LOCUS       sequence-381560-        3978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed Renilla Luciferase with SOX resistance element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Hartenian E, Mendez AS, Didychuk AL, Khosla S, Glaunsinger BA
  TITLE     DNA processing by the Kaposi's sarcoma-associated herpesvirus 
            alkaline exonuclease SOX contributes to viral gene expression and 
            infectious virion production.
  JOURNAL   Nucleic Acids Res. 2023 Jan 11;51(1):182-197. doi: 
            10.1093/nar/gkac1190.
  PUBMED    36537232
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-11- 11"; volume: "51"; issue: 
            "1"; pages: "182-197"
COMMENT     Sequence Label: pMDC Renilla Luc SRE
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..53
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      106..462
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(857..1516)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1431..1450
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1517..1619)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     polyA_signal    1791..1925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1815..1834)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1869..1888
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2291..3226)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     complement(3289..3308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3290..3308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3353..3556)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3382..3402)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3557..3936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aaacccatgt gcctggcaga taacttcgta taatgtatgc tatacgaagt tatggtaccg
       61 cggccgcgta gaggatctgt tgatcagcag ttcaacctgt tgatagtacg tactaagctc
      121 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
      181 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
      241 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
      301 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
      361 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
      421 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt agcttcacgc
      481 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
      541 ccgcagaaac ggtgctgacc ccggatgaat gtcagctggg aggcagaata aatgatcata
      601 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
      661 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      721 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
      781 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
      841 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      901 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      961 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1021 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1081 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1141 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1201 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1261 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     1321 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1381 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1441 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1501 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1561 cgcccggtag tgatcttatt tcattatggt gaaagttgga ccctcttacg tgccgatcaa
     1621 cgtctcattt tcgccaaaag ttggcccaga tctatgtcgg gtgcggagaa agaggtaatg
     1681 aaatggcacc taggggttat gatagttatt gctcagcggt ggcagcagcc aactcagctt
     1741 cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca gatccagaca
     1801 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1861 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1921 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1981 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagtac
     2041 ttctcgacaa gcttgtcgag actgcaggct ctagacttaa aaatataaat atgacttaca
     2101 taaattaact cagcttcaca tatttaaata ttaataaatt aaaaataatt aaaatagtgt
     2161 cctaacgctc atacttttag ttctccatag agaacaacat aagttctgtg cccagtggac
     2221 aggtttctga ccagaagaag gaatgcccat taacaacaac aatctgaggt gcccatgatc
     2281 tgcagaattc ttattgttca tttttgagaa ctcgctcaac gaacgatttg atatattttc
     2341 ccatttcatc aggtgcatct tcttgcgaaa aatgaagacc ttttactttg acaaattcag
     2401 tattaggaaa cttcttggca ccttcaacaa tagcattgga aaagaatcct gggtccgatt
     2461 caataaacat ttttggtaaa tcatcacttg cacgtagata agcattataa ttcctaacaa
     2521 tttgtacaac gtcaggttta ccacctttta ctaacgggat ttcacgaggc catgataatg
     2581 ttggacgacg aacttcacct ttctctttga atggttcaag atatgctgca aattcttctg
     2641 gttctaactt tctcatgatt tttgatggca acatggtttc cacgaagaag ttattctcca
     2701 aaaccatttt ttctccttct tcagatttga tcaacgcaat atcttcttca atatcaggcc
     2761 attcatccca tgattcaatc acatctacta cactttcagc gtgaactatt gctttgatct
     2821 tatcttgatg ctcatagcta taatgaaatg ccaaacaagc accccaatca tggccgacaa
     2881 aaatgatctt ctttggtaaa ttaagaagtt caaaccatgc agtaagatat ttgtaatgat
     2941 caagtaacct ataagaacca ttaccagatt tgcctgattt gcccatacca ataaggtctg
     3001 gtataataca ccgcgctact ggctcaatat gtggcacaac atgtcgccat aaataagaag
     3061 aggccgcgtt accatgtaaa aaaataacag cattttctgc atgtttttct gaatcataat
     3121 aattaataaa tgaatcaaga acattcattt gtttacatct ggcccaccac tgcggaccag
     3181 ttatcatccg tttcctttgt tctggatcat aaactttcga agtcatgtcg acgtaggcct
     3241 ttgaattccg cgcgcttcgg accgggatcc actagccagc ttgggtctcc ctatagtgag
     3301 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
     3361 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     3421 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     3481 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     3541 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     3601 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     3661 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     3721 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     3781 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     3841 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     3901 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     3961 cgcgaatcga tactagta
//
