LOCUS       sequence-381292-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1-tdTomato targeting vector, compartible with Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: AAVS1-tdTomato targeting vector (compatible wi...
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(61..67,68..85,86..91))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(244..261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(415..1003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(495..514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1174..1965,1966..2034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1797..1816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2035..2139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2207..2225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2845..3346
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             3585..4184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3585..3604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4081..4101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4216..4233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4222..4446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4452..4831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4833..5110
                     /label=chicken beta-actin promoter
     intron          5111..6127
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6051..6073
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6135..6154
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6189..7619
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(7729..7748)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7794..7849
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7848..7867)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8210..8226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8234..8250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8258..8264,8265..8282,8283..8288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8303..8324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8398..9234
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gcggccgcca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2221 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattccctgc
     2641 aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc tgggcctgtg
     2701 ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc
     2761 tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt
     2821 tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc
     2881 ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa
     2941 ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat
     3001 ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt
     3061 cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg
     3121 ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat
     3181 tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt
     3241 ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta
     3301 gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc
     3361 agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct
     3421 ctggttctgg gtacgtatgg caagcttctg acctcttctc ttcctcccac wknngrcctc
     3481 kwswswtcyk scmrcagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc
     3541 taacatgcgg tgacgtggag gagaatccca gccctaggct cgagatgacc gagtacaagc
     3601 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     3661 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3721 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3781 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3841 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3901 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3961 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4021 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4081 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4141 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga gggcccgttt
     4201 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4261 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4321 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4381 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4441 ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     4501 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     4561 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     4621 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     4681 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4741 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4801 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     4861 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     4921 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg
     4981 sggggggsgg ggsggggsga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     5041 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     5101 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5161 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5221 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     5281 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     5341 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     5401 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     5461 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     5521 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     5581 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     5641 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     5701 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     5761 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     5821 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     5881 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     5941 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     6001 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     6061 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     6121 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaccg
     6181 gtccaaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     6241 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6301 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     6361 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     6421 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6481 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6541 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6601 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6661 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6721 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6781 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6841 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     6901 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     6961 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     7021 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     7081 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     7141 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     7201 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     7261 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     7321 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     7381 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     7441 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     7501 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7561 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaat
     7621 taattaagat atcgtcgaca cgcgtgaatt cactcctcag gtgcaggctg cctatcagaa
     7681 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     7741 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     7801 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     7861 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     7921 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     7981 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     8041 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     8101 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     8161 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     8221 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8281 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8341 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cgacagtact aagctttact
     8401 agggacagga ttggtgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg
     8461 atattgggtc taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca
     8521 tttcctggag ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag
     8581 agaggatcct gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc
     8641 tgcccggttc tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg
     8701 cggctgtctg gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg
     8761 agaagcagtt tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct
     8821 tcacctttct agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata
     8881 aggccagtag ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga
     8941 aaactccctt tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact
     9001 ccctttctct ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat
     9061 tcctccgccc agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga
     9121 gtccttggca agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat
     9181 ctcatgcccc tggctctcct gccccttccc tacaggggtt cctggctctg ctctaagggc
     9241 gaattc
//
