LOCUS       sequence-381422-       12638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP/mCherry linked wild-type beta-globin bi-directionally 
            under ponasteron A inducible promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Sato H, Singer RH
  TITLE     Cellular variability of nonsense-mediated mRNA decay.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.
  PUBMED    34893608
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7203. doi"; pages: " 
            10.1038/s41467-021-27423-0"
COMMENT     Sequence Label: pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)
FEATURES             Location/Qualifiers
     source          1..12638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1672..1910
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2011..2032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2272..2291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2619..2640
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5312..5393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5426..5530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5531..5599,5600..6391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5749..5768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6562..7150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7051..7070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7251..7372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7262..7281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(7502..8524)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(7502..8524)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(8532..8565)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     8673..8690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8701..8782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11473..12180)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11748..11767)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     12017..12035
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(12237..12475)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 aagagaacaa tgcacttgtc catcgagcta acgagaacaa tgcacttagc ggtatcgaga
       61 acaatgcact taatatgcgg ccgagatccg tactccgacc cgggtactga gctttcagca
      121 agagaacaat gcacttgtcc atcgagcttt cagcaagaga acaatgcact tgtccatcga
      181 gctttcagca agagaacaat gcacttgtcc atcgagctaa cgagaacaat gcacttagcg
      241 gtatcgagaa caatgcactt aatatgcggc cgagatccgt actccgaccc gggtactgag
      301 ctttcagcaa gagaacaatg cacttgtcca tcgagctttc agcaagagaa caatgcactt
      361 gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctaac gagaacaatg
      421 cacttagcgg tatcgagaac aatgcactta atatgcggcc gagatccgta ctccgacccg
      481 ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca gcaagagaac
      541 aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat cgagctaacg
      601 agaacaatgc acttagcggt atcgagaaca atgcacttaa tatgcggccg agatccgtac
      661 tccgacccgg gtactgagct ttcagcaaga gaacaatgca cttgtccatc gagctttcag
      721 caagagaaca atgcacttgt ccatcgagct ttcagcaaga gaacaatgca cttgtccatc
      781 gagctaacga gaacaatgca cttagcggta tcgagaacaa tgcacttaat atgcggccga
      841 gatccgtact ccgacccggg tactgagctt tcagcaagag aacaatgcac ttgtccatcg
      901 agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag aacaatgcac
      961 ttgtccatcg agctaacgag aacaatgcac ttagcggtat cgagaacaat gcacttaata
     1021 tgcggccgag atccgtactc cgacccgggt actgagcttt cagcaagaga acaatgcact
     1081 tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt cagcaagaga
     1141 acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc gagaacaatg
     1201 cacttaatat gcggccgaga tccgtactcc gacccgggta ctgagctttc agcaagagaa
     1261 caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
     1321 agcaagagaa caatgcactt gtccatcgag ctaacgagaa caatgcactt agcggtatcg
     1381 agaacaatgc acttaatatg cggccgagat ccgtactccg acccgggtac tgagctttca
     1441 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
     1501 cgagctttca gcaagagaac aatgcacttg tccatcgagc taacgagaac aatgcactta
     1561 gcggtatcga gaacaatgca cttaatatgc ggccgagatc tgaattactg ccccgcccct
     1621 agcgattagc cccggccccg catagctccg ccccgggagt accctcgacc gccggagtat
     1681 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     1741 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     1801 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     1861 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gacaactctg aatactttca
     1921 aaagttaccg agaaagaaga actcagacac catactgcgg ctagcatggt gagcaagggc
     1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2401 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2461 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2581 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2641 accgccgccg ggatcactct cggcatggac gagctgtaca agtaccgatg cggccgctct
     2701 agcatggtgc acctgactcc tgaggagaag tctgccgtta ctgccctgtg gggcaaggtg
