LOCUS       sequence-381534-        6098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CoVMab4 heavy chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  TITLE     Murrell lab nanobodies
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: hIgG1_heavy_CoVMab4
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     20..37
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     51..70
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1590..1599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1603..1737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1640..1659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1694..1713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1806..2163
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1806..1997
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1829..1849)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2014..2149
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2183..2200)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2183..2199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2192..2214)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2412..2867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2499..2518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2709..2730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2884..2903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3003..3025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3063..3081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3149..3253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3254..3322,3323..4114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3472..3491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4285..4873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4774..4793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5027..5044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5161..5182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5197..5202,5203..5220,5221..5227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5235..5251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5240..5262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5259..5275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5259..5275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5304..5683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5684..5887
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5838..5858
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5884..5908
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct
       61 ctccacaggt gtccactccc aggtccaact gcacctcggt tctatcgatt gaattccacc
      121 atgggatggt catgtatcat cctttttcta gtagcaactg caaccggtgt acactcgctg
      181 gtccaactgc agcagcctgg ggctgacctt gtgatgcctg gggcttcagt gaagctgtcc
      241 tgcaaggctt ctggctacac cttcatcagc tactggatgc actgggtgag gcagaggcct
      301 ggacaaggcc ttgagtggat cggagcgatt aatccttctg ataattatgt taactataat
      361 caaaagttca cagacaagtc cacattgact gtagacaaat cctccaacac agtctacatg
      421 cagctcagca gcctgacatc tgaggactct gcggtctatt attgtgcaag accccttgat
      481 ggttactacc cttacttaga ctactggggc caaggcacca ctctcacagt ctcctcagcg
      541 tcgaccaagg gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc
      601 acagcggccc tgggctgcct ggtcaaggac tacttccccg aacctgtgac ggtctcgtgg
      661 aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga
      721 ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac
      781 atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagagagt tgagcccaaa
      841 tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg
      901 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag
      961 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac
     1021 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc
     1081 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag
     1141 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa
     1201 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg
     1261 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc
     1321 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg
     1381 gactccgacg gctccttctt cctctatagc aagctcaccg tggacaagag caggtggcag
     1441 caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag
     1501 aagagcctct ccctgtcccc gggtaaatga gtgcgacggc cggcaagccc ccgctccccg
     1561 ggctctcgcg gtcgtacgag gaaagcttgg ccgccatggc ccaacttgtt tattgcagct
     1621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     1681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg
     1741 aattaattcg gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
     1801 accttctgag gcggaaagaa ccatctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     1861 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     1921 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1981 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2041 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2101 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     2161 aaaaagctgt taacagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2221 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagt tggcgtaata
     2281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2341 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     2401 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     2641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     2761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2821 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     2881 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2941 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3001 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3061 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3121 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5041 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5101 ttaatccaac tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5221 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     5281 tacgaattaa ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat
     5341 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     5401 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     5461 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     5521 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     5581 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     5641 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     5701 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     5761 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     5821 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     5881 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     5941 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
     6001 gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt
     6061 ggcttcgtta gaacgcggct acaattaata cataacct
//
