctcccaa cctcaagagt cagctggctg
     3901 cggctgcccg ggcagtgacg gacagcatca accagctcat caccatgtgc acccagcagg
     3961 cacctggcca gaaggagtgt gacaatgcac ttcggcagct agagacagtc cgagaactcc
     4021 tggagaatcc agtccagccc atcaacgaca tgtcctactt cggttgcttg gacagtgtca
     4081 tggagaactc caaggtccta ggtgaggcca tgactggcat ctcccaaaat gccaagaatg
     4141 gaaatctgcc ggagtttgga gatgccattg ccacagcctc caaggctctc tgtggcttca
     4201 cggaggcagc cgcacaggca gcatatctag ttggtgtctc tgaccccaac agccaagctg
     4261 gacagcaagg actggtggaa cccacacagt ttgcccgtgc aaaccaggca attcagatgg
     4321 cctgtcagag tctgggggag cctggctgta cccaggccca ggtgttatct gcagccacta
     4381 ttgtagccaa acacacatct gcattgtgta acagctgtcg cctggcttcc gctaggactg
     4441 ccaatcccac tgccaagcgc cagtttgtac agtcagccaa ggaggtggcc aacagtacag
     4501 ccaatcttgt caagaccatc aaggcactag atggggactt cacagaagag aaccgtgccc
     4561 agtgccgagc agccacagcc cctctgctgg aagctgtgga taacctgagt gcctttgcct
     4621 ccaaccctga gttctccagc gtccctgccc agatcagccc tgagggcaga gcagccatgg
     4681 agcccattgt aatctctgct aagacaatgt tggagagtgc tggagggctc atccagacag
     4741 ctcgggcgtt agcagtcaat ccccgagacc ccccacgttg gtctgtgcta gctggccact
     4801 ctcggactgt ctcagattcc atcaagaaac ttattacaag catgagggac aaagccccag
     4861 ggcagctgga gtgtgagaca gccattgcgg ctctgaacag ctgcttgcgg gacctagatc
     4921 aggcttcgct tgccgctgtc agccagcagc ttgctccccg tgaaggaatc tctcaagagg
     4981 ctttgcacac ccagatgctc actgcagtgc aagaaatttc tcatctcatt gagccgctgg
     5041 ccagcgctgc tcgggctgaa gcctcccagc tgggacacaa ggtgtcccaa atggcccagt
     5101 actttgaacc actcaccctg gctgcagtgg gtgctgcgtc taagaccctg agccacccgc
     5161 agcagatggc acttctggac cagactaaaa cgttggcaga gtctgccttg cagttgctat
     5221 atactgccaa ggaggctggt ggtaacccca agcaagcagc acacacccag gaagccctgg
     5281 aggaggctgt gcagatgatg acagaggccg tagaagacct gacgacgacc ctcaacgaag
     5341 cagccagtgc tgcaggagtc gttggcggca tggtggactc tatcactcag gccatcaacc
     5401 agctagatga aggacctatg ggtgacccag aaggctcatt cgtagattac cagacaacca
     5461 tggtgaggac agccaaggcc attgctgtca ctgttcagga gatggtaacc aagtcaaaca
     5521 ccagccctga agagctaggc cctcttgcca accagctgac cagtgactat ggccgactgg
     5581 cctcacaagc caagcctgca gctgtggctg ctgaaaatga agagataggc gctcacatca
     5641 aacaccgagt acaggagctg ggccatggct gctctgctct ggtcaccaag gcaggtgcct
     5701 tgcagtgtag ccccagtgat gtctacacca agaaggagct catagagtgt gcccgcagag
     5761 tgtcagagaa ggtctcccat gttctggctg cactccaggc tgggaatcgt ggtacccagg
     5821 cctgcattac agcagccagt gctgtgtctg gtatcattgc tgacctcgac accaccatca
     5881 tgttcgctac tgctggcaca cttaaccgtg agggtgctga aacttttgct gaccaccggg
     5941 agggtatctt aaagacagca aaggttcttg tggaggacac caaggtccta gtgcagaatg
     6001 cagctgggag ccaggagaag ttggcacaag ccgcccagtc ctccgtggcc accattaccc
     6061 gcctcgctga tgtggtcaag ctcggtgcag ccagcctagg agccgaagac cctgaaactc
     6121 aggtggtgct gatcaatgca gtaaaggacg tagccaaggc cctgggtgac ctcatcagcg
     6181 ctacgaaggc tgcagcgggc aaagttgggg atgaccctgc agtgtggcag ctcaagaact
     6241 ctgccaaggt gatggtgacc aatgtgacat cattgctcaa gacagtgaag gctgtggaag
     6301 atgaggccac caaaggcaca cgggccctag aggcaaccac agagcacata cgtcaggaac
     6361 tggcggtctt ctgttcccca gagccacctg ccaagacctc tacccctgaa gacttcatcc
     6421 gaatgaccaa gggtattact atggcaacag ccaaagccgt tgctgctggc aattcctgtc
     6481 gacaggaaga tgtcattgcc acagccaatc tgagccgacg ggctattgcg gacatgcttc
     6541 gggcttgcaa ggaagcagct ttccacccag aagtggcgcc tgatgtacgg ctccgagccc
     6601 tgcactatgg ccgggagtgt gccaatggtt acctggaact gctggaccac gtgctgctga
     6661 cccttcagaa gccaaaccca gacctgaagc agcagctaac tggacactca aagcgggttg
     6721 ctggctctgt gactgagctc atccaggctg ctgaagccat gaaaggaaca gagtgggtgg
     6781 acccagagga ccctactgtc attgctgaga atgagctctt gggagccgca gccgccatcg
     6841 aggctgcagc caagaagctg gagcagctga agcccagggc caaacccaag gaggcggatg
     6901 agtccttgaa ctttgaggaa caaatcctag aagctgccaa gtccatcgct gcagccacca
     6961 gtgcactggt aaaggctgcg tcagcagccc agagggagct ggtggctcaa ggaaaggtgg
     7021 gcgccattcc agccaatgca ctggatgatg ggcagtggtc gcagggcctc atttccgctg
     7081 cccgtatggt ggctgcggcc accaacaatc tgtgtgaagc agctaacgca gctgtccagg
     7141 gccatgctag ccaggagaaa ctcatttcct cagccaagca agtagctgcc tccacagctc
     7201 agctcctggt agcttgcaag gtcaaagcag atcaggactc tgaggcaatg aaacggctcc
     7261 aggctgctgg caatgcagtg aagagggcct cagataacct ggtaaaggcg gcccagaaag
     7321 ctgcagcctt cgaagaccag gagaatgaga cggtggtggt gaaggagaaa atggttgggg
     7381 gcattgccca gattatcgca gcacaggaag agatgcttcg gaaggaacga gagctggaag
     7441 aggctcggaa aaagctcgcc cagatccggc agcagcagta caagttcttg ccttcagagc
     7501 ttcgagacga gcactaaaaa agccctgtgt atttaatgca gacccagccc agagactgtg
