LOCUS       sequence-381364-        6238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC4A3_STOP
FEATURES             Location/Qualifiers
     source          1..6238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3806..3905)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3922..3941)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3923..3941)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3946..3962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3946..3962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4064..4083)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4075..4884
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4142..4161)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5031..5619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5520..5539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5762..5779
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5938..5965)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6057..6143)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6192..6214
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6206..6223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6207..6223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caacggcgtg
      121 atcccacctc caggaggagc aagcccactg cctcaggtgc gggtgcccct ggaggagccc
      181 cctctgtccc ctgacgtgga ggaggaggac gatgacctgg gcaagaccct ggccgtgtct
      241 agatttggcg atctgatcag caagccacca gcatgggacc cagagaagcc ttctcggagc
      301 tattccgaga gagatttcga gtttcacagg cacacaagcc accacaccca ccacccactg
      361 tccgccaggc tgcctccacc ccacaagctg cggagactgc ctccaacatc cgccaggcac
      421 accaggcgca agcgcaagaa ggagaagaca tccgccccac cttctgaggg aaccccacca
      481 atccaggagg agggaggagc aggcgtggac gaggaagaag aggaagaaga agaggaggag
      541 ggagagagcg aggcagagcc tgtggagcct ccacactccg gaaccccaca gaaggccaag
      601 ttctctatcg gcagcgacga ggatgacagc ccaggactgc caggaagggc agcagtgaca
      661 aagccactgc cctctgtggg cccacacacc gataagagcc cccagcacag ctcctctagc
      721 cctagcccaa gggcaagggc atccaggctg gcaggcgaga agtctagacc ttggtctcca
      781 agcgcctcct acgacctgag agagaggctg tgcccaggaa gcgccctggg aaacccagga
      841 ggccctgagc agcaggtgcc tacagatgag gcagaggcac agatgctggg atccgccgac
      901 ctggatgaca tgaagtctca caggctggag gataatccag gcgtgcggag acacctggtg
      961 aagaagccaa gcaggaccca gggaggaagg ggatctccaa gcggactggc accaatcctg
     1021 aggcggaaga agaagaagaa gaagctggac cggagacccc acgaggtgtt cgtggagctg
     1081 aacgagctga tgctggatcg ctctcaggag cctcactggc gggagacagc cagatggatc
     1141 aagtttgagg aggacgtgga ggaggagacc gagaggtggg gcaagcctca cgtggcctcc
     1201 ctgtctttcc gcagcctgct ggagctgagg aggaccatcg cacacggagc cgccctgctg
     1261 gatctggagc agaccacact gccaggcatc gcccacctgg tggtggagac aatgatcgtg
     1321 tctgatcaga tcaggcctga ggacagggca agcgtgctga ggaccctgct gctgaagcac
     1381 agccacccaa acgatgacaa ggactccggc ttctttccac gcaatccctc ctctagctcc
     1441 atgaactccg tgctgggcaa tcaccacccc acaccttctc acggacctga tggagcagtg
     1501 ccaaccatgg cagatgacct gggagagcca gcaccactgt ggcctcacga tccagacgca
     1561 aaggagaagc cactgcacat gcctggaggc gacggacaca ggggcaagtc cctgaagctg
     1621 ctggagaaga tccccgagga tgcagaggca acagtggtgc tggtgggatg cgtgcccttt
     1681 ctggagcagc ctgcagcagc cttcgtgcgg ctgaatgagg ccgtgctgct ggagagcgtg
     1741 ctggaggtgc ctgtgccagt gagattcctg tttgtgatgc tgggcccttc ccacacctct
     1801 acagactatc acgagctggg ccggtctatc gccaccctga tgagcgataa gctgtttcac
     1861 gaggccgcct accaggccga tgacagacag gatctgctga gcgccatctc cgagttcctg
     1921 gacggctcca tcgtgatccc accttctgag gtggagggaa gggacctgct gagatccgtg
     1981 gcagcctttc agagggagct gctgcgcaag cggagagaga gggagcagac aaaggtggag
     2041 atgaccacaa ggggaggata taccgcacca ggcaaggagc tgagcctgga gctgggagga
     2101 tccgaggcca caccagagga tgacccactg ctgaggaccg gatccgtgtt cggaggactg
     2161 gtgagagatg tgaggcgccg gtacccccac tatccttctg atctgaggga cgcactgcac
     2221 agccagtgcg tggccgccgt gctgttcatc tactttgccg ccctgagccc cgccatcaca
