LOCUS       sequence-381579-        8271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split TEV assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
  TITLE     Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via 
            Systematic Analysis of Signal Peptide and beta-arrestin Binding 
            Motif Variants.
  JOURNAL   Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
  PUBMED    36671883
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-12-29"; volume: "13(1):48. doi"; pages: " 
            10.3390/bios13010048"
COMMENT     Sequence Label: pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..8271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2379..2403)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2798..2818
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2825..2854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2864..3304
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3347..3367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3518..3751
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3755..3781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3785..3811
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3824..3842)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3825..3842)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3862..3879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3868..4092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4138..4566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4435..4456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4575..4595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4580..4909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4760..4895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4822..4841
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4976..5770
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5030..5049)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5640..5659
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5944..6065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5981..6000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6035..6054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6114..6130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6114..6130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6127..6149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6138..6154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6162..6168,6169..6186,6187..6192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6207..6228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6345..6362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6516..7104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6596..6615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7275..8066,8067..8135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7898..7917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8136..8240)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctgcggcc gcgccaccat
      961 gaagacgatc atcgccctga gctacatctt ctgcctggta ttcgccgact acaaggacga
     1021 tgatgacgcc agcatcgata tgaggactct gaacacctct gccatggacg ggactgggct
     1081 ggtggtggag agggacttct ctgttcgtat cctcactgcc tgtttcctgt cgctgctcat
     1141 cctgtccacg ctcctgggga acacgctggt ctgtgctgcc gttatcaggt tccgacacct
     1201 gcggtccaag gtgaccaact tctttgtcat ctccttggct gtgtcagatc tcttggtggc
     1261 cgtcctggtc atgccctgga aggcagtggc tgagattgct ggcttctggc cctttgggtc
     1321 cttctgtaac atctgggtgg cctttgacat catgtgctcc actgcatcca tcctcaacct
     1381 ctgtgtgatc agcgtggaca ggtattgggc tatctccagc cctttccggt atgagagaaa
     1441 gatgaccccc aaggcagcct tcatcctgat cagtgtggca tggaccttgt ctgtactcat
     1501 ctccttcatc ccagtgcagc tcagctggca caaggcaaaa cccacaagcc cctctgatgg
     1561 aaatgccact tccctggctg agaccataga caactgtgac tccagcctca gcaggacata
     1621 tgccatctca tcctctgtaa taagctttta catccctgtg gccatcatga ttgtcaccta
     1681 caccaggatc tacaggattg ctcagaaaca aatacggcgc attgcggcct tggagagggc
     1741 agcagtccac gccaagaatt gccagaccac cacaggtaat ggaaagcctg tcgaatgttc
     1801 tcaaccggaa agttctttta agatgtcctt caaaagagaa actaaagtcc tgaagactct
     1861 gtcggtgatc atgggtgtgt ttgtgtgctg ttggctacct ttcttcatct tgaactgcat
     1921 tttgcccttc tgtgggtctg gggagacgca gcccttctgc attgattcca acacctttga
     1981 cgtgtttgtg tggtttgggt gggctaattc atccttgaac cccatcattt atgcctttaa
     2041 tgctgatttt cggaaggcat tttcaaccct cttaggatgc tacagacttt gccctgcgac
     2101 gaataatgcc atagagacgg tgagtatcaa taacaatggg gccgcgatgt tttccagcca
     2161 tcatgagcca cgaggctcca tctccaagga gtgcaatctg gtttacctga tcccacatgc
     2221 tgtgggctcc tctgaggacc tgaaaaagga ggaggcagct ggcatcgcca gacccttgga
     2281 gaagctgtcc ccagccctat cggtcatatt ggactatgac actgacgtct ctctggagaa
     2341 gatccaaccc atcacacaaa acggtcagca cccaaccgac ccagctttct tgtacaaagt
     2401 ggttgatgtc ggaggtggag gtagcggagg tggaggtagc ggagagagct tgtttaaggg
     2461 accacgtgat tacaacccga tatcgagcac catttgtcat ttgacgaatg aatctgatgg
     2521 gcacacaaca tcgttgtatg gtattggatt tggtcccttc atcattacaa acaagcactt
     2581 gtttcgtcgc aataatggaa cactgttggt ccaatcacta catggtgtat tcaaggtcaa
     2641 ggacaccacg actttgcaac aacacctcat tgatgggagg gacatgatga ttattcgcat
     2701 gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt agagagccac aaagggaaga
     2761 gcgcatatgt cttgtgacaa ccaacttcca agctcccgag aacctgtact tccagggttc
     2821 tagagaacag aagctgatct cagaagagga tctggcccgg gccatgaagc tactgtcttc
     2881 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     2941 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     3001 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     3061 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     3121 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     3181 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     3241 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     3301 atcgccggaa ttcccgggga tcagcttgat aattcgattc accatgccca agaagaagag
     3361 gaaggtggcg tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct
     3421 gctcgatctc ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc
     3481 ctttctcccc gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct
     3541 gggggacgag ctccacttag acggcgagga cgtcgcgatg gcgcatgccg acgcgctaga
     3601 cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca
     3661 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     3721 cgatgccctt ggaattgacg agtacggtgg gggctaccca tacgatgttc ctgactatgc
     3781 gggctatccc tatgacgtcc cggactatgc atgagggccc tattctatag tgtcacctaa
     3841 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4261 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4501 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4561 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4621 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4681 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4741 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4801 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4861 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4921 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     4981 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5041 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5101 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5161 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5221 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5281 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5341 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5401 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5461 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5521 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5581 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5641 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5701 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5761 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5821 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5881 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5941 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6061 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6241 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6301 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7861 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
