LOCUS       sequence-381418-       12637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP/mCherry linked wild-type and PTC-containing beta-globin 
            bi-directionally under ponasteron A inducible promoter in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Sato H, Singer RH
  TITLE     Cellular variability of nonsense-mediated mRNA decay.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.
  PUBMED    34893608
REFERENCE   2  (bases 1 to 12637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7203. doi"; pages: " 
            10.1038/s41467-021-27423-0"
COMMENT     Sequence Label: pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
FEATURES             Location/Qualifiers
     source          1..12637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1672..1910
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2011..2032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2272..2291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2619..2640
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5311..5392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5425..5529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5530..5598,5599..6390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5748..5767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6561..7149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7050..7069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7250..7371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7261..7280)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(7501..8523)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(7501..8523)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(8531..8564)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     8672..8689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8700..8781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11472..12179)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11747..11766)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     12016..12034
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(12236..12474)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 aagagaacaa tgcacttgtc catcgagcta acgagaacaa tgcacttagc ggtatcgaga
       61 acaatgcact taatatgcgg ccgagatccg tactccgacc cgggtactga gctttcagca
      121 agagaacaat gcacttgtcc atcgagcttt cagcaagaga acaatgcact tgtccatcga
      181 gctttcagca agagaacaat gcacttgtcc atcgagctaa cgagaacaat gcacttagcg
      241 gtatcgagaa caatgcactt aatatgcggc cgagatccgt actccgaccc gggtactgag
      301 ctttcagcaa gagaacaatg cacttgtcca tcgagctttc agcaagagaa caatgcactt
      361 gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctaac gagaacaatg
      421 cacttagcgg tatcgagaac aatgcactta atatgcggcc gagatccgta ctccgacccg
      481 ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca gcaagagaac
      541 aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat cgagctaacg
      601 agaacaatgc acttagcggt atcgagaaca atgcacttaa tatgcggccg agatccgtac
      661 tccgacccgg gtactgagct ttcagcaaga gaacaatgca cttgtccatc gagctttcag
      721 caagagaaca atgcacttgt ccatcgagct ttcagcaaga gaacaatgca cttgtccatc
      781 gagctaacga gaacaatgca cttagcggta tcgagaacaa tgcacttaat atgcggccga
      841 gatccgtact ccgacccggg tactgagctt tcagcaagag aacaatgcac ttgtccatcg
      901 agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag aacaatgcac
      961 ttgtccatcg agctaacgag aacaatgcac ttagcggtat cgagaacaat gcacttaata
     1021 tgcggccgag atccgtactc cgacccgggt actgagcttt cagcaagaga acaatgcact
     1081 tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt cagcaagaga
     1141 acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc gagaacaatg
     1201 cacttaatat gcggccgaga tccgtactcc gacccgggta ctgagctttc agcaagagaa
     1261 caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
     1321 agcaagagaa caatgcactt gtccatcgag ctaacgagaa caatgcactt agcggtatcg
     1381 agaacaatgc acttaatatg cggccgagat ccgtactccg acccgggtac tgagctttca
     1441 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
     1501 cgagctttca gcaagagaac aatgcacttg tccatcgagc taacgagaac aatgcactta
     1561 gcggtatcga gaacaatgca cttaatatgc ggccgagatc tgaattactg ccccgcccct
     1621 agcgattagc cccggccccg catagctccg ccccgggagt accctcgacc gccggagtat
     1681 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     1741 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     1801 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     1861 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gacaactctg aatactttca
     1921 aaagttaccg agaaagaaga actcagacac catactgcgg ctagcatggt gagcaagggc
     1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2401 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2461 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2581 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2641 accgccgccg ggatcactct cggcatggac gagctgtaca agtaccgatg cggccgctct
     2701 agcatggtgc acctgactcc tgaggagaag tctgccgtta ctgccctgtg gggcaaggtg
     2761 aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt acaagacagg
     2821 tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg ggtttctgat
     2881 aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc tggtggtcta
     2941 cccttggacc tagaggttct ttgagtcctt tggggatctg tccactcctg atgctgttat
     3001 gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta gtgatggcct
     3061 ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc actgtgacaa
