LOCUS       sequence-381565-        8328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2340..2364)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2855..2875
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2882..2911
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2921..3361
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3404..3424
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3575..3808
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3812..3838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3842..3868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3881..3899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3882..3899)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3919..3936)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3925..4149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4195..4623
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4282..4301)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4492..4513
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4632..4652)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4637..4966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4817..4952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4879..4898
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5033..5827
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5087..5106)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5697..5716
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6001..6122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6038..6057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6092..6111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6171..6187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6171..6187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6184..6206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6195..6211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6219..6225,6226..6243,6244..6249))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6264..6285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6402..6419)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6573..7161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6653..6672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7332..8123,8124..8192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7955..7974
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8193..8297)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag aagggccgtc aaggcccacc atggccggcg cccccggccc
      961 gctgcgcctt gcgctgctgc tgctcgggat ggtgggcagg gccggccccc gcccccaggg
     1021 tgccactgtg tccctctggg agacggtgca gaaatggcga gaataccgac gccagtgcca
     1081 gcgctccctg actgaggatc cacctcctgc cacagacttg ttctgcaacc ggaccttcga
     1141 tgaatacgcc tgctggccag atggggagcc aggctcgttc gtgaatgtca gctgcccctg
     1201 gtacctgccc tgggccagca gtgtgccgca gggccacgtg taccggttct gcacagctga
     1261 aggcctctgg ctgcagaagg acaactccag cctgccctgg agggacttgt cggagtgcga
     1321 ggagtccaag cgaggggaaa gaagctcccc ggaggagcag ctcctgttcc tctacatcat
     1381 ctacacggtg ggctacgcac tctccttctc tgctctggtt atcgcctctg cgatcctcct
     1441 cggcttcaga cacctgcact gcaccaggaa ctacatccac ctgaacctgt ttgcatcctt
     1501 catcctgcga gcattgtccg tcttcatcaa ggacgcagcc ctgaagtgga tgtatagcac
     1561 agccgcccag cagcaccagt gggatgggct cctctcctac caggactctc tgagctgccg
     1621 cctggtgttt ctgctcatgc agtactgtgt ggcggccaat tactactggc tcttggtgga
     1681 gggcgtgtac ctgtacacac tgctggcctt ctcggtctta tctgagcaat ggatcttcag
     1741 gctctacgtg agcataggct ggggtgttcc cctgctgttt gttgtcccct ggggcattgt
     1801 caagtacctc tatgaggacg agggctgctg gaccaggaac tccaacatga actactggct
     1861 cattatccgg ctgcccattc tctttgccat tggggtgaac ttcctcatct ttgttcgggt
     1921 catctgcatc gtggtatcca aactgaaggc caatctcatg tgcaagacag acatcaaatg
     1981 cagacttgcc aagtccacgc tgacactcat ccccctgctg gggactcatg aggtcatctt
     2041 tgcctttgtg atggacgagc acgcccgggg gaccctgcgc ttcatcaagc tgtttacaga
     2101 gctctccttc acctccttcc aggggctgat ggtggccata ttatactgct ttgtcaacaa
     2161 tgaggtccag ctggaatttc ggaagagctg ggagcgctgg cggcttgagc acttgcacat
     2221 ccagagggac agcagcatga agcccctcaa gtgtcccacc agcagcctga gcagtggagc
     2281 cacggcgggc agcagcatgt acacagccac ttgccaggcc tcctgcagcg gcctcatggg
     2341 cccagctttc ttgtacaaag tggttgatgg taccgcccgg ggacgcaccc cacccagcct
     2401 gggtccccaa gatgagtcct gcaccaccgc cagctcctcc ctggccaagg acacttcatc
     2461 ggatgtcgga ggtggaggta gcggaggtgg aggtagcgga gagagcttgt ttaagggacc
     2521 acgtgattac aacccgatat cgagcaccat ttgtcatttg acgaatgaat ctgatgggca
     2581 cacaacatcg ttgtatggta ttggatttgg tcccttcatc attacaaaca agcacttgtt
     2641 tcgtcgcaat aatggaacac tgttggtcca atcactacat ggtgtattca aggtcaagga
     2701 caccacgact ttgcaacaac acctcattga tgggagggac atgatgatta ttcgcatgcc
     2761 taaggatttc ccaccatttc ctcaaaagct gaaatttaga gagccacaaa gggaagagcg
     2821 catatgtctt gtgacaacca acttccaagc tcccgagaac ctgtacttcc agggttctag
     2881 agaacagaag ctgatctcag aagaggatct ggcccgggcc atgaagctac tgtcttctat
     2941 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     3001 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     3061 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     3121 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     3181 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     3241 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     3301 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     3361 gccggaattc ccggggatca gcttgataat tcgattcacc atgcccaaga agaagaggaa
     3421 ggtggcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg agggcctgct
     3481 cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc gcctgtcctt
     3541 tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg tcagcctggg
     3601 ggacgagctc cacttagacg gcgaggacgt cgcgatggcg catgccgacg cgctagacga
     3661 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga
     3721 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga
     3781 tgcccttgga attgacgagt acggtggggg ctacccatac gatgttcctg actatgcggg
     3841 ctatccctat gacgtcccgg actatgcatg agggccctat tctatagtgt cacctaaatg
     3901 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3961 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4021 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4081 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4141 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4201 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4261 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4321 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4381 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4441 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4501 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4561 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4621 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4801 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4861 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4921 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4981 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5041 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5101 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5161 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5221 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5281 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5341 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5401 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5461 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5521 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5581 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5641 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5701 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5761 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5821 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5881 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5941 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6121 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6181 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6241 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6301 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6361 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6421 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6481 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6541 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6601 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6661 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6721 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6781 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6841 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6901 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6961 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7021 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7081 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7141 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7201 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7261 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7321 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7381 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7441 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7501 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7561 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7621 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7681 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7741 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7801 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7861 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7921 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7981 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8041 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8101 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8161 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8221 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8281 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
