LOCUS       sequence-381295-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 
            promoter driven guide RNA that target tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CMV:AsCpf1-2A-GFP-U6-tdTomato-sg
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        300..375
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        472..757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        759..1036
                     /label=chicken beta-actin promoter
     intron          1037..1264
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1280..1289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1292..1312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1337..5254
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4974..4983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5255..5302
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5318..5371
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5372..5374,5375..6085)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5372..5374,5375..6085)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5414..5435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5675..5694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6022..6043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6113..6130)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6119..6326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6335..6475
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6335..6464
                     /label=AAV2 ITR
     rep_origin      6550..7005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6637..6656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6847..6868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7022..7041)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7141..7163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7201..7219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7287..7391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7392..7460,7461..8252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7610..7629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8423..9011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8912..8931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg taatttctac tcttgtagat atgacctcct cgcccttgct cacttttttg
      301 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      361 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
      421 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac ccgttacata
      481 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      541 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      601 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      661 cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg
      721 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      781 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      841 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccrg gsggggsggg
      901 gsggggsgrg gggsggggsg gggsgrggcg grgaggtgcg gcggcagcca atcagagcgg
      961 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1021 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1081 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1141 ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg
     1201 gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt
     1261 tcaggttgga ccggtggccg ccaccatggc cccaaagaag aagcggaagg tcggtatcca
     1321 cggagtccca gcagccacac agttcgaggg ctttaccaac ctgtatcagg tgagcaagac
     1381 actgcggttt gagctgatcc cacagggcaa gaccctgaag cacatccagg agcagggctt
     1441 catcgaggag gacaaggccc gcaatgatca ctacaaggag ctgaagccca tcatcgatcg
     1501 gatctacaag acctatgccg accagtgcct gcagctggtg cagctggatt gggagaacct
     1561 gagcgccgcc atcgactcct atagaaagga gaaaaccgag gagacaagga acgccctgat
     1621 cgaggagcag gccacatatc gcaatgccat ccacgactac ttcatcggcc ggacagacaa
     1681 cctgaccgat gccatcaata agagacacgc cgagatctac aagggcctgt tcaaggccga
     1741 gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg agcacgagaa
     1801 cgccctgctg cggagcttcg acaagtttac aacctacttc tccggctttt atgagaacag
     1861 gaagaacgtg ttcagcgccg aggatatcag cacagccatc ccacaccgca tcgtgcagga
     1921 caacttcccc aagtttaagg agaattgtca catcttcaca cgcctgatca ccgccgtgcc
     1981 cagcctgcgg gagcactttg agaacgtgaa gaaggccatc ggcatcttcg tgagcacctc
     2041 catcgaggag gtgttttcct tcccttttta taaccagctg ctgacacaga cccagatcga
     2101 cctgtataac cagctgctgg gaggaatctc tcgggaggca ggcaccgaga agatcaaggg
     2161 cctgaacgag gtgctgaatc tggccatcca gaagaatgat gagacagccc acatcatcgc
     2221 ctccctgcca cacagattca tccccctgtt taagcagatc ctgtccgata ggaacaccct
     2281 gtctttcatc ctggaggagt ttaagagcga cgaggaagtg atccagtcct tctgcaagta
     2341 caagacactg ctgagaaacg agaacgtgct ggagacagcc gaggccctgt ttaacgagct
     2401 gaacagcatc gacctgacac acatcttcat cagccacaag aagctggaga caatcagcag
     2461 cgccctgtgc gaccactggg atacactgag gaatgccctg tatgagcgga gaatctccga
     2521 gctgacaggc aagatcacca agtctgccaa ggagaaggtg cagcgcagcc tgaagcacga
     2581 ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg aggccttcaa
     2641 gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc ctggatcagc cactgcctac
     2701 aaccctgaag aagcaggagg agaaggagat cctgaagtct cagctggaca gcctgctggg
