LOCUS       sequence-381416-       11172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express wild-type and PTC-containing beta-globin genes 
            bi-directionally under ponasteron A inducible promoter in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Sato H, Singer RH
  TITLE     Cellular variability of nonsense-mediated mRNA decay.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.
  PUBMED    34893608
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7203. doi"; pages: " 
            10.1038/s41467-021-27423-0"
COMMENT     Sequence Label: pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)
FEATURES             Location/Qualifiers
     source          1..11172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1672..1910
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    4573..4654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4687..4791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4792..4860,4861..5652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5010..5029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5823..6411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6312..6331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6512..6633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6523..6542)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6763..7785)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(6763..7785)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(7793..7826)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     7934..7951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7962..8043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(10771..11009)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 aagagaacaa tgcacttgtc catcgagcta acgagaacaa tgcacttagc ggtatcgaga
       61 acaatgcact taatatgcgg ccgagatccg tactccgacc cgggtactga gctttcagca
      121 agagaacaat gcacttgtcc atcgagcttt cagcaagaga acaatgcact tgtccatcga
      181 gctttcagca agagaacaat gcacttgtcc atcgagctaa cgagaacaat gcacttagcg
      241 gtatcgagaa caatgcactt aatatgcggc cgagatccgt actccgaccc gggtactgag
      301 ctttcagcaa gagaacaatg cacttgtcca tcgagctttc agcaagagaa caatgcactt
      361 gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctaac gagaacaatg
      421 cacttagcgg tatcgagaac aatgcactta atatgcggcc gagatccgta ctccgacccg
      481 ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca gcaagagaac
      541 aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat cgagctaacg
      601 agaacaatgc acttagcggt atcgagaaca atgcacttaa tatgcggccg agatccgtac
      661 tccgacccgg gtactgagct ttcagcaaga gaacaatgca cttgtccatc gagctttcag
      721 caagagaaca atgcacttgt ccatcgagct ttcagcaaga gaacaatgca cttgtccatc
      781 gagctaacga gaacaatgca cttagcggta tcgagaacaa tgcacttaat atgcggccga
      841 gatccgtact ccgacccggg tactgagctt tcagcaagag aacaatgcac ttgtccatcg
      901 agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag aacaatgcac
      961 ttgtccatcg agctaacgag aacaatgcac ttagcggtat cgagaacaat gcacttaata
     1021 tgcggccgag atccgtactc cgacccgggt actgagcttt cagcaagaga acaatgcact
     1081 tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt cagcaagaga
     1141 acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc gagaacaatg
     1201 cacttaatat gcggccgaga tccgtactcc gacccgggta ctgagctttc agcaagagaa
     1261 caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
     1321 agcaagagaa caatgcactt gtccatcgag ctaacgagaa caatgcactt agcggtatcg
     1381 agaacaatgc acttaatatg cggccgagat ccgtactccg acccgggtac tgagctttca
     1441 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
     1501 cgagctttca gcaagagaac aatgcacttg tccatcgagc taacgagaac aatgcactta
     1561 gcggtatcga gaacaatgca cttaatatgc ggccgagatc tgaattactg ccccgcccct
     1621 agcgattagc cccggccccg catagctccg ccccgggagt accctcgacc gccggagtat
     1681 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     1741 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     1801 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     1861 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gacaactctg aatactttca
     1921 aaagttaccg agaaagaaga actcagacac catactgcgg ctagcatggt gcacctgact
     1981 cctgaggaga agtctgccgt tactgccctg tggggcaagg tgaacgtgga tgaagttggt
     2041 ggtgaggccc tgggcaggtt ggtatcaagg ttacaagaca ggtttaagga gaccaataga
     2101 aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg actctctctg
