LOCUS       sequence-38132-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Terrell J, Shih S, Dunn R, Hicke L
  TITLE     A function for monoubiquitination in the internalization of a G 
            protein-coupled receptor.
  JOURNAL   Mol Cell. 1998 Jan;1(2):193-202.
  PUBMED    9659916
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "1998-01"; volume: "1(2)"; pages: "193-202"
COMMENT     Sequence Label: LHP306 - Ub - no Lys
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..235
                     /codon_start=1
                     /product="human ubiquitin variant in which all of the 
                     lysines have been mutated to arginine"
                     /label=ubiquitin-K0
                     /note="also known as ubiquitin-KO"
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     terminator      348..595
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(364..382)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 attcattatg cagatcttcg tcagaacgtt aaccggtaga accataactc tagaagttga
       61 accatccgat accatcgaaa acgttagagc tagaattcaa gacagagaag gcattccacc
      121 tgatcaacaa agattgatct ttgccggtag acagctcgag gacggtagaa cgctgtctga
      181 ttacaacatt cagagagagt cgaccttaca tcttgtctta agactaagag gtggttgagg
      241 taccaagatg gcctttggtg ggttgaagaa ggaaaaagac agaaacgact taattaccta
      301 cttgaaaaaa gcctgtgagt aaacaggccc cttttccttt gtcgatatca tgtaattagt
      361 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
      421 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
      481 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
      541 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct
      601 tatcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
      661 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
      721 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
      781 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
      841 aacaataata atgtc
//
