LOCUS       sequence-381556-        4860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a 2X catalytic mutant of (ORF37) D221N E244Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Hartenian E, Mendez AS, Didychuk AL, Khosla S, Glaunsinger BA
  TITLE     DNA processing by the Kaposi's sarcoma-associated herpesvirus 
            alkaline exonuclease SOX contributes to viral gene expression and 
            infectious virion production.
  JOURNAL   Nucleic Acids Res. 2023 Jan 11;51(1):182-197. doi: 
            10.1093/nar/gkac1190.
  PUBMED    36537232
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-11- 11"; volume: "51"; issue: 
            "1"; pages: "182-197"
COMMENT     Sequence Label: pACE SOX (ORF37) D221N E244Q
FEATURES             Location/Qualifiers
     source          1..4860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2135..2514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2515..2718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2669..2689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2763..2782
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2763..2781
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4440..4574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4477..4496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4531..4550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4695..4860
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgatcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     2161 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     2221 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2281 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2341 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2401 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2461 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2521 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2581 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2641 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2701 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
     2761 attaatacga ctcactatag ggagacccaa gctggctagt ggatcccggt ccgaagcgcg
     2821 cggaattcat ggaagccaca ccgaccccgg ccgatctgtt cagcgaagat tacctggtgg
     2881 ataccctgga tggcctgacc gtggatgatc agcaggccgt tctggcaagc ctgagcttca
     2941 gcaagttcct gaagcatgcc aaagtgcgtg actggtgtgc ccaggcaaaa atccagccta
     3001 gtatgccggc cctgcgcatg gcatacaatt acttcctgtt cagtaaagtt ggcgaattta
     3061 ttggtagcga agacgtttgt aattttttcg tggatcgtgt gtttggcggc gtgcgcctgc
     3121 tggatgttgc aagcgtttat gccgcctgta gtcagatgaa tgcccatcag cgccatcaca
     3181 tctgttgtct ggtggaacgt gcaaccagca gtcaaagcct gaacccggtg tgggatgcct
     3241 tacgcgatgg tatcatcagc agcagcaagt tccattgggc cgtgaagcag cagaacacca
     3301 gcaagaaaat tttcagcccg tggccgatca caaacaacca ttttgtggcc ggcccgttag
     3361 cctttggtct gcgttgcgaa gaggtggtta aaaccctgct ggcaacactg ctgcatcctg
     3421 atgaggccaa ctgcctggac tacggtttta tgcagagccc gcagaacggt atctttggcg
     3481 ttagcctgaa ttttgccgcc aatgtgaaaa ccgacaccga aggccgtctg cagttcgatc
     3541 ctaactgtaa ggtgtatcag attaaatgcc gctttaaata tacattcgca aaaatggaat
     3601 gtgatccgat ctatgccgcc taccagcgct tatatgaagc accgggcaaa ctggcactga
     3661 aggacttttt ctacagcatt agcaagccgg ccgttgagta cgttggtctg ggcaaattac
     3721 cgagcgaaag cgactatctg gtggcctatg atcaggaatg ggaagcctgc ccgcgcaaaa
     3781 aacgcaaact gaccccgctg cacaacctga ttcgtgagtg tatcctgcac aacagcacca
     3841 ccgagagcga cgtttacgtt ctgaccgatc cgcaggatac ccgtggccag atcagtatca
     3901 aggcacgctt caaggccaac ctgtttgtga acgtgcgcca cagctacttt taccaggtgc
     3961 tgctgcagag tagtatcgtg gaggagtata ttggcctgga tagcggtatt ccgcgtctgg
     4021 gtagcccgaa atattacatc gccaccggtt tcttccgtaa acgcggctat caggatccgg
     4081 tgaattgcac aattggtggc gacgccctgg atcctcatgt ggagattccg accctgctga
     4141 ttgtgacccc ggtgtatttc cctcgcggcg ccaagcaccg tctgctgcat caggcagcca
     4201 atttctggag ccgcagcgcc aaagatacat ttccgtatat caaatgggat tttagctacc
     4261 tgagcgccaa cgtgccgcac agcccgtaat ctagagcctg cagtctcgac aagcttgtcg
     4321 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4381 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4441 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4501 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4561 atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga
     4621 aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat
     4681 acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     4741 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     4801 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
//
