LOCUS       sequence-381346-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses BirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Alteen MG, Deme JC, Alvarez CP, Loppnau P, Hutchinson A, Seitova A, 
            Chandrasekaran R, Silva Ramos E, Secker C, Alqazzaz M, Wanker EE, 
            Lea SM, Arrowsmith CH, Harding RJ
  TITLE     Delineation of functional subdomains of Huntingtin protein and their
            interaction with HAP40.
  JOURNAL   Structure. 2023 Sep 7;31(9):1121-1131.e6. doi: 
            10.1016/j.str.2023.06.002. Epub 2023 Jun 29.
  PUBMED    37390814
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2023.06.002"; journalName: "Structure"; date: 
            "2023-09-7- 7"; volume: "31"; issue: "9"; pages: "1121-1131.e6"
COMMENT     Sequence Label: BirA_BVES untagged
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..963
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1..963
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 atgaaggata acaccgtgcc actgaaattg attgccctgt tagcgaacgg tgaatttcac
       61 tctggcgagc agttgggtga aacgctggga atgagccggg cggctattaa taaacacatt
      121 cagacactgc gtgactgggg cgttgatgtc tttaccgttc cgggtaaagg atacagcctg
      181 cctgagccta tccagttact taatgctaaa cagatattgg gtcagctgga tggcggtagt
      241 gtagccgtgc tgccagtgat tgactccacg aatcagtacc ttcttgatcg tatcggagag
      301 cttaaatcgg gcgatgcttg cattgcagaa taccagcagg ctggccgtgg tcgccggggt
      361 cggaaatggt tttcgccttt tggcgcaaac ttatatttgt cgatgttctg gcgtctggaa
      421 caaggcccgg cggcggcgat tggtttaagt ctggttatcg gtatcgtgat ggcggaagta
      481 ttacgcaagc tgggtgcaga taaagttcgt gttaaatggc ctaatgacct ctatctgcag
      541 gatcgcaagc tggcaggcat tctggtggag ctgactggca aaactggcga tgcggcgcaa
      601 atagtcattg gagccgggat caacatggca atgcgccgtg ttgaagagag tgtcgttaat
      661 caggggtgga tcacgctgca ggaagcgggg atcaatctcg atcgtaatac gttggcggcc
      721 atgctaatac gtgaattacg tgctgcgttg gaactcttcg aacaagaagg attggcacct
      781 tatctgtcgc gctgggaaaa gctggataat tttattaatc gcccagtgaa acttatcatt
      841 ggtgataaag aaatatttgg catttcacgc ggaatagaca aacagggggc tttattactt
      901 gagcaggatg gaataataaa accctggatg ggcggtgaaa tatccctgcg tagtgcagaa
      961 aaa
//
