LOCUS       sequence-381425-       11173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express wild-type beta-globin genes bi-directionally under 
            ponasteron A inducible promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Sato H, Singer RH
  TITLE     Cellular variability of nonsense-mediated mRNA decay.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.
  PUBMED    34893608
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7203. doi"; pages: " 
            10.1038/s41467-021-27423-0"
COMMENT     Sequence Label: pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)
FEATURES             Location/Qualifiers
     source          1..11173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1672..1910
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    4574..4655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4688..4792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4793..4861,4862..5653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5011..5030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5824..6412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6313..6332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6513..6634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6524..6543)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6764..7786)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(6764..7786)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(7794..7827)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     7935..7952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7963..8044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(10772..11010)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 aagagaacaa tgcacttgtc catcgagcta acgagaacaa tgcacttagc ggtatcgaga
       61 acaatgcact taatatgcgg ccgagatccg tactccgacc cgggtactga gctttcagca
      121 agagaacaat gcacttgtcc atcgagcttt cagcaagaga acaatgcact tgtccatcga
      181 gctttcagca agagaacaat gcacttgtcc atcgagctaa cgagaacaat gcacttagcg
      241 gtatcgagaa caatgcactt aatatgcggc cgagatccgt actccgaccc gggtactgag
      301 ctttcagcaa gagaacaatg cacttgtcca tcgagctttc agcaagagaa caatgcactt
      361 gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctaac gagaacaatg
      421 cacttagcgg tatcgagaac aatgcactta atatgcggcc gagatccgta ctccgacccg
      481 ggtactgagc tttcagcaag agaacaatgc acttgtccat cgagctttca gcaagagaac
      541 aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat cgagctaacg
      601 agaacaatgc acttagcggt atcgagaaca atgcacttaa tatgcggccg agatccgtac
      661 tccgacccgg gtactgagct ttcagcaaga gaacaatgca cttgtccatc gagctttcag
      721 caagagaaca atgcacttgt ccatcgagct ttcagcaaga gaacaatgca cttgtccatc
      781 gagctaacga gaacaatgca cttagcggta tcgagaacaa tgcacttaat atgcggccga
      841 gatccgtact ccgacccggg tactgagctt tcagcaagag aacaatgcac ttgtccatcg
      901 agctttcagc aagagaacaa tgcacttgtc catcgagctt tcagcaagag aacaatgcac
      961 ttgtccatcg agctaacgag aacaatgcac ttagcggtat cgagaacaat gcacttaata
     1021 tgcggccgag atccgtactc cgacccgggt actgagcttt cagcaagaga acaatgcact
     1081 tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt cagcaagaga
     1141 acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc gagaacaatg
     1201 cacttaatat gcggccgaga tccgtactcc gacccgggta ctgagctttc agcaagagaa
     1261 caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca tcgagctttc
     1321 agcaagagaa caatgcactt gtccatcgag ctaacgagaa caatgcactt agcggtatcg
     1381 agaacaatgc acttaatatg cggccgagat ccgtactccg acccgggtac tgagctttca
     1441 gcaagagaac aatgcacttg tccatcgagc tttcagcaag agaacaatgc acttgtccat
     1501 cgagctttca gcaagagaac aatgcacttg tccatcgagc taacgagaac aatgcactta
     1561 gcggtatcga gaacaatgca cttaatatgc ggccgagatc tgaattactg ccccgcccct
     1621 agcgattagc cccggccccg catagctccg ccccgggagt accctcgacc gccggagtat
     1681 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     1741 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     1801 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     1861 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gacaactctg aatactttca
     1921 aaagttaccg agaaagaaga actcagacac catactgcgg ctagcatggt gcacctgact
     1981 cctgaggaga agtctgccgt tactgccctg tggggcaagg tgaacgtgga tgaagttggt
     2041 ggtgaggccc tgggcaggtt ggtatcaagg ttacaagaca ggtttaagga gaccaataga
     2101 aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg actctctctg
     2161 cctattggtc tattttccca cccttaggct gctggtggtc tacccttgga cccagaggtt
     2221 ctttgagtcc tttggggatc tgtccactcc tgatgctgtt atgggcaacc ctaaggtgaa
     2281 ggctcatggc aagaaagtgc tcggtgcctt tagtgatggc ctggctcacc tggacaacct