     2161 cctattggtc tattttccca cccttaggct gctggtggtc tacccttgga cctagaggtt
     2221 ctttgagtcc tttggggatc tgtccactcc tgatgctgtt atgggcaacc ctaaggtgaa
     2281 ggctcatggc aagaaagtgc tcggtgcctt tagtgatggc ctggctcacc tggacaacct
     2341 caagggcacc tttgccacac tgagtgagct gcactgtgac aagctgcacg tggatcctga
     2401 gaacttcagg gtgagtctga tgggcacctc ctgggtttcc ttcccctggc tattctgctc
     2461 aaccttccta tcagaaaaaa aggggaagcg attctaggga gcagtctcca tgactgtgtg
     2521 tggagtgttg acaagagttc ggatatttta ttctctactc agaattgctg ctccccctca
     2581 ctctgttctg tgttgtcatt tcctctttct ttggtaagct tttaatttcc agttgcattt
     2641 tactaaatta attaagctgg ttatttactt cccatcctga tatcagcttc ccctcctcct
     2701 ttcctcccag tccttctctc tctcctctct ctttctctaa tcctttcctt tccctcagtt
     2761 catttcttct tctttgatct acgtttgttt gtctttttaa atattgcctt gtaacttgct
     2821 cagaggacaa ggaagatatg tccctgtttc ttctcatagc tctcaagaat agtagcataa
     2881 ttggctttta tgccagggtg acaggggaag aatatatttt acatataaat tctgtttgac
     2941 ataggattct tataataatt tgtcagtagt ttaaggttgc aaacaaatgt ctttgtaaat
     3001 aagcctgcag tatctggtat ttttgctcta cagttatgtt gatggttctt ccatattccc
     3061 acagctcctg ggcaatatga tcgtgattgt gctgggccac cacctgggca aggatttcac
     3121 ccccgctgca caggctgcct tccagaaggt ggtggctgga gtggccactg ccctggctca
     3181 caagtaccac taaaccccgg taccgttgct tgctgaacac cgggtggtaa ccggtatggg
     3241 atccacttct tcttttattt ctcggacgag atatggctcg ccgagagcca attttgtaca
     3301 aagccagttt ctcgacaaaa cctccgcgga gaggacatat gggtccctcc gctatgtata
     3361 catatcagac agtcatcgaa ggtcagggta tgggagcaga ccatatgggg tcgctcccga
     3421 aaaagaatgt cgcggctatt accaagcctt tttgtacacg gacagagggt atggcctcgt
     3481 ccgtctgtac ctgtaccgcg ctcagcgctt attactggga tacgccgaca gaggatatgg
     3541 cctcgtcggc ttccgactgt tcccaaatgt cgtggcaatg tatatacatc ggacgagagg
     3601 gtatggcctc cgtccttata tcgtcgacca agaaccttta ctatcttgta tctggtccca
     3661 gagagcgtat gggctcctgg gttgtgtctg tatatacata cccaaaagat ttgggaacgc
     3721 gtgcctcagg agatatggct ccgaggccaa tcatacaacc agtattatgc tctgtgcgca
     3781 gttacggagg agcactccat atggggaggc tccggttatg tacaaaaatc agtttgtgta
     3841 cccgcgacta cttaccagca caccgtatgg ggtggctgga ttccctgtcc ctgtccatat
     3901 atttacaact gttatgtatc ccgacagacc gtatggggtc gtcggtattt gtcgagaata
     3961 cacgcaagta caaaattgtt cgatatctcg caggacatat gggtccgcga gagcagatct
     4021 ctattatgta ttctcgatat tgggttatga ggctgcagtc tatcggcacc agaggatatg
     4081 gcctcggtgc ctcagcatct tcagatgtgc tgtcactgtc caatcttcaa gccagcagag
     4141 catatgggct cgctggctcc actgtcatta cagtggacct gatcttctcc aatcaaagga
     4201 gcagaggata tggcctcgct ccctccgact acatcaggct ggaagtgaca tccatcattc
     4261 atcccagcag agcatatggg ctcgctgggt agccacagca tgcctcgaag tgctgagaga
     4321 ctatcatcaa agggtcagcg atatggcgcg acccctacta ctcgttccta ttgtgaatac
     4381 ttcttactct tcaacgcaga gtcgatatgg cgacctgcgt ccatcagaga tacaactttc
     4441 gtcgatccga aaaccatacg actagtgatc ataatcagcc ataccacatt tgtagaggtt
     4501 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4561 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4621 acaaatttca caaataaagc atttttttca ctgccgacgt caggtggcac ttttcgggga
     4681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4981 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgccaagct
     6481 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     6541 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6601 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     6661 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     6721 ttcatagaag gcggcggtgg aatcgaaatc tcgtagtacg tgctattcct ttgccctcgg
     6781 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     6841 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     6901 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     6961 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
     7021 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     7081 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     7141 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     7201 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     7261 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     7321 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     7381 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     7441 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     7501 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
