LOCUS       sequence-381362-        5067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-OCA2_STOP
FEATURES             Location/Qualifiers
     source          1..5067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2624..2723)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2740..2759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2741..2759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2764..2780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2882..2901)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2893..3702
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2960..2979)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3849..4437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4338..4357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4591..4608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4767..4794)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4886..4972)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5021..5043
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5035..5052
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5036..5052
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgca cctggaggga
      121 agggacggcc ggagataccc tggagcacca gcagtggagc tgctgcagac cagcgtgcct
      181 tccggactgg cagagctggt ggcaggcaag aggcgcctgc caaggggagc aggaggagca
      241 gatcctagcc actcctgccc acgcggcgcc gccggccaga gctcctgggc cccagccggc
      301 caggagttcg cctcctttct gacaaagggc cggtctcact ctagcctgcc acagatgtcc
      361 tctagcagat ctaaggacag ctgcttcacc gagaacacac ccctgctgcg gaatagcctg
      421 caggagaagg gctccagatg tatcccagtg taccaccccg agtttatcac cgccgaggag
      481 agctgggagg actcctctgc cgattgggag cggagatatc tgctgtctag ggaggtgagc
      541 ggactgtctg ccagcgccag ctccgagaag ggcgacctgc tggatagccc ccacatccgg
      601 ctgagactgt ccaagctgag gcgctgcgtg cagtggctga aagtgatggg cctgttcgcc
      661 ttcgtggtgc tgtgctccat cctgttctct ctgtaccctg accagggcaa gctgtggcag
      721 ctgctggccc tgtctccact ggagaactat agcgtgaatc tgtctagcca cgtggactct
      781 acactgctgc aggtggatct ggcaggcgcc ctggtggcat ccggaccatc tcggcctggc
      841 agagaggagc acatcgtggt ggagctgacc caggcagatg cactgggatc caggtggcgg
      901 agaccccagc aggtgacaca caactggacc gtgtacctga atcctaggcg cagcgagcac
      961 tccgtgatgt ctaggacatt tgaggtgctg acccgcgaga cagtgtccat ctctatcagg
     1021 gcatctctgc agcagaccca ggcagtgcct ctgctgatgg cacaccagta cctgagaggc
     1081 agcgtggaga cccaggtgac aatcgcaacc gcaatcctgg caggcgtgta tgccctgatc
     1141 atcttcgaga tcgtgcacag gacactggcc gccatgctgg gatctctggc cgccctggcc
     1201 gccctggccg tgatcggcga caggccaagc ctgacacacg tggtggagtg gatcgatttc
     1261 gagaccctgg ccctgctgtt tggcatgatg atcctggtgg ccatctttag cgagaccggc
     1321 ttctttgact actgcgccgt gaaggcctat aggctgtcca ggggccgcgt gtgggcaatg
     1381 atcatcatgc tgtgcctgat cgcagccgtg ctgagcgcct tcctggacaa cgtgaccaca
     1441 atgctgctgt ttacaccagt gaccatcagg ctgtgcgagg tgctgaacct ggatccccgc
     1501 caggtgctga tcgccgaagt gatcttcacc aatatcggag gagcagcaac cgcaatcggc
     1561 gaccccccta acgtgatcat cgtgagcaat caggagctgc ggaagatggg cctggatttc
     1621 gccggcttta ccgcccacat gttcatcggc atctgcctgg tgctgctggt gtgctttccc
     1681 ctgctgcggc tgctgtactg gaacagaaag ctgtataata aggagccttc cgagatcgtg
     1741 gagctgaagc acgagatcca cgtgtggagg ctgacagccc agcgcatcag ccctgcatcc
     1801 agagaggaga ccgccgtgcg gagactgctg ctgggcaagg tgctggccct ggagcacctg
     1861 ctggcaaggc gcctgcacac attccaccgg cagatctccc aggaggacaa gaactgggag
     1921 accaatatcc aggagctgca gaagaagcac agaatctctg atggcatcct gctggccaag
     1981 tgtctgaccg tgctgggctt cgtgatcttt atgttctttc tgaatagctt tgtgcccggc
     2041 atccacctgg atctgggctg gatcgccatc ctgggcgcca tctggctgct gatcctggcc
     2101 gacatccacg atttcgagat catcctgcac agggtggagt gggccacact gctgttcttt
     2161 gccgccctgt ttgtgctgat ggaggccctg gcccacctgc acctgatcga gtacgtgggc
     2221 gagcagaccg ccctgctgat caagatggtg cctgaggagc agcgcctgat cgcagccatc
     2281 gtgctggtgg tgtgggtgag cgccctggca tcctctctga tcgacaacat cccattcacc
     2341 gccacaatga tccccgtgct gctgaatctg tcccacgatc cagaagtggg actgcctgca
     2401 ccaccactga tgtatgcact ggcctttggc gcatgcctgg gaggaaacgg cacactgatc
     2461 ggagcatccg ccaacgtggt gtgcgcagga atcgcagagc agcacggcta cggcttctcc
     2521 tttatggagt tctttagact gggcttcccc atgatggtgg tgtcttgcac cgtgggcatg
     2581 tgctatctgc tggtggcaca cgtggtggtg ggatggaact aagacccagc tttcttgtac
     2641 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     2701 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2761 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2821 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2881 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     2941 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3001 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3061 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3121 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3181 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3241 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3301 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3361 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3421 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3481 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3541 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3601 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3661 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3721 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3781 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3961 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4621 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4681 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     4741 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     4801 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     4861 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     4921 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     4981 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     5041 acgacggcca gtcttaagct cgggccc
//
