LOCUS       sequence-381386-        9750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express wild-type beta-globin genes bi-directionally under 
            ponasteron A inducible promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Sato H, Singer RH
  TITLE     Cellular variability of nonsense-mediated mRNA decay.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.
  PUBMED    34893608
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7203. doi"; pages: " 
            10.1038/s41467-021-27423-0"
COMMENT     Sequence Label: PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)
FEATURES             Location/Qualifiers
     source          1..9750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1672..1910
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    4628..4709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4720..4737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4891..5479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4971..4990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5650..6441,6442..6510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6273..6292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6511..6615)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6648..6729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9349..9587)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 aagagaacaa tgcacttgtc catcgagcta acgagaacaa tgcacttagc ggtatcgaga
       61 acaatgcact taatatgcgg ccgagatccg tactccgacc cgggtactga gctttcagca
      121 agagaacaat gcacttgtcc atcgagcttt cagcaagaga acaatgcact tgtccatcga
      181 gctttcagca agagaacaat gcacttgtcc atcgagctaa cgagaacaat gcacttagcg
      241 gtatcgagaa caatgcactt aatatgcggc cgagatccgt actccgaccc gggtactgag
      301 ctttcagcaa gagaacaatg cacttgtcca tcgagctttc agcaagagaa caatgcactt
      361 gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctaac gagaacaatg
      421 cacttagcgg tatcgagaac aatgcactta atatgcggcc gagatccgta ctccgacccg
      481 ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca gcaagagaac
      541 aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat cgagctaacg
      601 agaacaatgc acttagcggt atcgagaaca atgcacttaa tatgcggccg agatccgtac
      661 tccgacccgg gtactgagct ttcagcaaga gaacaatgca cttgtccatc gagctttcag
      721 caagagaaca atgcacttgt ccatcgagct ttcagcaaga gaacaatgca cttgtccatc
      781 gagctaacga gaacaatgca cttagcggta tcgagaacaa tgcacttaat atgcggccga
      841 gatccgtact ccgacccggg tactgagctt tcagcaagag aacaatgcac ttgtccatcg
      901 agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag aacaatgcac
      961 ttgtccatcg agctaacgag aacaatgcac ttagcggtat cgagaacaat gcacttaata
     1021 tgcggccgag atccgtactc cgacccgggt actgagcttt cagcaagaga acaatgcact
     1081 tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt cagcaagaga
     1141 acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc gagaacaatg
     1201 cacttaatat gcggccgaga tccgtactcc gacccgggta ctgagctttc agcaagagaa
     1261 caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
     1321 agcaagagaa caatgcactt gtccatcgag ctaacgagaa caatgcactt agcggtatcg
     1381 agaacaatgc acttaatatg cggccgagat ccgtactccg acccgggtac tgagctttca
     1441 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
     1501 cgagctttca gcaagagaac aatgcacttg tccatcgagc taacgagaac aatgcactta
     1561 gcggtatcga gaacaatgca cttaatatgc ggccgagatc tgaattactg ccccgcccct
     1621 agcgattagc cccggccccg catagctccg ccccgggagt accctcgacc gccggagtat
     1681 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     1741 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     1801 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     1861 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gacaactctg aatactttca