     7561 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     7621 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     7681 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     7741 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttggcca aggaagttcc
     7801 tatactttct agagaatagg aacttcggaa taggaacatg ttctttcctg cgttatcccc
     7861 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7921 aacgaccgag cgcagcgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt
     7981 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     8041 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     8101 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagata
     8161 tcgtcgacaa gcttatcgat atgagatctg aggtgtttga tgtacggtgc ggtgatcctg
     8221 caccgtttgc ctggttgtaa gtttgtgggt tgtcggcgga tgccgtgttc gtgcatccgt
     8281 ttgtcgtgtt tttgtgaggt tttccgccgc gggaaggctg ttgctcttcc cgtcggatgt
     8341 ttttcgtaat agtttgcgct ggacgaaagc gtgatcgtct ttcgtatttg taaaccagtt
     8401 gatttttccg tgtgtaccgt tcgctgttgc tgaacggttt ggtttttgat agtagtgaga
     8461 gatgtgggct ggtacgctga tgctgtacca gtaattgttt ttgatttggt atgttggaat
     8521 gggcaagcgg tgatcctgct tgccagtttt gtgggaagag gtttgaggta ggagtgggtt
     8581 cgctggtgct gaacccattt ggtagtttct gtggttgaga cttgtggagc atgccgtgat
     8641 cgtgcatgct tgaatgtcct gagtggaagt gatgtagctg cgggatccgt gatcgtgatc
     8701 ccgaaaggta agtaatcgta ttggacgtgg aacgacgagc cctggtggtg ctcgtccttg
     8761 tccgtgtttt gaaattcgtg agagcatggg tggcgtgatc gtccacccaa gaaataggca
     8821 caatgtatgc tcgagtgttt cgaagcgctg atgctgcttc gagaagagta gtttttgtct
     8881 tgttggtgag agtccatggc ctgatggtcc atggagggtt tgtccagttg gacaagaaac
     8941 aacactccga gccctgttgg tgctcggatg tgattttgtt ggggatgtat tcttggggcc
     9001 catgcgctga tgctgcatgg ggacataaat tttcttgagt tgggtactgg gcaggaacgc
     9061 tgatgctgtt cctgtagggg agagtgtggt ttgtgtaaga atggcgcgaa ggctgatgct
     9121 cttcgcgaaa gtcgacttgt attcgactct gattggctgc gtgggctgat gctccacgca
     9181 ctgaattcga aagccagaca tgccgatatt cgggaggcgt gatcgtcctc ccgtcgtggc
     9241 cctcgtttga cgtatattgc acagggaatc cctgatggtg attccttgtt gtagtttttt
     9301 gagataaatt tggtagcaga agccctgatg gtgcttctgt ttgattggat ttctgtgtaa
     9361 tgtgtctgga gggtggggtg ttcctccacc cttgtgtatt gtaccccttg attgtgaagt
     9421 gtcgggtggg gtgatcctcc acccgtttgt aggttaccgg tattgggatc tagcacgcgt
     9481 gggtttagtg gtacttgtga gccaaggcag tggccactcc agccaccacc ttctggaagg
     9541 cagcctgtgc agcgggggtg aaatccttgc ccaggtggtg gcccagcaca atcacgatca
     9601 tattgcccag gagctgtggg aatatggaag aaccatcaac ataactgtag agcaaaaata
     9661 ccagatactg caggcttatt tacaaagaca tttgtttgca accttaaact actgacaaat
     9721 tattataaga atcctatgtc aaacagaatt tatatgtaaa atatattctt cccctgtcac
     9781 cctggcataa aagccaatta tgctactatt cttgagagct atgagaagaa acagggacat
     9841 atcttccttg tcctctgagc aagttacaag gcaatattta aaaagacaaa caaacgtaga
     9901 tcaaagaaga agaaatgaac tgagggaaag gaaaggatta gagaaagaga gaggagagag
     9961 agaaggactg ggaggaaagg aggaggggaa gctgatatca ggatgggaag taaataacca
    10021 gcttaattaa tttagtaaaa tgcaactgga aattaaaagc ttaccaaaga aagaggaaat
    10081 gacaacacag aacagagtga gggggagcag caattctgag tagagaataa aatatccgaa
    10141 ctcttgtcaa cactccacac acagtcatgg agactgctcc ctagaatcgc ttcccctttt
    10201 tttctgatag gaaggttgag cagaatagcc aggggaagga aacccaggag gtgcccatca
    10261 gactcaccct gaagttctca ggatccacgt gcagcttgtc acagtgcagc tcactcagtg
    10321 tggcaaaggt gcccttgagg ttgtccaggt gagccaggcc atcactaaag gcaccgagca
    10381 ctttcttgcc atgagccttc accttagggt tgcccataac agcatcagga gtggacagat
    10441 ccccaaagga ctcaaagaac ctctgggtcc aagggtagac caccagcagc ctaagggtgg
    10501 gaaaatagac caataggcag agagagtcag tgcctatcag aaacccaaga gtcttctctg
    10561 tctccacatg cccagtttct attggtctcc ttaaacctgt cttgtaacct tgataccaac
    10621 ctgcccaggg cctcaccacc aacttcatcc acgttcacct tgccccacag ggcagtaacg
    10681 gcagacttct cctcaggagt caggtgcacc atgaattcac cggtgagctc gtgtctgagt
    10741 tcttctttct cggtaacttt tgaaagtatt cagagttgtc ttcttgtatt caataattac
    10801 ttcttggcag atttcagtag ttgcagttga tttacttggt tgctggttac ttttaattga
    10861 ttcactttaa cttgcacttt actgcagatt gtttagcttg ttcagctgcg cttgtttatt
    10921 tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg
    10981 tagacgaagc gcctctattt atactccggc ggtcgagggt actcccgggg cggagctatg
    11041 cggggccggg gctaatcgct aggggcgggg cagtactccg acccgggtac tgagctttca
    11101 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
    11161 cgagctttca gc
//
