LOCUS       sequence-381570-        8253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_GLP1R-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2361..2385)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2780..2800
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2807..2836
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2846..3286
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3329..3349
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3500..3733
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3737..3763
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3767..3793
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3806..3824)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3807..3824)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3844..3861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3850..4074
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4120..4548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4207..4226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4417..4438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4557..4577)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4562..4891
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4742..4877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4804..4823
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4958..5752
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5012..5031)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5622..5641
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5926..6047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5963..5982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6017..6036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6096..6112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6096..6112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6109..6131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6120..6136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6144..6150,6151..6168,6169..6174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6189..6210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6327..6344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6498..7086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6578..6597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7257..8048,8049..8117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7880..7899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8118..8222)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca gaagggccgt caaggcccac
      961 catggccggc gcccccggcc cgctgcgcct tgcgctgctg ctgctcggga tggtgggcag
     1021 ggccggcccc cgcccccagg gtgccactgt gtccctctgg gagacggtgc agaaatggcg
     1081 agaataccga cgccagtgcc agcgctccct gactgaggat ccacctcctg ccacagactt
     1141 gttctgcaac cggaccttcg atgaatacgc ctgctggcca gatggggagc caggctcgtt
     1201 cgtgaatgtc agctgcccct ggtacctgcc ctgggccagc agtgtgccgc agggccacgt
     1261 gtaccggttc tgcacagctg aaggcctctg gctgcagaag gacaactcca gcctgccctg
     1321 gagggacttg tcggagtgcg aggagtccaa gcgaggggaa agaagctccc cggaggagca
     1381 gctcctgttc ctctacatca tctacacggt gggctacgca ctctccttct ctgctctggt
     1441 tatcgcctct gcgatcctcc tcggcttcag acacctgcac tgcaccagga actacatcca
     1501 cctgaacctg tttgcatcct tcatcctgcg agcattgtcc gtcttcatca aggacgcagc
     1561 cctgaagtgg atgtatagca cagccgccca gcagcaccag tgggatgggc tcctctccta
     1621 ccaggactct ctgagctgcc gcctggtgtt tctgctcatg cagtactgtg tggcggccaa
     1681 ttactactgg ctcttggtgg agggcgtgta cctgtacaca ctgctggcct tctcggtctt
     1741 atctgagcaa tggatcttca ggctctacgt gagcataggc tggggtgttc ccctgctgtt
     1801 tgttgtcccc tggggcattg tcaagtacct ctatgaggac gagggctgct ggaccaggaa
     1861 ctccaacatg aactactggc tcattatccg gctgcccatt ctctttgcca ttggggtgaa
     1921 cttcctcatc tttgttcggg tcatctgcat cgtggtatcc aaactgaagg ccaatctcat
     1981 gtgcaagaca gacatcaaat gcagacttgc caagtccacg ctgacactca tccccctgct
     2041 ggggactcat gaggtcatct ttgcctttgt gatggacgag cacgcccggg ggaccctgcg
     2101 cttcatcaag ctgtttacag agctctcctt cacctccttc caggggctga tggtggccat
     2161 attatactgc tttgtcaaca atgaggtcca gctggaattt cggaagagct gggagcgctg
     2221 gcggcttgag cacttgcaca tccagaggga cagcagcatg aagcccctca agtgtcccac
     2281 cagcagcctg agcagtggag ccacggcggg cagcagcatg tacacagcca cttgccaggc
     2341 ctcctgcagc ggcctcatgg gcccagcttt cttgtacaaa gtggttgatg tcggaggtgg
     2401 aggtagcgga ggtggaggta gcggagagag cttgtttaag ggaccacgtg attacaaccc
     2461 gatatcgagc accatttgtc atttgacgaa tgaatctgat gggcacacaa catcgttgta
     2521 tggtattgga tttggtccct tcatcattac aaacaagcac ttgtttcgtc gcaataatgg
     2581 aacactgttg gtccaatcac tacatggtgt attcaaggtc aaggacacca cgactttgca
     2641 acaacacctc attgatggga gggacatgat gattattcgc atgcctaagg atttcccacc
     2701 atttcctcaa aagctgaaat ttagagagcc acaaagggaa gagcgcatat gtcttgtgac
     2761 aaccaacttc caagctcccg agaacctgta cttccagggt tctagagaac agaagctgat
     2821 ctcagaagag gatctggccc gggccatgaa gctactgtct tctatcgaac aagcatgcga
     2881 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
     2941 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
     3001 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
     3061 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
     3121 gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc
     3181 ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc
     3241 atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg aattcccggg
     3301 gatcagcttg ataattcgat tcaccatgcc caagaagaag aggaaggtgg cgtacagccg
     3361 cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga
     3421 cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca
     3481 cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     3541 agacggcgag gacgtcgcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     3601 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     3661 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     3721 cgagtacggt gggggctacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     3781 cccggactat gcatgagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     3841 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3901 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3961 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4021 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4081 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4561 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4621 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4681 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4741 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4801 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4861 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4921 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4981 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5041 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5101 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5161 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5221 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5281 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5341 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5401 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5461 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5521 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5581 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5641 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5701 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5761 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5821 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5881 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6061 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6121 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6181 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6241 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6301 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6901 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7141 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7201 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7261 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7321 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7381 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7441 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7501 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7561 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7621 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7681 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7741 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7801 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7861 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7921 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7981 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8041 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8101 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8221 cgcacatttc cccgaaaagt gccacctgac gtc
//