     7561 cctgccacta ccaaagcctt ctgggctgct caggacccaa gctgcccaac cccagcactc
     7621 ccccaaagtg cctgccaaac cctgggcctg gccctgccca gtcccactgc aagccgtgtc
     7681 ctctccctga ctcccaagtg cctttgcacc ctagggcccc taagtgcctg cccccctcca
     7741 gagtattaac gctccaagag tattattaat gctgccgtac ctcagtttga acctgccagg
     7801 gccccagctg ctccagcctg ccagcagctt ccagccagtc cccacggcca cgtcagctca
     7861 actcatccct ttttgatact atatccccta cccagctacc tatggggctt gagggttgta
     7921 aacccaaaca ggtcagactc ccggaattct gcagtcgacg gtaccgcggg cccgggatcc
     7981 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     8041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     8101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8221 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     8281 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     8341 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     8401 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     8461 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     8521 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     8581 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     8641 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     8701 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8761 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     8821 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     9181 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     9241 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9301 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9361 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     9421 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     9481 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     9541 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     9601 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     9661 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     9721 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     9781 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     9841 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9901 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     9961 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10021 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    10081 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10141 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
    10201 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
    10261 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
    10321 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
    10381 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
    10441 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
    10501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10741 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11221 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    11281 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
    11341 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    11401 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    11461 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    11521 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    11581 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    11641 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    11701 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    11761 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
    11821 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    11881 agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag
    11941 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    12001 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    12061 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    12121 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
    12181 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    12241 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    12301 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    12361 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    12421 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    12481 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    12541 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    12601 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
    12661 cgagctcaag ctagcttcga attcgcagaa gctgccacca tggttgcgct ttcgctgaag
    12721 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
    12781 gcctgccgca tgattcgtga gcggatcc
//
