LOCUS       sequence-381299-        6000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses SERCaMP under control of the 
            CaMKII promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  TITLE     Harvey Lab Plasmids 2022
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOttc1494 - pAAV CaMKII SERCaMP-No Tag
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    2127..2715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2180..2200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2598..2609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2747..3223
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2946..2965)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3263..3403
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3263..3392
                     /label=AAV2 ITR
     rep_origin      3478..3933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3565..3584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3775..3796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3950..3969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4069..4091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4129..4147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4215..4319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4320..4388,4389..5180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4538..4557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5351..5939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5840..5859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cggatccgaa gttatcgcta gcgccaccat gtgggccacg caggggctgg
     1501 cggtggcgct ggctctgagc gtgctgccgg gcagccgggc gctgcggccg accgagaaca
     1561 acgaagactt caacatcgtg gccgtggcca gcaacttcgc gaccacggat ctcgatgctg
     1621 accgcgggaa gttgcccggc aagaagctgc cgctggaggt gctcaaagag atggaagcca
     1681 atgcccggaa agctggctgc accaggggct gtctgatctg cctgtcccac atcaagtgca
     1741 cgcccaagat gaagaagttc atcccaggac gctgccacac ctacgaaggc gacaaagagt
     1801 ccgcacaggg cggcataggc gaggcgatcg tcgacattcc tgagattcct gggttcaagg
     1861 acttggagcc catggagcag ttcatcgcac aggtcgatct gtgtgtggac tgcacaactg
     1921 gctgcctcaa agggcttgcc aacgtgcagt gttctgacct gctcaagaag tggctgccgc
     1981 aacgctgtgc gacctttgcc agcaagatcc agggccaggt ggacaagatc aagggcgccg
     2041 gtggtgacta gggcgcgccg aattcacgcg taccggttag taatgaggcg cgccataact
     2101 tcgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2161 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2221 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2281 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2341 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2401 ccggaacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2461 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2521 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt
     2581 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2641 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2701 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg
     2761 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
     2821 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
     2881 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
     2941 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
     3001 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
     3061 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
     3121 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
     3181 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac
     3241 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     3301 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     3361 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     3421 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     3481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3541 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3661 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     3721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3781 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     3841 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3901 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     3961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     4141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
