LOCUS       sequence-381571-        7135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector, high-level expression of somatodendritic-targeted EGFP 
            (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the
            presence of Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Sohn J, Takahashi M, Okamoto S, Ishida Y, Furuta T, Hioki H
  TITLE     A Single Vector Platform for High-Level Gene Transduction of Central
            Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off 
            System.
  JOURNAL   PLoS One. 2017 Jan 6;12(1):e0169611. doi: 
            10.1371/journal.pone.0169611. eCollection 2017.
  PUBMED    28060929
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-01-6"; volume: "12(1):e0169611. doi"; pages: " 
            10.1371/journal.pone.0169611. eCollection 2017"
COMMENT     Sequence Label: pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]
FEATURES             Location/Qualifiers
     source          1..7135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..137
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    152..176
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    276..397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(313..332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     367..386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(421..429,430..465,466..468,469..504,
                     505..507,508..543,544..549,550..1167))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        1688..2002
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1696..1714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1732..1750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1767..1785
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1803..1821
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1839..1857
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1874..1892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1910..1928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1975..1999
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2036..2069
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2132..2165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2180..2857)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(2180..2857)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2242..2265)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2440..2459)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2709..2727
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(2933..2998)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(join(3152..3862,3863..3865))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(3152..3862,3863..3865))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3194..3215)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3543..3562
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3802..3823
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3922..3931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3950..3983)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4054..4087
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4111..4335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4358..4382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   4405..4545
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4405..4534
                     /label=AAV2 ITR
     rep_origin      4620..5075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4707..4726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4917..4938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5092..5111)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5211..5233
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5271..5289)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5357..5461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5462..5530,5531..6322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5680..5699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6493..7081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6982..7001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
       61 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      121 catcactagg ggttcctgcg gccgcacgcg tacaagtttg tacaaaaaag caggctttgg
      181 atcttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgagat ccttacttag
      421 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
      481 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
      541 ggccggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
      601 gccgaaaagg aaggcaggtt cggctccctg atggtcgaac agctcaattg cttgtctcag
      661 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
      721 ctgatcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
      781 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
      841 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
      901 agcacagcgg aatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
      961 ggggcagaag tgggtatgat gcctgtccag catctcgatt gccagggcat cgagcagggc
     1021 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     1081 tttccttgtc gtcaggcctt cgataccgac ttcattgagt aattccagag cagagtttat
     1141 gactttgctc ttgtccagtc tagacatggt gaattcgata tcaagcttat cgataccgtc
     1201 cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc gcgcctcgcg tggtgcggtc
     1261 cggctgggcc ggcggcggcg cggacgcgac caaggtggcc gggaagggga gtttgcgggg
     1321 gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg gcggtggcgc gcgccgccag
     1381 gcgggggcga aggcactgtc cgcggtgctg aagctggcag tgcgcacgcg cctcgccgca
     1441 tcctgtttcc cctccccctc tctgataggg gatgcgcaat ttggggaatg ggggttgggt
     1501 gcttgtccag tgggtcgggg tcggtcgtca ggtaggcacc cccaccccgc ctcatcctgg
     1561 tcctaaaacc cacttgcact catacgcagg gccctctgca gctcgaccgc gccgccccgg
     1621 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     1681 ggggctcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     1741 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     1801 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     1861 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     1921 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
     1981 tttagtgaac cgtcagatcg cctggagaat tcgagctcgg tacccgggga tccccataac
     2041 ttcgtataaa gtatcctata cgaagttata tcaaaatagg aagaccaagt cttcaccatc
     2101 gacccgaatt gccaagcatc accatcgacc cataacttcg tatagcatac attatacgaa
     2161 gttatctgtg agtgaattct taggcgccgg tggagtggcg gccctcggcg cgctcgtact
     2221 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg tcggtcttgt
     2281 aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt ggtcttgacc tcggcgtcgt
     2341 agtggccgcc gtccttcagc ttcagcctca tcttgatctc gcccttcagg gcgccgtcct
     2401 cggggtacat ccgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc
     2461 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
     2521 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
     2581 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
     2641 cgtaggcctt ggagccgtac tggaactgag gggacaggat gtcccaggcg aagggcaggg
     2701 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
     2761 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgcgc accttgaagc
     2821 gcatgaactc cttgatgacg tcctcggagg aggccatgac accatcttgt tcaatctttt
     2881 ggtcctcatc attcttttca acctgtttgg ttcttctcat acagcacagc atgggccctg
     2941 ggttggactc cacgtctccc gccaacttga gaaggtcaaa attcaaagtc tgtttcactg
     3001 ccacatcgtc ctccaggctg accatctgtc ttgaggggta ggtatagcca tcctggcttc
     3061 ggcaaatgtg gagctcgtcc tctgtggtct tctggtagac tgggttgtca aagtttatgc
     3121 tgttgatgtt cttcagccgc cagttcctgt gcttgtacag ctcgtccatg ccgagagtga
     3181 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     3241 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     3301 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     3361 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     3421 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     3481 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     3541 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     3601 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     3661 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     3721 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     3781 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     3841 tgaacagctc ctcgcccttg ctcacgcttc ctcccccgcc cgtcagtttt gttgcttctt
     3901 tatccttaca ttgcacacag cccatggtgg cgtcgacgat atcctgcaga taacttcgta
     3961 taggatactt tatacgaagt tatcattggg attcttccta ttttgatcca agcatcacca
     4021 tcgaccctct agtccagatc tcaccatcga cccataactt cgtataatgt atgctatacg
     4081 aagttatgtc cctcgaagag gttcacgcgt ctgtgccttc tagttgccag ccatctgttg
     4141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4321 cggtgggctc tatgggcggc cgcgtcgaga tatctagacc cagctttctt gtacaaagtg
     4381 gtcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4441 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4501 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4561 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4621 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4681 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4741 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4801 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4921 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4981 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5041 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5101 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5161 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5221 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5281 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5341 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5401 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5461 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5521 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5581 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5641 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5701 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5761 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5821 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5881 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5941 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6001 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6061 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6121 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6181 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6241 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6301 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6361 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6421 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6781 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6841 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6901 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6961 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7021 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7081 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgct
//
