LOCUS       sequence-381566-        8274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_GCGR-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2382..2406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2801..2821
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2828..2857
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2867..3307
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3350..3370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3521..3754
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3758..3784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3788..3814
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3827..3845)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3828..3845)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3865..3882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3871..4095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4141..4569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4228..4247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4438..4459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4578..4598)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4583..4912
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4763..4898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4825..4844
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4979..5773
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5033..5052)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5643..5662
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5947..6068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5984..6003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6038..6057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6117..6133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6117..6133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6130..6152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6141..6157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6165..6171,6172..6189,6190..6195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6210..6231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6348..6365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6519..7107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6599..6618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7278..8069,8070..8138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7901..7920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8139..8243)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgcccccctg
      961 ccagccacag cgacccctgc tgctgttgct gctgctgctg gcctgccagc cacaggtccc
     1021 ctccgctcag gtgatggact tcctgtttga gaagtggaag ctctacggtg accagtgtca
     1081 ccacaacctg agcctgctgc cccctcccac ggagctggtg tgcaacagaa ccttcgacaa
     1141 gtattcctgc tggccggaca cccccgccaa taccacggcc aacatctcct gcccctggta
     1201 cctgccttgg caccacaaag tgcaacaccg cttcgtgttc aagagatgcg ggcccgacgg
     1261 tcagtgggtg cgtggacccc gggggcagcc ttggcgtgat gcctcccagt gccagatgga
     1321 tggcgaggag attgaggtcc agaaggaggt ggccaagatg tacagcagct tccaggtgat
     1381 gtacacagtg ggctacagcc tgtccctggg ggccctgctc ctcgccttgg ccatcctggg
     1441 gggcctcagc aagctgcact gcacccgcaa tgccatccac gcgaatctgt ttgcgtcctt
     1501 cgtgctgaaa gccagctccg tgctggtcat tgatgggctg ctcaggaccc gctacagcca
     1561 gaaaattggc gacgacctca gtgtcagcac ctggctcagt gatggagcgg tggctggctg
     1621 ccgtgtggcc gcggtgttca tgcaatatgg catcgtggcc aactactgct ggctgctggt
     1681 ggagggcctg tacctgcaca acctgctggg cctggccacc ctccccgaga ggagcttctt
     1741 cagcctctac ctgggcatcg gctggggtgc ccccatgctg ttcgtcgtcc cctgggcagt
     1801 ggtcaagtgt ctgttcgaga acgtccagtg ctggaccagc aatgacaaca tgggcttctg
     1861 gtggatcctg cggttccccg tcttcctggc catcctgatc aacttcttca tcttcgtccg
     1921 catcgttcag ctgctcgtgg ccaagctgcg ggcacggcag atgcaccaca cagactacaa
     1981 gttccggctg gccaagtcca cgctgaccct catccctctg ctgggcgtcc acgaagtggt
     2041 cttcgccttc gtgacggacg agcacgccca gggcaccctg cgctccgcca agctcttctt
     2101 cgacctcttc ctcagctcct tccagggcct gctggtggct gtcctctact gcttcctcaa
     2161 caaggaggtg cagtcggagc tgcggcggcg ttggcaccgc tggcgcctgg gcaaagtgct
     2221 atgggaggag cggaacacca gcaaccacag ggcctcatct tcgcccggcc acggccctcc
     2281 cagcaaggag ctgcagtttg ggaggggtgg tggcagccag gattcatctg cggagacccc
     2341 cttggctggt ggcctcccta gattggctga gagccccttc gacccagctt tcttgtacaa
     2401 agtggttgat gtcggaggtg gaggtagcgg aggtggaggt agcggagaga gcttgtttaa
     2461 gggaccacgt gattacaacc cgatatcgag caccatttgt catttgacga atgaatctga
     2521 tgggcacaca acatcgttgt atggtattgg atttggtccc ttcatcatta caaacaagca
     2581 cttgtttcgt cgcaataatg gaacactgtt ggtccaatca ctacatggtg tattcaaggt
     2641 caaggacacc acgactttgc aacaacacct cattgatggg agggacatga tgattattcg
     2701 catgcctaag gatttcccac catttcctca aaagctgaaa tttagagagc cacaaaggga
     2761 agagcgcata tgtcttgtga caaccaactt ccaagctccc gagaacctgt acttccaggg
     2821 ttctagagaa cagaagctga tctcagaaga ggatctggcc cgggccatga agctactgtc
     2881 ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa
     2941 accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa
     3001 aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga
     3061 acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc
     3121 tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga
     3181 tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca
     3241 tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac
     3301 tgtatcgccg gaattcccgg ggatcagctt gataattcga ttcaccatgc ccaagaagaa
     3361 gaggaaggtg gcgtacagcc gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg
     3421 cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct
     3481 gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag
     3541 cctgggggac gagctccact tagacggcga ggacgtcgcg atggcgcatg ccgacgcgct
     3601 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
     3661 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
     3721 taccgatgcc cttggaattg acgagtacgg tgggggctac ccatacgatg ttcctgacta
     3781 tgcgggctat ccctatgacg tcccggacta tgcatgaggg ccctattcta tagtgtcacc
     3841 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4561 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4681 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4741 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4861 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4921 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     4981 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5041 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5101 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5161 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5221 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5281 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5341 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5401 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5461 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5521 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5581 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5641 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5701 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5761 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5821 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5881 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5941 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6061 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6121 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6181 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6241 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6301 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7261 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7321 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7381 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7441 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7501 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7561 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7621 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7681 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7741 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7801 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7861 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7921 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7981 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8041 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8101 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8161 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8221 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