     2761 cctgtaccac ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg accccgagtt
     2821 ctctgcccgg ctgaccggca tcaagctgga gatggagcct tctctgagct tctacaacaa
     2881 ggccagaaat tatgccacca agaagcccta ctccgtggag aagttcaagc tgaactttca
     2941 gatgcctaca ctggcctctg gctgggacgt gaataaggag aagaacaatg gcgccatcct
     3001 gtttgtgaag aacggcctgt actatctggg catcatgcca aagcagaagg gcaggtataa
     3061 ggccctgagc ttcgagccca cagagaaaac cagcgagggc tttgataaga tgtactatga
     3121 ctacttccct gatgccgcca agatgatccc aaagtgcagc acccagctga aggccgtgac
     3181 agcccacttt cagacccaca caacccccat cctgctgtcc aacaatttca tcgagcctct
     3241 ggagatcaca aaggagatct acgacctgaa caatcctgag aaggagccaa agaagtttca
     3301 gacagcctac gccaagaaaa ccggcgacca gaagggctac agagaggccc tgtgcaagtg
     3361 gatcgacttc acaagggatt ttctgtccaa gtataccaag acaacctcta tcgatctgtc
     3421 tagcctgcgg ccatcctctc agtataagga cctgggcgag tactatgccg agctgaatcc
     3481 cctgctgtac cacatcagct tccagagaat cgccgagaag gagatcatgg atgccgtgga
     3541 gacaggcaag ctgtacctgt tccagatcta taacaaggac tttgccaagg gccaccacgg
     3601 caagcctaat ctgcacacac tgtattggac cggcctgttt tctccagaga acctggccaa
     3661 gacaagcatc aagctgaatg gccaggccga gctgttctac cgccctaagt ccaggatgaa
     3721 gaggatggca caccggctgg gagagaagat gctgaacaag aagctgaagg atcagaaaac
     3781 cccaatcccc gacaccctgt accaggagct gtacgactat gtgaatcaca gactgtccca
     3841 cgacctgtct gatgaggcca gggccctgct gcccaacgtg atcaccaagg aggtgtctca
     3901 cgagatcatc aaggataggc gctttaccag cgacaagttc tttttccacg tgcctatcac
     3961 actgaactat caggccgcca attccccatc taagttcaac cagagggtga atgcctacct
     4021 gaaggagcac cccgagacac ctatcatcgg catcgatcgg ggcgagagaa acctgatcta
     4081 tatcacagtg atcgactcca ccggcaagat cctggagcag cggagcctga acaccatcca
     4141 gcagtttgat taccagaaga agctggacaa cagggagaag gagagggtgg cagcaaggca
     4201 ggcctggtct gtggtgggca caatcaagga tctgaagcag ggctatctga gccaggtcat
     4261 ccacgagatc gtggacctga tgatccacta ccaggccgtg gtggtgctgg agaacctgaa
     4321 tttcggcttt aagagcaaga ggaccggcat cgccgagaag gccgtgtacc agcagttcga
     4381 gaagatgctg atcgataagc tgaattgcct ggtgctgaag gactatccag cagagaaagt
     4441 gggaggcgtg ctgaacccat accagctgac agaccagttc acctcctttg ccaagatggg
     4501 cacccagtct ggcttcctgt tttacgtgcc tgccccatat acatctaaga tcgatcccct
     4561 gaccggcttc gtggacccct tcgtgtggaa aaccatcaag aatcacgaga gccgcaagca
     4621 cttcctggag ggcttcgact ttctgcacta cgacgtgaaa accggcgact tcatcctgca
     4681 ctttaagatg aacagaaatc tgtccttcca gaggggcctg cccggcttta tgcctgcatg
     4741 ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc aagggcaccc ctttcatcgc
     4801 cggcaagaga atcgtgccag tgatcgagaa tcacagattc accggcagat accgggacct
     4861 gtatcctgcc aacgagctga tcgccctgct ggaggagaag ggcatcgtgt tcagggatgg
     4921 ctccaacatc ctgccaaagc tgctggagaa tgacgattct cacgccatcg acaccatggt
     4981 ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat gccgccacag gcgaggacta
     5041 tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt ttcagaaccc
     5101 agagtggccc atggacgccg atgccaatgg cgcctaccac atcgccctga agggccagct
     5161 gctgctgaat cacctgaagg agagcaagga tctgaagctg cagaacggca tctccaatca
     5221 ggactggctg gcctacatcc aggagctgcg caacaaaagg ccggcggcca cgaaaaaggc
     5281 cggccaggca aaaaagaaaa aggaattcgg cagtggagag ggcagaggaa gtctgctaac
     5341 atgcggtgac gtcgaggaga atcctggccc agtgagcaag ggcgaggagc tgttcaccgg
     5401 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5461 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5521 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5581 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5641 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5701 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5761 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5821 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5881 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5941 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6001 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6061 tctcggcatg gacgagctgt acaaggaatt ctaactagag ctcgctgatc agcctcgact
     6121 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6181 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6241 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6301 gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat ggagttggcc
     6361 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     6421 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     6481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     6541 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6601 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     6661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     6781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6841 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     6901 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     6961 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     7021 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7081 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7141 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8101 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9061 ttgctcacat gt
//
