LOCUS       sequence-381361-        4563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF674 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF674
FEATURES             Location/Qualifiers
     source          1..4563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1874..1973)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1990..2009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1991..2009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2461..3252
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3345..3933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3834..3853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4087..4104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4263..4290)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4382..4468)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4517..4539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4531..4548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4532..4548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gcccaccatg
      121 gccatgtccc aggaatcatt gaccttcaag gacgtgtttg tggacttcac cctggaggag
      181 tggcagcaac tggactctgc ccagaagaac ctctacaggg atgtcatgct tgagaactac
      241 agccacctgg tgtccgtggg gcatctagtt gggaagccag atgtgatctt caggttggga
      301 ccaggtgacg agtcctggat ggcagatgga gggaccccgg tacggacctg tgcaggtgag
      361 gacaggccag aagtctggga agttgacgag cagatagatc actacaagga aagccaagac
      421 aaatttctgt ggcaagctgc attcataggc aaggaaacac tgaaggatga aagtggtcaa
      481 gaatgtaaaa tatgtagaaa aatcatttat ctcaacacag actttgtttc tgtaaaacaa
      541 agactcccta aatattattc atgggaaagg tgttcaaaac atcatttaaa ctttcttggt
      601 caaaatagaa gctatgtaag aaagaaagat gatggatgta aggcatattg gaaagtatgc
      661 ctccattata atcttcataa agctcaacct gcagagagat tttttgaccc taatcaacga
      721 gggaaagccc tccaccaaaa gcaagccctt agaaaaagtc agagaagtca aactggggag
      781 aaactctaca aatgtactga atgtggaaaa gtgtttatcc agaaagcaaa cttagttgta
      841 catcaaagaa ctcacaccgg agagaaacct tatgaatgct gcgaatgtgc aaaagccttc
      901 agccagaagt caaccctcat agcacaccag agaactcaca caggggagaa gccctatgaa
      961 tgcagtgaat gtggaaaaac ctttatccag aagtcaactc tgattaaaca tcaacgaact
     1021 catacaggag agaaaccatt tgtatgtgac aaatgtccaa aagcctttaa gagttcatat
     1081 catcttatta gacatgaaaa aacacacatt agacaagcat tttataaagg tattaaatgt
     1141 actacgtcca gccttatata tcaaagaatt cacacaagtg agaaacctca gtgcagtgaa
     1201 catgggaaag cctctgatga gaagcccagt cccactaaac attggagaac tcatacaaaa
     1261 gagaacattt atgagtgtag taaatgtggg aaaagcttca gaggaaagtc acacctctct
     1321 gttcatcaga gaattcatac aggagagaaa ccctatgaat gtagtatatg tgggaagact
     1381 ttcagtggaa agtcacacct ctctgtccat catagaactc atacaggaga gaaaccttat
     1441 gaatgcagaa gatgtgggaa agcctttggg gagaagtcaa cccttattgt acatcagaga
     1501 atgcatacag gagagaaacc ctataaatgc aacgaatgtg ggaaagcctt tagtgagaag
     1561 tcaccactga ttaaacatca gagaattcat acaggagaga gaccctatga atgcactgac
     1621 tgtaaaaaag ccttcagtag gaagtcaact ctcatcaaac atcagagaat tcatacagga
     1681 gaaaaacctt acaaatgtag tgaatgtggg aaggccttca gtgtgaaatc aactctcatt
     1741 gtgcatcaca gaactcatac aggagagaaa ccttatgaat gcagagactg tgggaaagca
     1801 ttcagtggga agtcaactct cattaaacat cagagaagtc acacaggaga taaaaaccta
     1861 tcaggcctca tgggcccagc tttcttgtac aaagttggca ttataaaaaa taattgctca
     1921 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1981 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2041 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     2101 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     2161 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     2221 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2281 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2341 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2401 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     2461 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2521 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2581 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2641 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     2701 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     2761 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2821 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2881 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     2941 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3001 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3061 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3121 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     3181 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     3241 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3301 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3481 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3961 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4021 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4081 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4141 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4201 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4261 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4321 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4381 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4441 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4501 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4561 ccc
//
