LOCUS       sequence-381296-        7839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mClover-NeoR to C-terminus of 
            endogenous human eIF3e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3e- pMK289 (mAID-mClover-NeoR) plasmid
FEATURES             Location/Qualifiers
     source          1..7839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    94..115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(130..135,136..153,154..160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     173..195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     192..208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        226..244
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     226..243
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     646..665
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(853..1647)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(853..1647)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1996..2298)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2098..2117)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     2421..2443
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2435..2452
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2436..2452
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2459..2478
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2459..2477
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3390..3593
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(3600..3602,3603..3605,3606..4319)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3645..3666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4253..4274
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4437..4558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4474..4493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4528..4547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4565..5020)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4702..4723)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4914..4933
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5047..5151
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5153..5510
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5153..5344
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5176..5196)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5361..5496
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5545..6339
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5545..6339
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      6539..6548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6545..6919
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7057..7645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7546..7565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7799..7816
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta
      241 tagaatactc aagctatgca tcaagcttgg taccatagta agacaaagtg aaaaaattga
      301 gggaaacagg gttcagccta cagggacttc tctgtactat ttttgcaaat tcctctgaat
      361 ataattattc caaaaaagaa agttaaaaaa acacaaactt gcacatgcaa gaaaactgac
      421 agcaagataa aatgaatcaa ttttattcca attcttcaaa atttatacgt aatatgttgt
      481 ttccaaaatg taagtcaccc tttatataat agttttatta tttcatcttt ctttgatagt
      541 tttttttttc atcttttctt tatggttctt caggatccaa cgacccaaca ccgtgcgttt
      601 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
      661 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
      721 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
      781 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
      841 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
      901 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
      961 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     1021 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     1081 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     1141 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     1201 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     1261 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     1321 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     1381 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     1441 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
     1501 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     1561 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     1621 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
     1681 gcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     1741 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     1801 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     1861 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     1921 ccgtttctgc ggactggctt tctacgtgaa aaggatctag gtgaagatcc tttttgataa
     1981 tctcatgcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     2041 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
     2101 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     2161 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgccccgg
     2221 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     2281 ggtgtaaacc ttaaactgcc gtacgtatag gctgcgcaac tgttgggaag ggcgatcggt
     2341 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2401 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     2461 atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg
     2521 tgatggatat ctgcagaatt cgcccttcat cattttgata ctggcagatg ataacttaca
     2581 tgtgtagccc tctttcaagt gttcgaaatg tgtattcttt ctaaatctaa agaaataatt
     2641 ctgtaaaaat gttgatctta ttatatgtat tagtgctgat cttatacaaa tttcattatc
     2701 tttcatctga tattcatatc aaaaattaat ctcccatttt gatagagatc ctgcatgtta
     2761 gagattgaag aaacttgaat ggccttataa ttttggaatc ctaaactgtg ttgtttagac
     2821 attatgatta tgtgtaactg gagggtagga agtcattagg aagcagttgt gggatatgca
     2881 gagaccccat ttgattgaaa ttttaggaac aaatttgtgt ccgcagaata atcctttagt
     2941 tcaaatgaga aggatgcatt agaattcaag ttgcatattt tagccaagtt tagaataact
     3001 gagcctagaa agtaactttc atatgatgtt tttttctgta tgctttagga ttaatttgta
     3061 tcatttattg ttccagtatt aatgatactg ttattgctat atattttaat tggccaaaag
     3121 ctataccaga taataatttt ggtgtttgtt ttgtcctgag tgggacatct gacatctagt
     3181 aattgcagga gttaagatct tagaatggtt tggcaagaga ctatagtgct gaagtaaatg
     3241 ctgtgttact ttcttctatg ttagtttttt tccgaaagta ttttcttttc acggtgttga
     3301 tttctttttg catattttag gctcctaact gggcaactca agattctggt ttctatggag
     3361 gaggaggagg aggatccggt gcaggcgcca aggagaagag tgcttgtcct aaagatccag
     3421 ccaaacctcc ggccaaggca caagttgtgg gatggccacc ggtgagatca taccggaaga
     3481 acgtgatggt ttcctgccaa aaatcaagcg gtggcccgga ggcggcggcg ttcgtgaagg
     3541 tatcaatgga cggagcaccg tacttgagga aaatcgattt gaggatgtat aaagctagca
     3601 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3661 gcgacgtaaa cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg
     3721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3781 tcgtgaccac cttcggctac ggcgtggcct gcttcagccg ctaccccgac cacatgaagc
     3841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt
     3901 tcaaggacga cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4021 agctggagta caacttcaac agccacaacg tctatatcac ggccgacaag cagaagaacg
     4081 gcatcaaggc taacttcaag atccgccaca acgttgagga cggcagcgtg cagctcgccg
     4141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4201 acctgagcca tcagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4261 tgctggagtt cgtgaccgcc gccgggatta cacatggcat ggacgagctg tacaagtaat
     4321 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4381 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     4561 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4621 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4681 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4741 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4801 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4861 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4921 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4981 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5161 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5221 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5341 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5401 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5461 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     5521 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5581 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5641 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5701 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     5761 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5821 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5881 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5941 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6001 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6061 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6121 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6181 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6241 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6301 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc
     6361 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac
     6421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc
     6541 caccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     6601 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     6661 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     6721 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     6781 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     6841 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     6901 ggccgaggag caggactgac acgtgctaaa acttcatttt taatttaaaa ggatctaggt
     6961 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7021 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7081 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7141 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7201 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7261 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7321 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7381 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7441 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7501 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7561 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7621 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7681 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7741 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7801 cgagtcagtg agcgaggaag cggaagagcg cccaatacg
//
