LOCUS       sequence-381442-        9651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries luciferase under CMV promoter flanked with IR/DR elements. 
            Also carries SB100X under PGK promoter outside of the IR/DR 
            elements..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Calinescu AA, Nunez FJ, Koschmann C, Kolb BL, Lowenstein PR, Castro 
            MG
  TITLE     Transposon mediated integration of plasmid DNA into the 
            subventricular zone of neonatal mice to generate novel models of 
            glioblastoma.
  JOURNAL   J Vis Exp. 2015 Feb 22;(96). doi: 10.3791/52443.
  PUBMED    25741859
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2015-02-22"; volume: "(96). doi"; pages: " 
            10.3791/52443"
COMMENT     Sequence Label: pT2/CMV-Luc/PGK-SB100X
FEATURES             Location/Qualifiers
     source          1..9651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     361..379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     539..558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     767..783
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        819..1318
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     1251..1270
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1357..2379
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    2396..2530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2420..2439)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2474..2493
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2773..2789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2799..2818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2799..2817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3427..3806
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3808..4083
                     /label=chicken beta-actin promoter
     intron          4084..5100
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5024..5046
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5108..5127
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5155..6807
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(5221..5239)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(6956..6975)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7021..7076
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7075..7094)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7779..7799)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7779..7797)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7818..7834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7818..7834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7831..7853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7842..7858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7866..7872,7873..7890,7891..7896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7911..7932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8049..8066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8220..8808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8300..8319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8979..9651,1..119,120..188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9602..9621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      361 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
      421 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      481 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      541 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      601 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
      661 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
      721 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
      781 agtgaattcg agctcggtac ccgggggcgc gccctaccgg gtaggggagg cgcttttccc
      841 aaggcagtct ggagcatgcg ctttagcggc cccgctgggc acttggcgct acacaagtgg
      901 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
      961 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     1021 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     1081 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     1141 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     1201 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     1261 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     1321 ctgcatccat ctagatcctt aattaagtct agtaccatgg gaaaatcaaa agaaatcagc
     1381 caagacctca gaaaaagaat tgtagacctc cacaagtctg gttcatcctt gggagcaatt
     1441 tccaaacgcc tggcggtacc acgttcatct gtacaaacaa tagtacgcaa gtataaacac
     1501 catgggacca cgcagccgtc ataccgctca ggaaggagac gcgttctgtc tcctagagat
     1561 gaacgtactt tggtgcgaaa agtgcaaatc aatcccagaa caacagcaaa ggaccttgtg
     1621 aagatgctgg aggaaacagg tacaaaagta tctatatcca cagtaaaacg agtcctatat
     1681 cgacataacc tgaaaggcca ctcagcaagg aagaagccac tgctccaaaa ccgacataag
     1741 aaagccagac tacggtttgc aactgcacat ggggacaaag atcgtacttt ttggagaaat
     1801 gtcctctggt ctgatgaaac aaaaatagaa ctgtttggcc ataatgacca tcgttatgtt
     1861 tggaggaaga agggggaggc ttgcaagccg aagaacacca tcccaaccgt gaagcacggg
     1921 ggtggcagca tcatgttgtg ggggtgcttt gctgcaggag ggactggtgc acttcacaaa
     1981 atagatggca tcatggacgc ggtgcagtat gtggatatat tgaagcaaca tctcaagaca
     2041 tcagtcagga agttaaagct tggtcgcaaa tgggtcttcc aacacgacaa tgaccccaag
     2101 catacttcca aagttgtggc aaaatggctt aaggacaaca aagtcaaggt attggagtgg
     2161 ccatcacaaa gccctgacct caatcctata gaaaatttgt gggcagaact gaaaaagcgt
     2221 gtgcgagcaa ggaggcctac aaacctgact cagttacacc agctctgtca ggaggaatgg
     2281 gccaaaattc acccaaatta ttgtgggaag cttgtggaag gctacccgaa acgtttgacc
     2341 caagttaaac aatttaaagg caatgctacc aaatactagg ggccctaacc gcggggatcc
     2401 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2461 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2521 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2581 ggaggttttt tcggatcctc tagagtcgac tgtttattaa cgcttacaat ttccattcgc
     2641 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2701 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2761 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     2821 aattggagct cggatcccta tacagttgaa gtcggaagtt tacatacact taagttggag
     2881 tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca atagttttgg
     2941 caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa caattgttta
     3001 cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc agaagtttac
     3061 atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat gatgtcatgg
     3121 ctttagaagc tagatctggc catctagagc ggccgcgcgc actagtgaat tccatggata
     3181 tcaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     3241 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     3301 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     3361 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctct
     3421 agagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     3481 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3541 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3601 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3661 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3721 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3781 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     3841 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     3901 cagcgatggg ggcggggggg gggggggsgc gcgccaggcg gggcggggcg gggcgagggg
     3961 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4021 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4081 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     4141 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4201 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     4261 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     4321 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     4381 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     4441 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     4501 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     4561 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     4621 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     4681 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     4741 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     4801 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     4861 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     4921 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     4981 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     5041 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     5101 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcatggaa
     5161 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct
     5221 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     5281 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
     5341 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     5401 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     5461 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
     5521 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
     5581 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     5641 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
     5701 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact
     5761 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
     5821 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     5881 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     5941 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
     6001 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
     6061 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
     6121 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
     6181 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     6241 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     6301 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
     6361 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     6421 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
     6481 aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc
     6541 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
     6601 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     6661 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     6721 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     6781 aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga cgaaattctt
     6841 agctattgta atactctagg ggggatccac tagaattcac tcctcaggtg caggctgcct
     6901 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     6961 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     7021 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     7081 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     7141 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     7201 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     7261 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     7321 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     7381 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttgtggaa ggctactcga
     7441 aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa ttgagtgtat
     7501 gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct
     7561 ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta
     7621 agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt
     7681 atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct ctagctagag
     7741 tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg
     7801 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7861 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7921 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7981 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaacc
     8401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9421 atcgttgtca gaagtaactt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa a
//
