LOCUS       sequence-381569-        8037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2145..2169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2564..2584
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2591..2620
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2630..3070
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3113..3133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3284..3517
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3521..3547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3551..3577
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3590..3608)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3591..3608)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3628..3645)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3634..3858
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3904..4332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3991..4010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4201..4222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4341..4361)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4346..4675
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4526..4661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4588..4607
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4742..5536
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4796..4815)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5406..5425
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5710..5831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5747..5766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5801..5820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5880..5896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5880..5896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5893..5915)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5904..5920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5928..5934,5935..5952,5953..5958))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5973..5994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6111..6128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6282..6870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6362..6381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7041..7832,7833..7901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7664..7683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7902..8006)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atgctcatgg cgtccaccac ttccgctgtg cctgggcatc cctctctgcc
     1081 cagcctgccc agcaacagca gccaggagag gccactggac acccgggacc cgctgctagc
     1141 ccgggcagag ctggcgctgc tctccatagt ctttgtggct gtggccctga gcaatggcct
     1201 ggtgctggcg gccctagctc ggcggggccg gcggggccac tgggcaccca tacacgtctt
     1261 cattggccac ttgtgcctgg ccgacctggc cgtggctctg ttccaagtgc tgccccagct
     1321 ggcctggaag gccaccgacc gcttccgtgg gccagatgcc ctgtgtcggg ccgtgaagta
     1381 tctgcagatg gtgggcatgt atgcctcctc ctacatgatc ctggccatga cgctggaccg
     1441 ccaccgtgcc atctgccgtc ccatgctggc gtaccggcat ggaagtgggg ctcactggaa
     1501 ccggccggtg ctagtggctt gggccttctc gctccttctc agcctgcccc agctcttcat
     1561 cttcgcccag cgcaacgtgg aaggtggcag cggggtcact gactgctggg cctgctttgc
     1621 ggagccctgg ggccgtcgca cctatgtcac ctggattgcc ctgatggtgt tcgtggcacc
     1681 taccctgggt atcgccgcct gccaggtgct catcttccgg gagattcatg ccagtctggt
     1741 gccagggcca tcagagaggc ctggggggcg ccgcagggga cgccggacag gcagccccgg
     1801 tgagggagcc cacgtgtcag cagctgtggc caagactgtg aggatgacgc tagtgattgt
     1861 ggtcgtctat gtgctgtgct gggcaccctt cttcctggtg cagctgtggg ccgcgtggga
     1921 cccggaggca cctctggaag gggcgccctt tgtgctactc atgttgctgg ccagcctcaa
     1981 cagctgcacc aacccctgga tctatgcatc tttcagcagc agcgtgtcct cagagctgcg
     2041 aagcttgctc tgctgtgccc ggggacgcac cccacccagc ctgggtcccc aagatgagtc
     2101 ctgcaccacc gccagctcct ccctggccaa ggacacttca tcggacccag ctttcttgta
     2161 caaagtggtt gatgtcggag gtggaggtag cggaggtgga ggtagcggag agagcttgtt
     2221 taagggacca cgtgattaca acccgatatc gagcaccatt tgtcatttga cgaatgaatc
     2281 tgatgggcac acaacatcgt tgtatggtat tggatttggt cccttcatca ttacaaacaa
     2341 gcacttgttt cgtcgcaata atggaacact gttggtccaa tcactacatg gtgtattcaa
     2401 ggtcaaggac accacgactt tgcaacaaca cctcattgat gggagggaca tgatgattat
     2461 tcgcatgcct aaggatttcc caccatttcc tcaaaagctg aaatttagag agccacaaag
     2521 ggaagagcgc atatgtcttg tgacaaccaa cttccaagct cccgagaacc tgtacttcca
     2581 gggttctaga gaacagaagc tgatctcaga agaggatctg gcccgggcca tgaagctact
     2641 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
     2701 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
     2761 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
     2821 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga
     2881 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa
     2941 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca
     3001 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt
     3061 gactgtatcg ccggaattcc cggggatcag cttgataatt cgattcacca tgcccaagaa
     3121 gaagaggaag gtggcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga
     3181 gggcctgctc gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg
     3241 cctgtccttt ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt
     3301 cagcctgggg gacgagctcc acttagacgg cgaggacgtc gcgatggcgc atgccgacgc
     3361 gctagacgat ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac
     3421 cccccacgac tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat
     3481 gtttaccgat gcccttggaa ttgacgagta cggtgggggc tacccatacg atgttcctga
     3541 ctatgcgggc tatccctatg acgtcccgga ctatgcatga gggccctatt ctatagtgtc
     3601 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3661 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3721 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3781 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3841 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     3901 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3961 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4021 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4081 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4201 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4261 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4321 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4501 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4681 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4741 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4801 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4861 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4921 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4981 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5041 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5101 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5161 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5221 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5281 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5341 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5401 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5461 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5521 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5581 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5641 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5701 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5761 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5821 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