     2761 aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt acaagacagg
     2821 tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg ggtttctgat
     2881 aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc tggtggtcta
     2941 cccttggacc cagaggttct ttgagtcctt tggggatctg tccactcctg atgctgttat
     3001 gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta gtgatggcct
     3061 ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc actgtgacaa
     3121 gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct gggtttcctt
     3181 cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat tctagggagc
     3241 agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt ctctactcag
     3301 aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt ggtaagcttt
     3361 taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc catcctgata
     3421 tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct ttctctaatc
     3481 ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt ctttttaaat
     3541 attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt ctcatagctc
     3601 tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa tatattttac
     3661 atataaattc tgtttgacat aggattctta taataatttg tcagtagttt aaggttgcaa
     3721 acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca gttatgttga
     3781 tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc tgggccacca
     3841 cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg tggctggagt
     3901 ggccactgcc ctggctcaca agtaccacta aaccccggta ccgttgcttg ctgaacaccg
     3961 ggtggtaacc cggtatggga tccacttctt cttttatttc tcggacgaga tatggctcgc
     4021 cgagagccaa ttttgtacaa agccagtttc tcgacaaaac ctccgcggag aggacatatg
     4081 ggtccctccg ctatgtatac atatcagaca gtcatcgaag gtcagggtat gggagcagac
     4141 catatggggt cgctcccgaa aaagaatgtc gcggctatta ccaagccttt ttgtacacgg
     4201 acagagggta tggcctcgtc cgtctgtacc tgtaccgcgc tcagcgctta ttactgggat
     4261 acgccgacag aggatatggc ctcgtcggct tccgactgtt cccaaatgtc gtggcaatgt
     4321 atatacatcg gacgagaggg tatggcctcc gtccttatat cgtcgaccaa gaacctttac
     4381 tatcttgtat ctggtcccag agagcgtatg ggctcctggg ttgtgtctgt atatacatac
     4441 ccaaaagatt tgggaacgcg tgcctcagga gatatggctc cgaggccaat catacaacca
     4501 gtattatgct ctgtgcgcag ttacggagga gcactccata tggggaggct ccggttatgt
     4561 acaaaaatca gtttgtgtac ccgcgactac ttaccagcac accgtatggg gtggctggat
     4621 tccctgtccc tgtccatata tttacaactg ttatgtatcc cgacagaccg tatggggtcg
     4681 tcggtatttg tcgagaatac acgcaagtac aaaattgttc gatatctcgc aggacatatg
     4741 ggtccgcgag agcagatctc tattatgtat tctcgatatt gggttatgag gctgcagtct
     4801 atcggcacca gaggatatgg cctcggtgcc tcagcatctt cagatgtgct gtcactgtcc
     4861 aatcttcaag ccagcagagc atatgggctc gctggctcca ctgtcattac agtggacctg
     4921 atcttctcca atcaaaggag cagaggatat ggcctcgctc cctccgacta catcaggctg
     4981 gaagtgacat ccatcattca tcccagcaga gcatatgggc tcgctgggta gccacagcat
     5041 gcctcgaagt gctgagagac tatcatcaaa gggtcagcga tatggcgcga cccctactac
     5101 tcgttcctat tgtgaatact tcttactctt caacgcagag tcgatatggc gacctgcgtc
     5161 catcagagat acaactttcg tcgatccgaa aaccatacga ctagtgatca taatcagcca
     5221 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5281 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgccgacgtc
     5401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5761 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7201 tgctcacatg tgccaagctc tagctagagg tcgacggtat acagacatga taagatacat
     7261 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7321 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg
     7381 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     7441 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagtacgt
     7501 gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     7561 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     7621 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     7681 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     7741 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     7801 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     7861 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
     7921 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
     7981 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
     8041 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
     8101 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
     8161 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
     8221 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
     8281 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
     8341 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
     8401 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
     8461 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
     8521 ttttggccaa ggaagttcct atactttcta gagaatagga acttcggaat aggaacatgt
