LOCUS       sequence-381559-        8430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2442..2466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2957..2977
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2984..3013
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3023..3463
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3506..3526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3677..3910
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3914..3940
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3944..3970
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3983..4001)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3984..4001)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4021..4038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4027..4251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4297..4725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4384..4403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4594..4615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4734..4754)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4739..5068
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4919..5054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4981..5000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5135..5929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5189..5208)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5799..5818
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6103..6224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6140..6159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6194..6213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6273..6289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6273..6289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6286..6308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6297..6313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6321..6327,6328..6345,6346..6351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6366..6387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6504..6521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6675..7263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6755..6774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7434..8225,8226..8294))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8057..8076
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8295..8399)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctccaccat gaagacgatc atcgccctga gctacatctt
      961 ctgcctggta ttcgccgact acaaggacga tgatgacgcc agcatcgata tgcccccctg
     1021 ccagccacag cgacccctgc tgctgttgct gctgctgctg gcctgccagc cacaggtccc
     1081 ctccgctcag gtgatggact tcctgtttga gaagtggaag ctctacggtg accagtgtca
     1141 ccacaacctg agcctgctgc cccctcccac ggagctggtg tgcaacagaa ccttcgacaa
     1201 gtattcctgc tggccggaca cccccgccaa taccacggcc aacatctcct gcccctggta
     1261 cctgccttgg caccacaaag tgcaacaccg cttcgtgttc aagagatgcg ggcccgacgg
     1321 tcagtgggtg cgtggacccc gggggcagcc ttggcgtgat gcctcccagt gccagatgga
     1381 tggcgaggag attgaggtcc agaaggaggt ggccaagatg tacagcagct tccaggtgat
     1441 gtacacagtg ggctacagcc tgtccctggg ggccctgctc ctcgccttgg ccatcctggg
     1501 gggcctcagc aagctgcact gcacccgcaa tgccatccac gcgaatctgt ttgcgtcctt
     1561 cgtgctgaaa gccagctccg tgctggtcat tgatgggctg ctcaggaccc gctacagcca
     1621 gaaaattggc gacgacctca gtgtcagcac ctggctcagt gatggagcgg tggctggctg
     1681 ccgtgtggcc gcggtgttca tgcaatatgg catcgtggcc aactactgct ggctgctggt
     1741 ggagggcctg tacctgcaca acctgctggg cctggccacc ctccccgaga ggagcttctt
     1801 cagcctctac ctgggcatcg gctggggtgc ccccatgctg ttcgtcgtcc cctgggcagt
     1861 ggtcaagtgt ctgttcgaga acgtccagtg ctggaccagc aatgacaaca tgggcttctg
     1921 gtggatcctg cggttccccg tcttcctggc catcctgatc aacttcttca tcttcgtccg
     1981 catcgttcag ctgctcgtgg ccaagctgcg ggcacggcag atgcaccaca cagactacaa
     2041 gttccggctg gccaagtcca cgctgaccct catccctctg ctgggcgtcc acgaagtggt
     2101 cttcgccttc gtgacggacg agcacgccca gggcaccctg cgctccgcca agctcttctt
     2161 cgacctcttc ctcagctcct tccagggcct gctggtggct gtcctctact gcttcctcaa
     2221 caaggaggtg cagtcggagc tgcggcggcg ttggcaccgc tggcgcctgg gcaaagtgct
     2281 atgggaggag cggaacacca gcaaccacag ggcctcatct tcgcccggcc acggccctcc
     2341 cagcaaggag ctgcagtttg ggaggggtgg tggcagccag gattcatctg cggagacccc
     2401 cttggctggt ggcctcccta gattggctga gagccccttc gacccagctt tcttgtacaa
     2461 agtggttgat ggtaccgccc ggggacgcac cccacccagc ctgggtcccc aagatgagtc
     2521 ctgcaccacc gccagctcct ccctggccaa ggacacttca tcggatgtcg gaggtggagg
     2581 tagcggaggt ggaggtagcg gagagagctt gtttaaggga ccacgtgatt acaacccgat
     2641 atcgagcacc atttgtcatt tgacgaatga atctgatggg cacacaacat cgttgtatgg
     2701 tattggattt ggtcccttca tcattacaaa caagcacttg tttcgtcgca ataatggaac
     2761 actgttggtc caatcactac atggtgtatt caaggtcaag gacaccacga ctttgcaaca
     2821 acacctcatt gatgggaggg acatgatgat tattcgcatg cctaaggatt tcccaccatt
     2881 tcctcaaaag ctgaaattta gagagccaca aagggaagag cgcatatgtc ttgtgacaac
     2941 caacttccaa gctcccgaga acctgtactt ccagggttct agagaacaga agctgatctc
     3001 agaagaggat ctggcccggg ccatgaagct actgtcttct atcgaacaag catgcgatat
     3061 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa
     3121 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca
     3181 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc
     3241 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt
     3301 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc
     3361 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc
     3421 ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat tcccggggat
     3481 cagcttgata attcgattca ccatgcccaa gaagaagagg aaggtggcgt acagccgcgc
     3541 gcgtacgaaa aacaattacg ggtctaccat cgagggcctg ctcgatctcc cggacgacga
     3601 cgcccccgaa gaggcggggc tggcggctcc gcgcctgtcc tttctccccg cgggacacac
     3661 gcgcagactg tcgacggccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga
     3721 cggcgaggac gtcgcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt
     3781 gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc
     3841 tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga
     3901 gtacggtggg ggctacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     3961 ggactatgca tgagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     4021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5101 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5281 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5581 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5641 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5821 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5941 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7201 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7261 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7321 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7381 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7441 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7501 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7561 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7621 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7681 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7741 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7801 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7861 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7921 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7981 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8041 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8101 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8161 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8221 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8281 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8341 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8401 acatttcccc gaaaagtgcc acctgacgtc
//
