LOCUS       sequence-38142-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  TITLE     Linda Hicke lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP1481 - TOPO-ProteinA
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..269)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             complement(270..443)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     primer_bind     complement(560..579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(561..579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(586..603)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(586..602)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(595..617)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt cccaatgcgg
       61 ccgccggttg acttccccgc ggaattcgcg tctactttcg gcgcctgagc accatttagc
      121 tttttagctt ctgctaaaag gttagcgctt tggcttgggt catcttttaa actttggatg
      181 aaggcgtttc gttgttcttc gtttaagtta ggtaaatgta agatctcata gaacgcgttt
      241 tgttgttctt tgttgaattt gttgtctact ttcggcgcct gagcatcatt tagcttttta
      301 gcttctgcta aaaggttagc gctttggctt gggtcatctt ttaaactttg gatgaaggcg
      361 tttcgttgtt cttcgtttaa gttaggtaaa tgtaagatct catagaacgc gttttgttgt
      421 tctttgttga atttgttgtc cacggcttca tcgtgttgcg caagagccgc ggttttgcgg
      481 ccgcattggg aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
      541 atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
      601 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      661 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      721 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaancgc ggcgggtgtg
      781 gtggttactc gcagcgtgac cgctac
//
