LOCUS       sequence-381469-        6702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang 
            Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam
            P. Arkin
  TITLE     Acetaminophen production in the edible, filamentous cyanobacterium 
            Arthrospira platensis
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pJMH059
FEATURES             Location/Qualifiers
     source          1..6702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4481..4497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4481..4497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4494..4516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4505..4521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4529..4535,4536..4553,4554..4559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4574..4595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4712..4729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4883..5471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4963..4982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5642..6433,6434..6502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6265..6284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6503..6607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6675..6693
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggaat cttgtgcgaa ttgaggtaac
      421 ctcagatcct atccttgagg ttgattgcgt atttctgggg aaaacggagt ttcctcggcc
      481 ctataatgaa actgataagg aggaagattg atgcgaaaat acatcatttt tcgggctgat
      541 aagggtgaac ctggttggaa agaacgtaaa ctccaacata cccagggttt aactagaatt
      601 ttggctgaac atttcgactc ttctgatcaa cctattcctg agttgggata tcgaccgagg
      661 gagtttattc gtattgatgg gttgcataat cccactgaac atggctacag cacccattat
      721 cgtcagggtg actgggaagt aactagggta gaaagttact ctcctgatgt gtctatggct
      781 gagtttgata tgatcgtgat ttgcttttgc aaatatagcc ctgttaatgg tcctgtgcaa
      841 cctatgccag agcgtcgagt gtctgtagat gggttcgctg gggatgagaa gcggttggag
      901 caatggcacg agtcccaaaa gaaaccgact gaggtttagt tggtccccgg tttgcttatt
      961 tgactagcca gattcgccac gacaacagaa ccgccagcag ggaagagggg aatagaaccg
     1021 cgaaaagagc gatcgcattg gtttcgggaa tggcgaaatt tggcccgcca tatttgatca
     1081 gtaaggatag ccctagggat aggatcagga ttttggccac caataacagt ttgttgtcca
     1141 tttatatata ggatgcgatg ggatgcgatc gcctatattt taccaattgg tggcggcctt
     1201 aaccccctaa gccctatgtc tgacacccag tcaccccaac cgccttagtc ggtattcccc
     1261 actgggtggg cgatacacga tttcccggaa aaattgaaaa aaaattttgt ggcaggtgat
     1321 atgatttgcg atcgaggtgg gtaagttaga actacagaaa acaaataacc tcatttcacc
     1381 ccgaggagaa cgaattatga gagaagctgt gatcgccgag gtttcaacac agttatccga
     1441 agttgtaggg gttatcgaac gacatttaga gccaacctta ttggcggtac acttgtatgg
     1501 ttcagctgtc gatggaggcc ttaaacccca ttccgatatt gaccttctgg tcactgtgac
     1561 agttagactt gatgaaacca ctagaagggc cctgatcaat gacttattag aaacttctgc
     1621 aagtcctggt gaaagtgaga tattgagggc ggtagaagtc acaattgtcg tgcacgatga
     1681 cattatcccc tggagatacc ccgctaaaag ggaactgcaa tttggagagt ggcagcgaaa
     1741 cgatattcta gcaggcatat tcgaacctgc gactatcgat attgaccttg ctatactgtt
     1801 gacaaaagcc cgcgagcatt ctgtggcact agttggaccg gctgccgaag aattatttga
     1861 tcctgttccg gaacaagacc ttttcgaagc actgaatgag accctaactc tctggaacag
     1921 tccacccgat tgggccggag acgagcggaa tgttgtatta accttgagcc gaatctggta