     1921 aaagttaccg agaaagaaga actcagacac catatgccat ggtgcacctg actcctgagg
     1981 agaagtctgc cgttactgcc ctgtggggca aggtgaacgt ggatgaagtt ggtggtgagg
     2041 ccctgggcag gttggtatca aggttacaag acaggtttaa ggagaccaat agaaactggg
     2101 catgtggaga cagagaagac tcttgggttt ctgataggca ctgactctct ctgcctattg
     2161 gtctattttc ccacccttag gctgctggtg gtctaccctt ggacccagag gttctttgag
     2221 tcctttgggg atctgtccac tcctgatgct gttatgggca accctaaggt gaaggctcat
     2281 ggcaagaaag tgctcggtgc ctttagtgat ggcctggctc acctggacaa cctcaagggc
     2341 acctttgcca cactgagtga gctgcactgt gacaagctgc acgtggatcc tgagaacttc
     2401 agggtgagtc tgatgggcac ctcctgggtt tccttcccct ggctattctg ctcaaccttc
     2461 ctatcagaaa aaaaggggaa gcgattctag ggagcagtct ccatgactgt gtgtggagtg
     2521 ttgacaagag ttcggatatt ttattctcta ctcagaattg ctgctccccc tcactctgtt
     2581 ctgtgttgtc atttcctctt tctttggtaa gcttttaatt tccagttgca ttttactaaa
     2641 ttaattaagc tggttattta cttcccatcc tgatatcagc ttcccctcct cctttcctcc
     2701 cagtccttct ctctctcctc tctctttctc taatcctttc ctttccctca gttcatttct
     2761 tcttctttga tctacgtttg tttgtctttt taaatattgc cttgtaactt gctcagagga
     2821 caaggaagat atgtccctgt ttcttctcat agctctcaag aatagtagca taattggctt
     2881 ttatgccagg gtgacagggg aagaatatat tttacatata aattctgttt gacataggat
     2941 tcttataata atttgtcagt agtttaaggt tgcaaacaaa tgtctttgta aataagcctg
     3001 cagtatctgg tatttttgct ctacagttat gttgatggtt cttccatatt cccacagctc
     3061 ctgggcaata tgatcgtgat tgtgctgggc caccacctgg gcaaggattt cacccccgct
     3121 gcacaggctg ccttccagaa ggtggtggct ggagtggcca ctgccttggc tcacaagtac
     3181 cactaaaccc acgcgtgcta gatcccaata ccggtaacct acaaacgggt ggaggatcac
     3241 cccacccgac acttcacaat caaggggtac aatacacaag ggtggaggaa caccccaccc
     3301 tccagacaca ttacacagaa atccaatcaa acagaagcac catcagggct tctgctacca
     3361 aatttatctc aaaaaactac aacaaggaat caccatcagg gattccctgt gcaatatacg
     3421 tcaaacgagg gccacgacgg gaggacgatc acgcctcccg aatatcggca tgtctggctt
     3481 tcgaattcag tgcgtggagc atcagcccac gcagccaatc agagtcgaat acaagtcgac
     3541 tttcgcgaag agcatcagcc ttcgcgccat tcttacacaa accacactct cccctacagg
     3601 aacagcatca gcgttcctgc ccagtaccca actcaagaaa atttatgtcc ccatgcagca
     3661 tcagcgcatg ggccccaaga atacatcccc aacaaaatca catccgagca ccaacagggc
     3721 tcggagtgtt gtttcttgtc caactggaca aaccctccat ggaccatcag gccatggact
     3781 ctcaccaaca agacaaaaac tactcttctc gaagcagcat cagcgcttcg aaacactcga
     3841 gcatacattg tgcctatttc ttgggtggac gatcacgcca cccatgctct cacgaatttc
     3901 aaaacacgga caaggacgag caccaccagg gctcgtcgtt ccacgtccaa tacgattact
     3961 tacctttcgg gatcacgatc acggatcccg cagctacatc acttccactc aggacattca
     4021 agcatgcacg atcacggcat gctccacaag tctcaaccac agaaactacc aaatgggttc
     4081 agcaccagcg aacccactcc tacctcaaac ctcttcccac aaaactggca agcaggatca
     4141 ccgcttgccc attccaacat accaaatcaa aaacaattac tggtacagca tcagcgtacc
     4201 agcccacatc tctcactact atcaaaaacc aaaccgttca gcaacagcga acggtacaca
     4261 cggaaaaatc aactggttta caaatacgaa agacgatcac gctttcgtcc agcgcaaact
     4321 attacgaaaa acatccgacg ggaagagcaa cagccttccc gcggcggaaa acctcacaaa
     4381 aacacgacaa acggatgcac gaacacggca tccgccgaca acccacaaac ttacaaccag
     4441 gcaaacggtg caggatcacc gcaccgtaca tcaaacacct cagatctcat atcgataagc
     4501 ttgtcgacga tatctccaga ggatcataat cagccatacc acatttgtag aggttttact
     4561 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4621 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4681 tttcacaaat aaagcatttt tttcactgcc ccgagcttcc tcgctcactg actcgctgcg
     4741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6241 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     6301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcggca gtgaaaaaaa