     2281 tttggcggac tgctgggaga gaagaccgag ggactgatgg gcgtgtctga gctgatcgtg
     2341 agcaccgccg tgctgggcgt gctgttctcc ctgctgggag cacagccact gctggtggtg
     2401 ggcttctctg gacctctgct ggtgtttgag gaggccttct ttaagttctg tagggcccag
     2461 gacctggagt atctgacagg acgcgtgtgg gtgggactgt ggctggtggt gttcgtgctg
     2521 gccctggtgg cagcagaggg atcctttctg gtgcggtaca tctctccatt cacccaggag
     2581 atcttcgcct ttctgatcag cctgatcttc atctatgaga ccttctacaa gctgtataag
     2641 gtgtttaccg agcacccact gctgcccttc tacccacccg agggcgccct ggagggaagc
     2701 ctggacgcag gcctggagcc taacggatcc gccctgcctc caaccgaggg acccccttct
     2761 cctagaaacc agccaaatac agccctgctg tccctgatcc tgatgctggg caccttcttt
     2821 atcgccttct ttctgcgcaa gttccggaat tctagatttc tgggcggcaa ggccagaagg
     2881 atcatcggcg actttggcat ccccatcagc atcctggtca tggtgctggt ggattactcc
     2941 atcaccgaca catataccca gaagctgaca gtgccaaccg gcctgtctgt gaccagcccc
     3001 gataagcggt cctggtttat cccacccctg ggctctgcca gacctttccc tccatggatg
     3061 atggtggcag cagcagtgcc agccctgctg gtgctgatcc tgatcttcat ggagacacag
     3121 atcaccgccc tgatcgtgtc tcagaaggca cgccggctgc tgaagggaag cggctttcac
     3181 ctggacctgc tgctgatcgg aagcctggga ggactgtgcg gactgttcgg actgccatgg
     3241 ctgacagcag caaccgtgcg gtccgtgaca cacgtgaatg ccctgacagt gatgagaacc
     3301 gccatcgccc ccggcgataa gcctcagatc caggaggtga gggagcagag ggtgaccggc
     3361 gtgctgatcg catccctggt gggactgtct atcgtgatgg gcgccgtgct gagaaggatc
     3421 ccactggccg tgctgttcgg catctttctg tatatgggcg tgacaagcct gtccggcatc
     3481 cagctgtccc agaggctgct gctgatcctg atgcccgcca agcaccaccc tgagcagcca
     3541 tacgtgacaa aggtgaagac ctggcgcatg cacctgttca cctgcatcca gctgggctgt
     3601 atcgccctgc tgtgggtggt gaagagcaca gccgcctccc tggccttccc ctttctgctg
     3661 ctgctgaccg tgcctctgag acactgtctg ctgccacggc tgtttcagga cagagagctg
     3721 caggccctgg atagcgagga cgcagagcca aacttcgatg aggacggcca ggatgagtac
     3781 aatgagctgc acatgcccgt gtaagaccca gctttcttgt acaaagttgg cattataaga
     3841 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     3901 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     3961 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     4021 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     4081 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     4141 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     4201 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     4261 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     4321 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     4381 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     4441 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     4501 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     4561 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     4621 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     4681 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     4741 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     4801 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     4861 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     4921 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     4981 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5161 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5341 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5641 ttacggttcc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5701 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5761 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5821 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5881 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
     5941 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
     6001 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
     6061 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
     6121 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
     6181 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