     2341 caagggcacc tttgccacac tgagtgagct gcactgtgac aagctgcacg tggatcctga
     2401 gaacttcagg gtgagtctga tgggcacctc ctgggtttcc ttcccctggc tattctgctc
     2461 aaccttccta tcagaaaaaa aggggaagcg attctaggga gcagtctcca tgactgtgtg
     2521 tggagtgttg acaagagttc ggatatttta ttctctactc agaattgctg ctccccctca
     2581 ctctgttctg tgttgtcatt tcctctttct ttggtaagct tttaatttcc agttgcattt
     2641 tactaaatta attaagctgg ttatttactt cccatcctga tatcagcttc ccctcctcct
     2701 ttcctcccag tccttctctc tctcctctct ctttctctaa tcctttcctt tccctcagtt
     2761 catttcttct tctttgatct acgtttgttt gtctttttaa atattgcctt gtaacttgct
     2821 cagaggacaa ggaagatatg tccctgtttc ttctcatagc tctcaagaat agtagcataa
     2881 ttggctttta tgccagggtg acaggggaag aatatatttt acatataaat tctgtttgac
     2941 ataggattct tataataatt tgtcagtagt ttaaggttgc aaacaaatgt ctttgtaaat
     3001 aagcctgcag tatctggtat ttttgctcta cagttatgtt gatggttctt ccatattccc
     3061 acagctcctg ggcaatatga tcgtgattgt gctgggccac cacctgggca aggatttcac
     3121 ccccgctgca caggctgcct tccagaaggt ggtggctgga gtggccactg ccctggctca
     3181 caagtaccac taaaccccgg taccgttgct tgctgaacac cgggtggtaa cccggtatgg
     3241 gatccacttc ttcttttatt tctcggacga gatatggctc gccgagagcc aattttgtac
     3301 aaagccagtt tctcgacaaa acctccgcgg agaggacata tgggtccctc cgctatgtat
     3361 acatatcaga cagtcatcga aggtcagggt atgggagcag accatatggg gtcgctcccg
     3421 aaaaagaatg tcgcggctat taccaagcct ttttgtacac ggacagaggg tatggcctcg
     3481 tccgtctgta cctgtaccgc gctcagcgct tattactggg atacgccgac agaggatatg
     3541 gcctcgtcgg cttccgactg ttcccaaatg tcgtggcaat gtatatacat cggacgagag
     3601 ggtatggcct ccgtccttat atcgtcgacc aagaaccttt actatcttgt atctggtccc
     3661 agagagcgta tgggctcctg ggttgtgtct gtatatacat acccaaaaga tttgggaacg
     3721 cgtgcctcag gagatatggc tccgaggcca atcatacaac cagtattatg ctctgtgcgc
     3781 agttacggag gagcactcca tatggggagg ctccggttat gtacaaaaat cagtttgtgt
     3841 acccgcgact acttaccagc acaccgtatg gggtggctgg attccctgtc cctgtccata
     3901 tatttacaac tgttatgtat cccgacagac cgtatggggt cgtcggtatt tgtcgagaat
     3961 acacgcaagt acaaaattgt tcgatatctc gcaggacata tgggtccgcg agagcagatc
     4021 tctattatgt attctcgata ttgggttatg aggctgcagt ctatcggcac cagaggatat
     4081 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     4141 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     4201 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     4261 catcccagca gagcatatgg gctcgctggg tagccacagc atgcctcgaa gtgctgagag
     4321 actatcatca aagggtcagc gatatggcgc gacccctact actcgttcct attgtgaata
     4381 cttcttactc ttcaacgcag agtcgatatg gcgacctgcg tccatcagag atacaacttt
     4441 cgtcgatccg aaaaccatac gactagtgat cataatcagc cataccacat ttgtagaggt
     4501 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4561 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4621 cacaaatttc acaaataaag catttttttc actgccgacg tcaggtggca cttttcgggg
     4681 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4741 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4981 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5101 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5341 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5401 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5641 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5701 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5761 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5821 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5881 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5941 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6001 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6061 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtgccaagc
     6481 tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac
     6541 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6601 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga
     6661 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     6721 tttcatagaa ggcggcggtg gaatcgaaat ctcgtagtac gtgctattcc tttgccctcg
     6781 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     6841 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     6901 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     6961 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     7021 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     7081 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     7141 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     7201 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     7261 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     7321 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     7381 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     7441 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     7501 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     7561 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     7621 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     7681 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     7741 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttggcc aaggaagttc