     6661 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6721 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     6781 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcactag
     6841 tttgtatctc tgatggacgc aggtcgccat atcgactctg cgttgaagag taagaagtat
     6901 tcacaatagg aacgagtagt aggggtcgcg ccatatcgct gaccctttga tgatagtctc
     6961 tcagcacttc gaggcatgct gtggctaccc agcgagccca tatgctctgc tgggatgaat
     7021 gatggatgtc acttccagcc tgatgtagtc ggagggagcg aggccatatc ctctgctcct
     7081 ttgattggag aagatcaggt ccactgtaat gacagtggag ccagcgagcc catatgctct
     7141 gctggcttga agattggaca gtgacagcac atctgaagat gctgaggcac cgaggccata
     7201 tcctctggtg ccgatagact gcagcctcat aacccaatat cgagaataca taatagagat
     7261 ctgctctcgc ggacccatat gtcctgcgag atatcgaaca attttgtact tgcgtgtatt
     7321 ctcgacaaat accgacgacc ccatacggtc tgtcgggata cataacagtt gtaaatatat
     7381 ggacagggac agggaatcca gccaccccat acggtgtgct ggtaagtagt cgcgggtaca
     7441 caaactgatt tttgtacata accggagcct ccccatatgg agtgctcctc cgtaactgcg
     7501 cacagagcat aatactggtt gtatgattgg cctcggagcc atatctcctg aggcacgcgt
     7561 tcccaaatct tttgggtatg tatatacaga cacaacccag gagcccatac gctctctggg
     7621 accagataca agatagtaaa ggttcttggt cgacgatata aggacggagg ccataccctc
     7681 tcgtccgatg tatatacatt gccacgacat ttgggaacag tcggaagccg acgaggccat
     7741 atcctctgtc ggcgtatccc agtaataagc gctgagcgcg gtacaggtac agacggacga
     7801 ggccataccc tctgtccgtg tacaaaaagg cttggtaata gccgcgacat tctttttcgg
     7861 gagcgacccc atatggtctg ctcccatacc ctgaccttcg atgactgtct gatatgtata
     7921 catagcggag ggacccatat gtcctctccg cggaggtttt gtcgagaaac tggctttgta
     7981 caaaattggc tctcggcgag ccatatctcg tccgagaaat aaaagaagaa gtggatccca
     8041 taccggttac ccggtaccgg gtttagtggt acttgtgagc cagggcagtg gccactccag
     8101 ccaccacctt ctggaaggca gcctgtgcag cgggggtgaa atccttgccc aggtggtggc
     8161 ccagcacaat cacgatcata ttgcccagga gctgtgggaa tatggaagaa ccatcaacat
     8221 aactgtagag caaaaatacc agatactgca ggcttattta caaagacatt tgtttgcaac
     8281 cttaaactac tgacaaatta ttataagaat cctatgtcaa acagaattta tatgtaaaat
     8341 atattcttcc cctgtcaccc tggcataaaa gccaattatg ctactattct tgagagctat
     8401 gagaagaaac agggacatat cttccttgtc ctctgagcaa gttacaaggc aatatttaaa
     8461 aagacaaaca aacgtagatc aaagaagaag aaatgaactg agggaaagga aaggattaga
     8521 gaaagagaga ggagagagag aaggactggg aggaaaggag gaggggaagc tgatatcagg
     8581 atgggaagta aataaccagc ttaattaatt tagtaaaatg caactggaaa ttaaaagctt
     8641 accaaagaaa gaggaaatga caacacagaa cagagtgagg gggagcagca attctgagta
     8701 gagaataaaa tatccgaact cttgtcaaca ctccacacac agtcatggag actgctccct
     8761 agaatcgctt cccctttttt tctgatagga aggttgagca gaatagccag gggaaggaaa
     8821 cccaggaggt gcccatcaga ctcaccctga agttctcagg atccacgtgc agcttgtcac
     8881 agtgcagctc actcagtgtg gcaaaggtgc ccttgaggtt gtccaggtga gccaggccat
     8941 cactaaaggc accgagcact ttcttgccat gagccttcac cttagggttg cccataacag
     9001 catcaggagt ggacagatcc ccaaaggact caaagaacct ctgggtccaa gggtagacca
     9061 ccagcagcct aagggtggga aaatagacca ataggcagag agagtcagtg cctatcagaa
     9121 acccaagagt cttctctgtc tccacatgcc cagtttctat tggtctcctt aaacctgtct
     9181 tgtaaccttg ataccaacct gcccagggcc tcaccaccaa cttcatccac gttcaccttg
     9241 ccccacaggg cagtaacggc agacttctcc tcaggagtca ggtgcaccat gaattcaccg
     9301 gtgagctcgt gtctgagttc ttctttctcg gtaacttttg aaagtattca gagttgtctt
     9361 cttgtattca ataattactt cttggcagat ttcagtagtt gcagttgatt tacttggttg
     9421 ctggttactt ttaattgatt cactttaact tgcactttac tgcagattgt ttagcttgtt
     9481 cagctgcgct tgtttatttg cttagctttc gcttagcgac gtgttcactt tgcttgtttg
     9541 aattgaattg tcgctccgta gacgaagcgc ctctatttat actccggcgg tcgagggtac
     9601 tcccggggcg gagctatgcg gggccggggc taatcgctag gggcggggca gtactccgac
     9661 ccgggtactg agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag
     9721 aacaatgcac ttgtccatcg agctttcagc
//
