LOCUS       sequence-381561-        3090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of anti-CRISPR protein AcrVIB1 in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur
            A, Backofen R, Beisel CL
  TITLE     Anti-CRISPR prediction using deep learning reveals an inhibitor of 
            Cas13b nucleases.
  JOURNAL   Mol Cell. 2022 May 24. pii: S1097-2765(22)00437-3. doi: 
            10.1016/j.molcel.2022.05.003.
  PUBMED    35649413
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 24. pii: S1097-2765(22)00437-3. doi: 
            10.1016/j.molcel.2022.05.003."
COMMENT     Sequence Label: pAcrVIB1-cloDF-amp
FEATURES             Location/Qualifiers
     source          1..3090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(766..800)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(823..1614,1615..1683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1446..1465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1684..1788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1805..1823
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1847..1881
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             1923..1945
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      2306..2392
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(join(2412..3090,1..60))
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
       61 actagtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
      121 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
      181 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
      241 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
      301 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
      361 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
      421 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
      481 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
      541 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
      601 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
      661 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
      721 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
      781 cggcaaccga gcgttctgaa caaatccaga tggagttctg agttaccaat gcttaatcag
      841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgga cattaaccta
     1801 taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagttcttga cagctagctc
     1861 agtcctaggt ataatgctag cctctaggat ccgaattcga gctcaggagg gctagcaata
     1921 attttgttta actttaagaa ggagatatac catgaaggac ctggacctgt ccaagcttaa
     1981 gggtgaagag attgcccagt ggcttttgaa caacaagaaa gccactgcaa tccaactctc
     2041 atccgaacgt acggatacgg acgatgggtt catgcacatt ctggtacaca aggatgagta
     2101 cgtggaaatc atttacagct acttgaagat tgatgaggac gatgtaatgc aaaacttcac
     2161 gatctactcg aaacgctggg gtaacatcga taatagctac tttgagttgc aaacctttga
     2221 aggggaaatc ttcacgggtg aaagcgacaa gatcctttgt ggagtgttgt cattaggcga
     2281 cttaactaca ctcaagtaaa ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2341 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     2401 ctagacctag ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg
     2461 ggactaacat gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc ataggctccg
     2521 ccctcctgcc agagttcaca taaacagacg cttttccggt gcatctgtgg gagccgtgag
     2581 gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt
     2641 ttccggcggg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc
     2701 tgttccgcct ttctccctta cgggaagtgt ggcgctttct catagctcac acactggtat
     2761 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
     2821 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacggt
     2881 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
     2941 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     3001 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     3061 tcgatcaaac cacctcccca ggtggttttt
//
