LOCUS       sequence-381465-        9549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Streptococcus pyogenes Cas9 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Jacobus AP, Barreto JA, de Bem LS, Menegon YA, Fier I, Bueno JGR, 
            Dos Santos LV, Gross J
  TITLE     EasyGuide Plasmids Support in Vivo Assembly of gRNAs for CRISPR/Cas9
            Applications in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 18;11(11):3886-3891. doi: 
            10.1021/acssynbio.2c00348. Epub 2022 Oct 18.
  PUBMED    36257021
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00348"; journalName: "ACS Synth Biol"; date: 
            "2022-11-18- 18"; volume: "11"; issue: "11"; pages: "3886-3891"
COMMENT     Sequence Label: pEasyCas9
FEATURES             Location/Qualifiers
     source          1..9549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..1357
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1..344
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             345..1154
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(412..431)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1160..1357
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             2046..6149
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2046..6149
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6162..6182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6198..6445
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6214..6232)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(6456..7044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6536..6555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7215..8006,8007..8075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7838..7857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8076..8180)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8207..9549
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9170..9217)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
       61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
      181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      301 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga
      361 ctcacgtttc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
      421 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
      481 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      541 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      601 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc
      661 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      721 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
      781 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
      841 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
      901 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
      961 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1021 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1081 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     1141 atgagttttt ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     1201 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     1261 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     1321 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     1381 ccatccagtg tcgaaaacgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1441 cagcgtatta ccttctgctg gattcaaaca ctcttctcca gtaaaaagat tcccttctca
     1501 ctctgcttag agaaggagtg ccaggccggc taagcccact ctccagacgg aaaccataca
     1561 atgcctccgc tggctgcatt gtcgcgcccc gcccaacgac ggtttaccga cagctgctag
     1621 ctgggctcaa caggtggtta gcccaccaat tcccctgtcg ctcttcgctc tgaatgtgac
     1681 ggcaaatttc gacccgttgt tcctgttcct tttttttttc aattggactg aaaaaaaaaa
     1741 agaaccgaat ctggaaagat acacccaaac atacatagaa tgtacggatg catgattgtc
     1801 tcagcctcgt ttggctcatc gttcttcatt tctttttcct aattttgata gagacaatag
     1861 atagacgtgg aaggaaaaaa aaaaggaaag cccaacaata ttgagaaacg aagaggtgta
     1921 tttggtttaa atagagcctc ttcattcctt tcctgatctg acaacagggt ggaacataaa
     1981 atatagatct gtagtgagtg cgaatagcaa tagtaagtga acgaaaaagg aatacgataa
     2041 tacatatgga caagaagtac tctattggtt tggacattgg taccaactct gttggttggg
     2101 ctgttattac cgacgaatac aaggttccat ctaagaagtt caaggttttg ggtaacaccg
     2161 acagacactc tattaagaag aacttgattg gtgctttgtt gttcgactct ggtgaaaccg
     2221 ctgaagctac cagattgaag agaaccgcta gaagaagata caccagaaga aagaacagaa
     2281 tttgttactt gcaagaaatt ttctctaacg aaatggctaa ggttgacgac tctttcttcc
     2341 acagattgga agaatctttc ttggttgaag aagacaagaa gcacgaaaga cacccaattt
     2401 tcggtaacat tgttgacgaa gttgcttacc acgaaaagta cccaaccatt taccacttga
     2461 gaaagaagtt ggttgactct accgacaagg ctgacttgag attgatttac ttggctttgg
     2521 ctcacatgat taagttcaga ggtcacttct tgattgaagg tgacttgaac ccagacaact
     2581 ctgacgttga caagttgttc attcaattgg ttcaaaccta caaccaattg ttcgaagaaa
     2641 acccaattaa cgcttctggt gttgacgcta aggctatttt gtctgctaga ttgtctaagt
     2701 ctagaagatt ggaaaacttg attgctcaat tgccaggtga aaagaagaac ggtttgttcg
     2761 gtaacttgat tgctttgtct ttgggtttga ccccaaactt caagtctaac ttcgacttgg
     2821 ctgaagacgc taagttgcaa ttgtctaagg acacctacga cgacgacttg gacaacttgt
     2881 tggctcaaat tggtgaccaa tacgctgact tgttcttggc tgctaagaac ttgtctgacg
     2941 ctattttgtt gtctgacatt ttgagagtta acaccgaaat taccaaggct ccattgtctg
     3001 cttctatgat taagagatac gacgaacacc accaagactt gaccttgttg aaggctttgg
     3061 ttagacaaca attgccagaa aagtacaagg aaattttctt cgaccaatct aagaacggtt
     3121 acgctggtta cattgacggt ggtgcttctc aagaagaatt ctacaagttc attaagccaa
     3181 ttttggaaaa gatggacggt accgaagaat tgttggttaa gttgaacaga gaagacttgt
