LOCUS       sequence-38141-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3357 (=HL 716 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    67..85
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(106..124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        192..296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             481..859
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(515..534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcattttt tttcctcctt agtcgacagt atctctatca ctgataggga
       61 tgtcaatctc tatcactgat agggactcga ggtgaagacg aaagggcctc gtgatacgcc
      121 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      901 ggcaacaatt aatagact
//