     1981 ttcggcggtg actggcaaaa tagctccaaa agatgttgca gcggactggg ctatggaaag
     2041 gttacctgca caatatcagc cggttatact agaggcacgg caagcgtacc tcgggcagga
     2101 agaggatcgc ttagcttcaa gagccgacca attggaagag ttcgtacact acgtcaaagg
     2161 ggaaattacc aaagtcgtgg gtaaataatg tctaacaatt cgttcaagcc gacgccgctt
     2221 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     2281 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     2341 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
     2401 caacatccgc attaaaatct agcgagggct ttactaagct gatccgggct attggtgatc
     2461 tctcggcttt aaccagttgc gcccacttcc tagagtggcg ggatcgggat tattcatgat
     2521 ctggccgcta atatttacaa tacttaatat tatttttagt catttagtga tatatatgtt
     2581 ttttttggac caaatgtgtg gttttgcaaa gaaatataaa caaatgtaat ggaaatatta
     2641 acaatattaa aggttgactg aagccattta ttgatagtat tttaatcaat cggcaaatag
     2701 ccggaaattt aaagagcgat tcctcagaag ggagatataa aggagttatg gtatctaaag
     2761 gtgaagagct gtttaccgga gttgtaccca ttttagttga attggatggc gacgtaaacg
     2821 ggcataaatt ctcagtctcc ggggaaggag agggagatgc aacttatggc aaactaacac
     2881 tcaaatttat ctgtaccact gggaaactac cagtcccctg gcctactctc gtgacaacct
     2941 ttggctatgg gttacaatgc ttcgcaaggt atccagatca catgaaacag cacgactttt
     3001 tcaaatcagc gatgcctgaa ggctacgttc aagagcgaac aatatttttc aaagatgacg
     3061 gaaactacaa aacaagagcg gaagttaggt ttgagggtga taccctggta aaccgcatcg
     3121 aattgaaagg aatcgatttc aaagaggacg gtaacatact tggacataaa ctggaataca
     3181 actacaactc gcacaacgtc tacatcatgg ctgataaaca gaaaaatggt atcaaagtga
     3241 acttcaaaat cagacataac atcgaggatg ggtcagttca gttagccgac cactaccaac
     3301 agaatactcc gatcggtgat ggaccggttt tattgccaga caaccattat ctttcttacc
     3361 agagtgctct gagcaaagat cctaatgaaa aacgggacca catggtcctt ctggagtttg
     3421 tgaccgctgc cggcattact ctagggatgg atgaactcta taaataacag ataatctagc
     3481 agtttaggtt tcagatggag tcagctaggg tctttaaccc catcgtcaac tataagatgg
     3541 agagatggca tacggtgaca ctgttaaagc cataactata ttgctcttgt tgataaaatc
     3601 agaaactgag gccacttgag agtattgtaa ataggtagta cagtagtgct acctattttg
     3661 ttttaaaata actaattgag tttatcgctg ggactatggt tgataatcag atgtcggaaa
     3721 agcagaatat tttatcagca gaagctgata tttacttgca taaaagggat tatcaacaag
     3781 cagccatttt atatgaacaa gccatcaatc tcaatcccga ctataaatat aattactgga
     3841 aactggggtt aaccctgctg ctacaggagc gggaggaaga agcccagacc acctggctgg
     3901 tggcgatgag tgatggagac ccagaacaga ttgaccagtg gacgatagac ttactcgagg
     3961 tgctatcatc agaagccgat gcacgaacct ccgccgaaga ctattccgtg gcttggggga
     4021 ttcgccaaca cattttagaa ataaaccccc atggtattga taatatatta aagttaattg
     4081 atttgtcaat taccctgaca acctataccg gaaatgagat cgcagaatat ggactggttc
     4141 ccttactgca acaggagtcc atcaatgtca atccagattt actactgcac ctgtggaaaa
     4201 aactcctagt tgtagatgca attaatcccc tatctctgga attgggagag gcggtgatta
     4261 accatcttgg tgagcaaccg gagtttgtca ctagcttaat ccctaaatta tacgatattg
     4321 cctattcctt tggtgaaata ggtatagcca agggctatgg agagttgttg ttgaacctgg
     4381 caccgcatca tcaagagttg ctgagggctc tggctcaatt ttgtattcaa ttaaccgagt
     4441 atgaccatgg cattgaatat gccagactgg cgtaatcatg gtcatagctg tttcctgtgt
     4501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6601 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6661 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