     3241 tgagaaagca aagaaccttc gacaacggtt ctattccaca ccaaattcac ttgggtgaat
     3301 tgcacgctat tttgagaaga caagaagact tctacccatt cttgaaggac aacagagaaa
     3361 agattgaaaa gattttgacc ttcagaattc catactacgt tggtccattg gctagaggta
     3421 actctagatt cgcttggatg accagaaagt ctgaagaaac cattacccca tggaacttcg
     3481 aagaagttgt tgacaagggt gcttctgctc aatctttcat tgaaagaatg accaacttcg
     3541 acaagaactt gccaaacgaa aaggttttgc caaagcactc tttgttgtac gaatacttca
     3601 ccgtttacaa cgaattgacc aaggttaagt acgttaccga aggtatgaga aagccagctt
     3661 tcttgtctgg tgaacaaaag aaggctattg ttgacttgtt gttcaagacc aacagaaagg
     3721 ttaccgttaa gcaattgaag gaagactact tcaagaagat tgaatgtttc gactctgttg
     3781 aaatttctgg tgttgaagac agattcaacg cttctttggg tacctaccac gacttgttga
     3841 agattattaa ggacaaggac ttcttggaca acgaagaaaa cgaagacatt ttggaagaca
     3901 ttgttttgac cttgaccttg ttcgaagaca gagaaatgat tgaagaaaga ttgaagacct
     3961 acgctcactt gttcgacgac aaggttatga agcaattgaa gagaagaaga tacaccggtt
     4021 ggggtagatt gtctagaaag ttgattaacg gtattagaga caagcaatct ggtaagacca
     4081 ttttggactt cttgaagtct gacggtttcg ctaacagaaa cttcatgcaa ttgattcacg
     4141 acgactcttt gaccttcaag gaagacattc aaaaggctca agtttctggt caaggtgact
     4201 ctttgcacga acacattgct aacttggctg gttctccagc tattaagaag ggtattttgc
     4261 aaaccgttaa ggttgttgac gaattggtta aggttatggg tagacacaag ccagaaaaca
     4321 ttgttattga aatggctaga gaaaaccaaa ccacccaaaa gggtcaaaag aactctagag
     4381 aaagaatgaa gagaattgaa gaaggtatta aggaattggg ttctcaaatt ttgaaggaac
     4441 acccagttga aaacacccaa ttgcaaaacg aaaagttgta cttgtactac ttgcaaaacg
     4501 gtagagacat gtacgttgac caagaattgg acattaacag attgtctgac tacgacgttg
     4561 accacattgt tccacaatct ttcttgaagg acgactctat tgacaacaag gttttgacca
     4621 gatctgacaa gaacagaggt aagtctgaca acgttccatc tgaagaagtt gttaagaaga
     4681 tgaagaacta ctggagacaa ttgttgaacg ctaagttgat tacccaaaga aagttcgaca
     4741 acttgaccaa ggctgaaaga ggtggtttgt ctgaattgga caaggctggt ttcattaaga
     4801 gacaattggt tgaaaccaga caaattacca agcacgttgc tcaaattttg gactctagaa
     4861 tgaacaccaa gtacgacgaa aacgacaagt tgattagaga agttaaggtt attaccttga
     4921 agtctaagtt ggtttctgac ttcagaaagg acttccaatt ctacaaggtt agagaaatta
     4981 acaactacca ccacgctcac gacgcttact tgaacgctgt tgttggtacc gctttgatta
     5041 agaagtaccc aaagttggaa tctgaattcg tttacggtga ctacaaggtt tacgacgtta
     5101 gaaagatgat tgctaagtct gaacaagaaa ttggtaaggc taccgctaag tacttcttct
     5161 actctaacat tatgaacttc ttcaagaccg aaattacctt ggctaacggt gaaattagaa
     5221 agagaccatt gattgaaacc aacggtgaaa ccggtgaaat tgtttgggac aagggtagag
     5281 acttcgctac cgttagaaag gttttgtcta tgccacaagt taacattgtt aagaagaccg
     5341 aagttcaaac cggtggtttc tctaaggaat ctattttgcc aaagagaaac tctgacaagt
     5401 tgattgctag aaagaaggac tgggacccaa agaagtacgg tggtttcgac tctccaaccg
     5461 ttgcttactc tgttttggtt gttgctaagg ttgaaaaggg taagtctaag aagttgaagt
     5521 ctgttaagga attgttgggt attaccatta tggaaagatc ttctttcgaa aagaacccaa
     5581 ttgacttctt ggaagctaag ggttacaagg aagttaagaa ggacttgatt attaagttgc
     5641 caaagtactc tttgttcgaa ttggaaaacg gtagaaagag aatgttggct tctgctggtg
     5701 aattgcaaaa gggtaacgaa ttggctttgc catctaagta cgttaacttc ttgtacttgg
     5761 cttctcacta cgaaaagttg aagggttctc cagaagacaa cgaacaaaag caattgttcg
     5821 ttgaacaaca caagcactac ttggacgaaa ttattgaaca aatttctgaa ttctctaaga
     5881 gagttatttt ggctgacgct aacttggaca aggttttgtc tgcttacaac aagcacagag
     5941 acaagccaat tagagaacaa gctgaaaaca ttattcactt gttcaccttg accaacttgg
     6001 gtgctccagc tgctttcaag tacttcgaca ccaccattga cagaaagaga tacacctcta
     6061 ccaaggaagt tttggacgct accttgattc accaatctat taccggtttg tacgaaacca
     6121 gaattgactt gtctcaattg ggtggtgact ctagagctga cccaaagaag aagagaaagg
     6181 tttaagtcga cctcgagtca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     6241 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     6301 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     6361 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     6421 gggacgctcg aaggctttaa tttgcggctg gcgtttttcc ataggctccg cccccctgac
     6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt
     8221 gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     8281 gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat
     8341 ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     8401 aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa
     8461 agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac
     8521 aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa
     8581 ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct
     8641 tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt
     8701 gcattttttc aagataaagg catccccgat tatattctat accgatgtgg attgcgcata
     8761 ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg
     8821 tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt
     8881 tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag
     8941 ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat
     9001 gggtaggtta tatagggata tagcacagag atatatagca aagagatact tttgagcaat
     9061 gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg
     9121 ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat
     9181 actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc
     9241 ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc
     9301 tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta
     9361 aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga
     9421 tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc
     9481 tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga
     9541 tattggatc
//
