LOCUS       sequence-381348-       10255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HIV-1 Env.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Herschhorn A, Finzi A, Jones DM, Courter JR, Sugawara A, Smith AB 
            3rd, Sodroski JG
  TITLE     An inducible cell-cell fusion system with integrated ability to 
            measure the efficiency and specificity of HIV-1 entry inhibitors.
  JOURNAL   PLoS One. 2011;6(11):e26731. doi: 10.1371/journal.pone.0026731. Epub
            2011 Nov 1.
  PUBMED    22069466
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e26731. doi"; pages: " 
            10.1371/journal.pone.0026731. Epub 2011 Nov 1"
COMMENT     Sequence Label: plvx-AD8
FEATURES             Location/Qualifiers
     source          1..10255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2393..2411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2423..2461
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2458..2482
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4463..4507
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4801..4827
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        5088..5587
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5123..5146)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5520..5539
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5608..6207
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5608..5627)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6104..6124
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6224..6812
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6277..6297)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6695..6706)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7019..7652
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7780..7796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7780..7796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7793..7815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7804..7820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7828..7834,7835..7852,7853..7858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8011..8028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8182..8770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8262..8281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8941..9732,9733..9801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9564..9583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9802..9906)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9954..10088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9991..10010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10045..10064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttantccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2401 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     2461 tcgtttagtg aaccgtcaga tcgcctggag aaggatccgc ggccgcacca tgaaggtgaa
     2521 gggcatccgc aagaactacc agcacctgtg gaagtggggc atcatgctgc tgggaatgct
     2581 gatgatctgc tccgccgtgg agaacctgtg ggtgaccgtg tactatggcg tgcccgtctg
     2641 gaaggaggcc accacaaccc tgttctgcgc cagcgatgcc aaggcctacg acaccgaggt
     2701 gcacaacgtc tgggccaccc acgcctgcgt gccaaccgat cccaatcccc aggaggtggt
     2761 gctggagaac gtgaccgaga acttcaacat gtggaagaac aacatggtgg agcagatgca
     2821 cgaagacatc atcagcctgt gggaccagag cctgaagccc tgcgtgaagc tgacacccct
     2881 gtgtgtgacc ctgaactgca cagacctgcg caacgtgacc aacatcaaca acagcagcga
     2941 gggaatgcgc ggcgagatca agaactgcag cttcaacatc accaccagca tccgcgacaa
     3001 ggtgaagaag gactacgccc tgttctaccg cctggacgtg gtgcccatcg acaacgacaa
     3061 caccagctac cgcctgatca actgcaacac cagcaccatc acccaggcat gccccaaggt
     3121 gagcttcgag cccatcccca tccactactg cacacccgcc ggcttcgcca tcctgaagtg
     3181 caaggacaag aagttcaacg gcaccggccc ctgcaagaac gtgagcaccg tgcagtgcac
     3241 acatggcatc cggcccgtgg tgtccaccca gctgctcctg aatggaagcc tggccgagga
     3301 agaggtggtg atccgcagca gcaacttcac cgacaacgcc aagaacatca tcgtgcagct
     3361 gaaggagagc gtggagatca actgcacccg gcccaacaac aatacacgca agagcatcca
     3421 catcggaccc ggacgcgcct tctacaccac aggagagatc atcggagata tccgccaggc
     3481 ccactgcaac atcagccgca ccaagtggaa caacaccctg aaccagatcg ccaccaagct
     3541 gaaggagcag ttcggcaaca acaagaccat cgtgttcaac cagagcagcg gcggcgaccc
     3601 agagatcgtg atgcacagct tcaactgcgg cggcgagttc ttctactgca atagcaccca
     3661 gctgttcaac agcacctgga acttcaacgg cacatggaac ctgacccaga gcaacggcac
     3721 cgagggcaac gacaccatca cactgccatg ccgcatcaag cagatcatca acatgtggca
     3781 ggaggtgggc aaggccatgt acgctccacc catccgcggc cagattcgct gcagcagcaa
     3841 catcaccggc ctgatcctga cacgcgacgg cggcaccaac agcagcggca gcgagatctt
     3901 tcggccaggc ggaggcgaca tgcgggacaa ctggcggagc gagctgtaca agtacaaggt
     3961 ggtgaagatc gagcccctgg gagtcgctcc aaccaaggcc aaacgccggg tcgtgcagcg
     4021 cgagaagcgg gccgtgggca ccatcggagc catgttcctg ggattcctgg gcgccgccgg
     4081 aagcacaatg ggagctgcta gcatcaccct gaccgtgcag gctcgcctgc tcctgagcgg
     4141 catcgtgcag caacagaaca acctcctgcg cgctatcgag gcccagcaac acctgctcca
     4201 gctgaccgtg tggggcatca agcaactgca ggctcgcgtg ctggccctgg agcggtatct
     4261 gcgggatcag caactgctgg gcatctgggg ctgctccggc aagctgatct gcaccaccgc
     4321 cgtgccatgg aacgcgtctt ggagtaataa aactctggat atgatttgga ataacatgac
     4381 ctggatggag tgggaaagag aaatcgacaa ctacacaggc ttaatataca cattaattga
     4441 agaatcgcag aaccagcaag aaaagaatga acaagaatta ttagaattag ataagtgggc
     4501 aagtttgtgg aattggtttg acataacaaa ttggctgtgg tatataaaaa tattcataat
     4561 gatagtagga ggcttgatag gtttaagaat agtttttact gtactttcta tagtaaatag
     4621 agttaggcag ggatactcac cattgtcatt tcagacccac ctcccagccc cgaggggacc
     4681 cgacaggccc gaaggaatcg aagaagaagg tggagacaga gacagagaca gatccgtgcg
     4741 attagtggat ggattcttag cacttttctg ggacgacctg cggagcctgt gcctcttcag
     4801 ctaccaccgc ttgagagact tactcttgat tgtagcgagg attgtggaac ttctgggacg
     4861 cagggggtgg gaagccctca agtattggtg gaatctcctg cagtattgga gtcaggaact
     4921 aaggaatagt gctgttagct tgcttaatgc cacagctata gcagtagctg aggggacaga
     4981 tagggttata gaaatagtac aaagaattta tagggctatt ctccacatac ctacaagaat
     5041 aagacagggc ttggaaaggc ttttgctata atctagagaa ttctaccggg taggggaggc
     5101 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5161 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5221 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5281 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5341 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5401 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5461 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     5521 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5581 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     5641 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5701 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5761 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5821 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5881 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5941 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6001 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6061 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6121 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6181 tgcatgaccc gcaagcccgg tgcctgacgg gcgcgtctgg aacaatcaac ctctggatta
     6241 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6301 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6361 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6421 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6481 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6541 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6601 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6661 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6721 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6781 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     6841 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6901 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6961 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     7021 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     7081 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     7141 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     7201 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7261 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     7321 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     7381 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     7441 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     7501 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7561 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7621 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7681 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg
     7741 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9901 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
     9961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10081 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10141 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    10201 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