     7801 ctatactttc tagagaatag gaacttcgga ataggaacat gttctttcct gcgttatccc
     7861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7921 gaacgaccga gcgcagcgag tcagtgagcg aggaagctcg gggcagtgaa aaaaatgctt
     7981 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     8041 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     8101 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctggagat
     8161 atcgtcgaca agcttatcga tatgagatct gaggtgtttg atgtacggtg cggtgatcct
     8221 gcaccgtttg cctggttgta agtttgtggg ttgtcggcgg atgccgtgtt cgtgcatccg
     8281 tttgtcgtgt ttttgtgagg ttttccgccg cgggaaggct gttgctcttc ccgtcggatg
     8341 tttttcgtaa tagtttgcgc tggacgaaag cgtgatcgtc tttcgtattt gtaaaccagt
     8401 tgatttttcc gtgtgtaccg ttcgctgttg ctgaacggtt tggtttttga tagtagtgag
     8461 agatgtgggc tggtacgctg atgctgtacc agtaattgtt tttgatttgg tatgttggaa
     8521 tgggcaagcg gtgatcctgc ttgccagttt tgtgggaaga ggtttgaggt aggagtgggt
     8581 tcgctggtgc tgaacccatt tggtagtttc tgtggttgag acttgtggag catgccgtga
     8641 tcgtgcatgc ttgaatgtcc tgagtggaag tgatgtagct gcgggatccg tgatcgtgat
     8701 cccgaaaggt aagtaatcgt attggacgtg gaacgacgag ccctggtggt gctcgtcctt
     8761 gtccgtgttt tgaaattcgt gagagcatgg gtggcgtgat cgtccaccca agaaataggc
     8821 acaatgtatg ctcgagtgtt tcgaagcgct gatgctgctt cgagaagagt agtttttgtc
     8881 ttgttggtga gagtccatgg cctgatggtc catggagggt ttgtccagtt ggacaagaaa
     8941 caacactccg agccctgttg gtgctcggat gtgattttgt tggggatgta ttcttggggc
     9001 ccatgcgctg atgctgcatg gggacataaa ttttcttgag ttgggtactg ggcaggaacg
     9061 ctgatgctgt tcctgtaggg gagagtgtgg tttgtgtaag aatggcgcga aggctgatgc
     9121 tcttcgcgaa agtcgacttg tattcgactc tgattggctg cgtgggctga tgctccacgc
     9181 actgaattcg aaagccagac atgccgatat tcgggaggcg tgatcgtcct cccgtcgtgg
     9241 ccctcgtttg acgtatattg cacagggaat ccctgatggt gattccttgt tgtagttttt
     9301 tgagataaat ttggtagcag aagccctgat ggtgcttctg tttgattgga tttctgtgta
     9361 atgtgtctgg agggtggggt gttcctccac ccttgtgtat tgtacccctt gattgtgaag
     9421 tgtcgggtgg ggtgatcctc cacccgtttg taggttaccg gtattgggat ctagcacgcg
     9481 tgggtttagt ggtacttgtg agccaaggca gtggccactc cagccaccac cttctggaag
     9541 gcagcctgtg cagcgggggt gaaatccttg cccaggtggt ggcccagcac aatcacgatc
     9601 atattgccca ggagctgtgg gaatatggaa gaaccatcaa cataactgta gagcaaaaat
     9661 accagatact gcaggcttat ttacaaagac atttgtttgc aaccttaaac tactgacaaa
     9721 ttattataag aatcctatgt caaacagaat ttatatgtaa aatatattct tcccctgtca
     9781 ccctggcata aaagccaatt atgctactat tcttgagagc tatgagaaga aacagggaca
     9841 tatcttcctt gtcctctgag caagttacaa ggcaatattt aaaaagacaa acaaacgtag
     9901 atcaaagaag aagaaatgaa ctgagggaaa ggaaaggatt agagaaagag agaggagaga
     9961 gagaaggact gggaggaaag gaggagggga agctgatatc aggatgggaa gtaaataacc
    10021 agcttaatta atttagtaaa atgcaactgg aaattaaaag cttaccaaag aaagaggaaa
    10081 tgacaacaca gaacagagtg agggggagca gcaattctga gtagagaata aaatatccga
    10141 actcttgtca acactccaca cacagtcatg gagactgctc cctagaatcg cttccccttt
    10201 ttttctgata ggaaggttga gcagaatagc caggggaagg aaacccagga ggtgcccatc
    10261 agactcaccc tgaagttctc aggatccacg tgcagcttgt cacagtgcag ctcactcagt
    10321 gtggcaaagg tgcccttgag gttgtccagg tgagccaggc catcactaaa ggcaccgagc
    10381 actttcttgc catgagcctt caccttaggg ttgcccataa cagcatcagg agtggacaga
    10441 tccccaaagg actcaaagaa cctctgggtc caagggtaga ccaccagcag cctaagggtg
    10501 ggaaaataga ccaataggca gagagagtca gtgcctatca gaaacccaag agtcttctct
    10561 gtctccacat gcccagtttc tattggtctc cttaaacctg tcttgtaacc ttgataccaa
    10621 cctgcccagg gcctcaccac caacttcatc cacgttcacc ttgccccaca gggcagtaac
    10681 ggcagacttc tcctcaggag tcaggtgcac catgaattca ccggtgagct cgtgtctgag
    10741 ttcttctttc tcggtaactt ttgaaagtat tcagagttgt cttcttgtat tcaataatta
    10801 cttcttggca gatttcagta gttgcagttg atttacttgg ttgctggtta cttttaattg
    10861 attcacttta acttgcactt tactgcagat tgtttagctt gttcagctgc gcttgtttat
    10921 ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa ttgtcgctcc
    10981 gtagacgaag cgcctctatt tatactccgg cggtcgaggg tactcccggg gcggagctat
    11041 gcggggccgg ggctaatcgc taggggcggg gcagtactcc gacccgggta ctgagctttc
    11101 agcaagagaa caatgcactt gtccatcgag ctttcagcaa gagaacaatg cacttgtcca
    11161 tcgagctttc agc
//