     3121 gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct gggtttcctt
     3181 cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat tctagggagc
     3241 agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt ctctactcag
     3301 aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt ggtaagcttt
     3361 taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc catcctgata
     3421 tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct ttctctaatc
     3481 ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt ctttttaaat
     3541 attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt ctcatagctc
     3601 tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa tatattttac
     3661 atataaattc tgtttgacat aggattctta taataatttg tcagtagttt aaggttgcaa
     3721 acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca gttatgttga
     3781 tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc tgggccacca
     3841 cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg tggctggagt
     3901 ggccactgcc ctggctcaca agtaccacta aaccccggta ccgttgcttg ctgaacaccg
     3961 ggtggtaacc ggtatgggat ccacttcttc ttttatttct cggacgagat atggctcgcc
     4021 gagagccaat tttgtacaaa gccagtttct cgacaaaacc tccgcggaga ggacatatgg
     4081 gtccctccgc tatgtataca tatcagacag tcatcgaagg tcagggtatg ggagcagacc
     4141 atatggggtc gctcccgaaa aagaatgtcg cggctattac caagcctttt tgtacacgga
     4201 cagagggtat ggcctcgtcc gtctgtacct gtaccgcgct cagcgcttat tactgggata
     4261 cgccgacaga ggatatggcc tcgtcggctt ccgactgttc ccaaatgtcg tggcaatgta
     4321 tatacatcgg acgagagggt atggcctccg tccttatatc gtcgaccaag aacctttact
     4381 atcttgtatc tggtcccaga gagcgtatgg gctcctgggt tgtgtctgta tatacatacc
     4441 caaaagattt gggaacgcgt gcctcaggag atatggctcc gaggccaatc atacaaccag
     4501 tattatgctc tgtgcgcagt tacggaggag cactccatat ggggaggctc cggttatgta
     4561 caaaaatcag tttgtgtacc cgcgactact taccagcaca ccgtatgggg tggctggatt
     4621 ccctgtccct gtccatatat ttacaactgt tatgtatccc gacagaccgt atggggtcgt
     4681 cggtatttgt cgagaataca cgcaagtaca aaattgttcg atatctcgca ggacatatgg
     4741 gtccgcgaga gcagatctct attatgtatt ctcgatattg ggttatgagg ctgcagtcta
     4801 tcggcaccag aggatatggc ctcggtgcct cagcatcttc agatgtgctg tcactgtcca
     4861 atcttcaagc cagcagagca tatgggctcg ctggctccac tgtcattaca gtggacctga
     4921 tcttctccaa tcaaaggagc agaggatatg gcctcgctcc ctccgactac atcaggctgg
     4981 aagtgacatc catcattcat cccagcagag catatgggct cgctgggtag ccacagcatg
     5041 cctcgaagtg ctgagagact atcatcaaag ggtcagcgat atggcgcgac ccctactact
     5101 cgttcctatt gtgaatactt cttactcttc aacgcagagt cgatatggcg acctgcgtcc
     5161 atcagagata caactttcgt cgatccgaaa accatacgac tagtgatcat aatcagccat
     5221 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5281 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gccgacgtca
     5401 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5461 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5521 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5581 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5641 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5701 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5761 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5821 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5881 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5941 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6001 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6061 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6121 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6181 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6241 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6301 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6361 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6421 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6481 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6541 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6601 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6661 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6721 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6781 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6841 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6901 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6961 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7021 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7081 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7141 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7201 gctcacatgt gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt
     7261 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     7321 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc
     7381 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     7441 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagtacgtg
     7501 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     7561 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     7621 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     7681 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     7741 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     7801 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     7861 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     7921 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     7981 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     8041 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     8101 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     8161 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     8221 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     8281 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     8341 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     8401 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     8461 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     8521 tttggccaag gaagttccta tactttctag agaataggaa cttcggaata ggaacatgtt