     8581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagctcggg
     8701 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     8761 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     8821 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     8881 ttatgatcct ctggagatat cgtcgacaag cttatcgata tgagatctga ggtgtttgat
     8941 gtacggtgcg gtgatcctgc accgtttgcc tggttgtaag tttgtgggtt gtcggcggat
     9001 gccgtgttcg tgcatccgtt tgtcgtgttt ttgtgaggtt ttccgccgcg ggaaggctgt
     9061 tgctcttccc gtcggatgtt tttcgtaata gtttgcgctg gacgaaagcg tgatcgtctt
     9121 tcgtatttgt aaaccagttg atttttccgt gtgtaccgtt cgctgttgct gaacggtttg
     9181 gtttttgata gtagtgagag atgtgggctg gtacgctgat gctgtaccag taattgtttt
     9241 tgatttggta tgttggaatg ggcaagcggt gatcctgctt gccagttttg tgggaagagg
     9301 tttgaggtag gagtgggttc gctggtgctg aacccatttg gtagtttctg tggttgagac
     9361 ttgtggagca tgccgtgatc gtgcatgctt gaatgtcctg agtggaagtg atgtagctgc
     9421 gggatccgtg atcgtgatcc cgaaaggtaa gtaatcgtat tggacgtgga acgacgagcc
     9481 ctggtggtgc tcgtccttgt ccgtgttttg aaattcgtga gagcatgggt ggcgtgatcg
     9541 tccacccaag aaataggcac aatgtatgct cgagtgtttc gaagcgctga tgctgcttcg
     9601 agaagagtag tttttgtctt gttggtgaga gtccatggcc tgatggtcca tggagggttt
     9661 gtccagttgg acaagaaaca acactccgag ccctgttggt gctcggatgt gattttgttg
     9721 gggatgtatt cttggggccc atgcgctgat gctgcatggg gacataaatt ttcttgagtt
     9781 gggtactggg caggaacgct gatgctgttc ctgtagggga gagtgtggtt tgtgtaagaa
     9841 tggcgcgaag gctgatgctc ttcgcgaaag tcgacttgta ttcgactctg attggctgcg
     9901 tgggctgatg ctccacgcac tgaattcgaa agccagacat gccgatattc gggaggcgtg
     9961 atcgtcctcc cgtcgtggcc ctcgtttgac gtatattgca cagggaatcc ctgatggtga
    10021 ttccttgttg tagttttttg agataaattt ggtagcagaa gccctgatgg tgcttctgtt
    10081 tgattggatt tctgtgtaat gtgtctggag ggtggggtgt tcctccaccc ttgtgtattg
    10141 taccccttga ttgtgaagtg tcgggtgggg tgatcctcca cccgtttgta ggttaccggt
    10201 attgggatct agcacgcgtg ggtttagtgg tacttgtgag ccaaggcagt ggccactcca
    10261 gccaccacct tctggaaggc agcctgtgca gcgggggtga aatccttgcc caggtggtgg
    10321 cccagcacaa tcacgatcat attgcccagg agctgtggga atatggaaga accatcaaca
    10381 taactgtaga gcaaaaatac cagatactgc aggcttattt acaaagacat ttgtttgcaa
    10441 ccttaaacta ctgacaaatt attataagaa tcctatgtca aacagaattt atatgtaaaa
    10501 tatattcttc ccctgtcacc ctggcataaa agccaattat gctactattc ttgagagcta
    10561 tgagaagaaa cagggacata tcttccttgt cctctgagca agttacaagg caatatttaa
    10621 aaagacaaac aaacgtagat caaagaagaa gaaatgaact gagggaaagg aaaggattag
    10681 agaaagagag aggagagaga gaaggactgg gaggaaagga ggaggggaag ctgatatcag
    10741 gatgggaagt aaataaccag cttaattaat ttagtaaaat gcaactggaa attaaaagct
    10801 taccaaagaa agaggaaatg acaacacaga acagagtgag ggggagcagc aattctgagt
    10861 agagaataaa atatccgaac tcttgtcaac actccacaca cagtcatgga gactgctccc
    10921 tagaatcgct tccccttttt ttctgatagg aaggttgagc agaatagcca ggggaaggaa
    10981 acccaggagg tgcccatcag actcaccctg aagttctcag gatccacgtg cagcttgtca
    11041 cagtgcagct cactcagtgt ggcaaaggtg cccttgaggt tgtccaggtg agccaggcca
    11101 tcactaaagg caccgagcac tttcttgcca tgagccttca ccttagggtt gcccataaca
    11161 gcatcaggag tggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc
    11221 accagcagcc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga
    11281 aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc
    11341 ttgtaacctt gataccaacc tgcccagggc ctcaccacca acttcatcca cgttcacctt
    11401 gccccacagg gcagtaacgg cagacttctc ctcaggagtc aggtgcacca tgaattcacc
    11461 ggtgagctcg tgcttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
    11521 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
    11581 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
    11641 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
    11701 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
    11761 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
    11821 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
    11881 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
    11941 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
    12001 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
    12061 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
    12121 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
    12181 gaattcactt aagctcgtgt ctgagttctt ctttctcggt aacttttgaa agtattcaga
    12241 gttgtcttct tgtattcaat aattacttct tggcagattt cagtagttgc agttgattta
    12301 cttggttgct ggttactttt aattgattca ctttaacttg cactttactg cagattgttt
    12361 agcttgttca gctgcgcttg tttatttgct tagctttcgc ttagcgacgt gttcactttg
    12421 cttgtttgaa ttgaattgtc gctccgtaga cgaagcgcct ctatttatac tccggcggtc
    12481 gagggtactc ccggggcgga gctatgcggg gccggggcta atcgctaggg gcggggcagt
    12541 actccgaccc gggtactgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
    12601 agcaagagaa caatgcactt gtccatcgag ctttcagc
//
