LOCUS       sequence-381536-        6077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CoVMab7 heavy chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6077)
  TITLE     Murrell lab nanobodies
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: hIgG1_heavy_CoVMab7
FEATURES             Location/Qualifiers
     source          1..6077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     20..37
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     51..70
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1569..1578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1582..1716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1619..1638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1673..1692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1785..2142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1785..1976
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1808..1828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1993..2128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2162..2179)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2162..2178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2171..2193)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2391..2846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2478..2497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2688..2709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2863..2882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2982..3004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3042..3060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3128..3232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3233..3301,3302..4093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3451..3470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4264..4852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4753..4772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5006..5023
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5140..5161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5176..5181,5182..5199,5200..5206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5214..5230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5219..5241
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5238..5254
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5238..5254
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5283..5662
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5663..5866
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5817..5837
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5863..5887
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct
       61 ctccacaggt gtccactccc aggtccaact gcacctcggt tctatcgatt gaattccacc
      121 atgggatggt catgtatcat cctttttcta gtagcaactg caaccggtgt acactcgcag
      181 gttcagctgc agcagtctgg ggctgagctg gtgaagcctg gggcctcagt gaagatttcc
      241 tgcaaagctt ctggctacgc attcattagg gactggatga actgggtgaa gcagaggcct
      301 ggaaagggtc ttgagtggat tggacagatt tatcctggag atggtgatac taactacaac
      361 ggaaagttca agggcaaggc cacactgact gcagacaaat cctccagcac agcctacatg
      421 cagctcagca ggttgacctc tgaggactct gcggtctatt tctgtgcaag atatttcgat
      481 gtctggggca cagggaccac ggtcaccgtc tcctcagcgt cgaccaaggg cccatcggtc
      541 ttccccctgg caccctcctc caagagcacc tctgggggca cagcggccct gggctgcctg
      601 gtcaaggact acttccccga acctgtgacg gtctcgtgga actcaggcgc cctgaccagc
      661 ggcgtgcaca ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg
      721 gtgaccgtgc cctccagcag cttgggcacc cagacctaca tctgcaacgt gaatcacaag
      781 cccagcaaca ccaaggtgga caagagagtt gagcccaaat cttgtgacaa aactcacaca
      841 tgcccaccgt gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca
      901 aaacccaagg acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac
      961 gtgagccacg aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat
     1021 aatgccaaga caaagccgcg ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc
     1081 ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac
     1141 aaagccctcc cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa
     1201 ccacaggtgt acaccctgcc cccatcccgg gaggagatga ccaagaacca ggtcagcctg
     1261 acctgcctgg tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg
     1321 cagccggaga acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc
     1381 ctctatagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc
     1441 tccgtgatgc atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtccccg
     1501 ggtaaatgag tgcgacggcc ggcaagcccc cgctccccgg gctctcgcgg tcgtacgagg
     1561 aaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
     1621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1681 tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg cgcagcacca
     1741 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     1801 catctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     1861 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     1921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     1981 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt aacagcttgg
     2161 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2221 gccttgcagc acatccccct ttcgccagtt ggcgtaatag cgaagaggcc cgcaccgatc
     2281 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     2341 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     2401 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2461 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2521 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2581 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     2641 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2701 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     2761 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     2821 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     2881 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2941 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3001 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3061 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5041 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatccaact ggcacgacag
     5101 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     5161 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5221 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattaat tcgagctcgc
     5281 ccgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     5341 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     5401 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     5461 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     5521 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     5581 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     5641 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     5701 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     5761 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     5821 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     5881 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     5941 atccagcctc cgcggccggg aacggtgcat tggaacgcgg attccccgtg ccaagagtga
     6001 cgtaagtacc gcctatagag tctataggcc cacccccttg gcttcgttag aacgcggcta
     6061 caattaatac ataacct
//