     8581 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8641 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagctcgggg
     8701 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     8761 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     8821 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     8881 tatgatcctc tggagatatc gtcgacaagc ttatcgatat gagatctgag gtgtttgatg
     8941 tacggtgcgg tgatcctgca ccgtttgcct ggttgtaagt ttgtgggttg tcggcggatg
     9001 ccgtgttcgt gcatccgttt gtcgtgtttt tgtgaggttt tccgccgcgg gaaggctgtt
     9061 gctcttcccg tcggatgttt ttcgtaatag tttgcgctgg acgaaagcgt gatcgtcttt
     9121 cgtatttgta aaccagttga tttttccgtg tgtaccgttc gctgttgctg aacggtttgg
     9181 tttttgatag tagtgagaga tgtgggctgg tacgctgatg ctgtaccagt aattgttttt
     9241 gatttggtat gttggaatgg gcaagcggtg atcctgcttg ccagttttgt gggaagaggt
     9301 ttgaggtagg agtgggttcg ctggtgctga acccatttgg tagtttctgt ggttgagact
     9361 tgtggagcat gccgtgatcg tgcatgcttg aatgtcctga gtggaagtga tgtagctgcg
     9421 ggatccgtga tcgtgatccc gaaaggtaag taatcgtatt ggacgtggaa cgacgagccc
     9481 tggtggtgct cgtccttgtc cgtgttttga aattcgtgag agcatgggtg gcgtgatcgt
     9541 ccacccaaga aataggcaca atgtatgctc gagtgtttcg aagcgctgat gctgcttcga
     9601 gaagagtagt ttttgtcttg ttggtgagag tccatggcct gatggtccat ggagggtttg
     9661 tccagttgga caagaaacaa cactccgagc cctgttggtg ctcggatgtg attttgttgg
     9721 ggatgtattc ttggggccca tgcgctgatg ctgcatgggg acataaattt tcttgagttg
     9781 ggtactgggc aggaacgctg atgctgttcc tgtaggggag agtgtggttt gtgtaagaat
     9841 ggcgcgaagg ctgatgctct tcgcgaaagt cgacttgtat tcgactctga ttggctgcgt
     9901 gggctgatgc tccacgcact gaattcgaaa gccagacatg ccgatattcg ggaggcgtga
     9961 tcgtcctccc gtcgtggccc tcgtttgacg tatattgcac agggaatccc tgatggtgat
    10021 tccttgttgt agttttttga gataaatttg gtagcagaag ccctgatggt gcttctgttt
    10081 gattggattt ctgtgtaatg tgtctggagg gtggggtgtt cctccaccct tgtgtattgt
    10141 accccttgat tgtgaagtgt cgggtggggt gatcctccac ccgtttgtag gttaccggta
    10201 ttgggatcta gcacgcgtgg gtttagtggt acttgtgagc caaggcagtg gccactccag
    10261 ccaccacctt ctggaaggca gcctgtgcag cgggggtgaa atccttgccc aggtggtggc
    10321 ccagcacaat cacgatcata ttgcccagga gctgtgggaa tatggaagaa ccatcaacat
    10381 aactgtagag caaaaatacc agatactgca ggcttattta caaagacatt tgtttgcaac
    10441 cttaaactac tgacaaatta ttataagaat cctatgtcaa acagaattta tatgtaaaat
    10501 atattcttcc cctgtcaccc tggcataaaa gccaattatg ctactattct tgagagctat
    10561 gagaagaaac agggacatat cttccttgtc ctctgagcaa gttacaaggc aatatttaaa
    10621 aagacaaaca aacgtagatc aaagaagaag aaatgaactg agggaaagga aaggattaga
    10681 gaaagagaga ggagagagag aaggactggg aggaaaggag gaggggaagc tgatatcagg
    10741 atgggaagta aataaccagc ttaattaatt tagtaaaatg caactggaaa ttaaaagctt
    10801 accaaagaaa gaggaaatga caacacagaa cagagtgagg gggagcagca attctgagta
    10861 gagaataaaa tatccgaact cttgtcaaca ctccacacac agtcatggag actgctccct
    10921 agaatcgctt cccctttttt tctgatagga aggttgagca gaatagccag gggaaggaaa
    10981 cccaggaggt gcccatcaga ctcaccctga agttctcagg atccacgtgc agcttgtcac
    11041 agtgcagctc actcagtgtg gcaaaggtgc ccttgaggtt gtccaggtga gccaggccat
    11101 cactaaaggc accgagcact ttcttgccat gagccttcac cttagggttg cccataacag
    11161 catcaggagt ggacagatcc ccaaaggact caaagaacct ctgggtccaa gggtagacca
    11221 ccagcagcct aagggtggga aaatagacca ataggcagag agagtcagtg cctatcagaa
    11281 acccaagagt cttctctgtc tccacatgcc cagtttctat tggtctcctt aaacctgtct
    11341 tgtaaccttg ataccaacct gcccagggcc tcaccaccaa cttcatccac gttcaccttg
    11401 ccccacaggg cagtaacggc agacttctcc tcaggagtca ggtgcaccat gaattcaccg
    11461 gtgagctcgt gcttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
    11521 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
    11581 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
    11641 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
    11701 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
    11761 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
    11821 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
    11881 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
    11941 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
    12001 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
    12061 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
    12121 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
    12181 aattcactta agctcgtgtc tgagttcttc tttctcggta acttttgaaa gtattcagag
    12241 ttgtcttctt gtattcaata attacttctt ggcagatttc agtagttgca gttgatttac
    12301 ttggttgctg gttactttta attgattcac tttaacttgc actttactgc agattgttta
    12361 gcttgttcag ctgcgcttgt ttatttgctt agctttcgct tagcgacgtg ttcactttgc
    12421 ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc tatttatact ccggcggtcg
    12481 agggtactcc cggggcggag ctatgcgggg ccggggctaa tcgctagggg cggggcagta
    12541 ctccgacccg ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca
    12601 gcaagagaac aatgcacttg tccatcgagc tttcagc
//
