LOCUS       sequence-381532-        5335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CoVMab7 light chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5335)
  TITLE     Murrell lab nanobodies
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: hIgG1_kappa_CoVMab7
FEATURES             Location/Qualifiers
     source          1..5335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     20..37
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     51..70
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      827..836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    840..974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(877..896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     931..950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1043..1400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1043..1234
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1066..1086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1251..1386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1420..1437)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1420..1436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1429..1451)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1649..2104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1736..1755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1946..1967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2121..2140)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2240..2262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2300..2318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2386..2490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2491..2559,2560..3351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2709..2728)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3522..4110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4011..4030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4264..4281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4398..4419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4434..4439,4440..4457,4458..4464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4472..4488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4477..4499
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4496..4512
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4496..4512
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        4541..4920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4921..5124
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5075..5095
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5121..5145
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct
       61 ctccacaggt gtccactccc aggtccaact gcacctcggt tctatcgatt gaattccacc
      121 atgggatggt catgtatcat cctttttcta gtagcaactg caaccggtgt acactcgcaa
      181 attgttctca cccagtctcc agcactcatg tctgcatctc caggggagaa ggtcaccatg
      241 acctgcagtg ccagctcaag tgtaagttac atttactggt accagcagaa gccaagatcc
      301 tcccccaaac cctggattta tctcacatcc aacctggctt ctggagtccc tgctcgcttc
      361 agtggcagtg ggtctgggac ctcttactct ctcacaatca gcagcatgga ggctgaagat
      421 gctgccactt attactgcca gcagtggagt agtaacccat tcacgttcgg ctcggggaca
      481 aagttggaaa taaaacgtac ggtggctgca ccatctgtct tcatcttccc gccatctgat
      541 gagcagttga aatctggaac tgcctctgtt gtgtgcctgc tgaataactt ctatcccaga
      601 gaggccaaag tacagtggaa ggtggataac gccctccaat cgggtaactc ccaggagagt
      661 gtcacagagc aggacagcaa ggacagcacc tacagcctca gcagcaccct gacgctgagc
      721 aaagcagact acgagaaaca caaagtctac gcctgcgaag tcacccatca gggcctgagc
      781 tcgcccgtca caaagagctt caacagggga gagtgttaga agcttggccg ccatggccca
      841 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      901 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      961 atcatgtctg gatcgggaat taattcggcg cagcaccatg gcctgaaata acctctgaaa
     1021 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca tctgtggaat gtgtgtcagt
     1081 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1141 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     1201 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1261 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1321 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1381 gaggcctagg cttttgcaaa aagctgttaa cagcttggca ctggccgtcg ttttacaacg
     1441 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     1501 cgccagttgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     1561 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     1621 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     1681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1861 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1981 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     2101 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2281 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2701 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3001 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4321 gcgcgttggc cgattcatta atccaactgg cacgacaggt ttcccgactg gaaagcgggc
     4381 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4441 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4501 aacagctatg acatgattac gaattaattc gagctcgccc gacattgatt attgactagt
     4561 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4621 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4681 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4741 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4801 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4861 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4921 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4981 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5041 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     5101 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     5161 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
     5221 cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc
     5281 tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat aacct
//
